, Volume 47, Issue 4, pp 536–547 | Cite as

Wheat cultivars differing in heat tolerance show a differential response to monocarpic senescence under high-temperature stress and the involvement of serine proteases

  • S. Chauhan
  • S. Srivalli
  • A. R. Nautiyal
  • R. Khanna-ChopraEmail author


High temperature is a common constraint during anthesis and grain-filling stages of wheat leading to huge losses in yield. In order to understand the mechanism of heat tolerance during monocarpic senescence, the present study was carried out under field conditions by allowing two well characterized Triticum aestivum L. cultivars differing in heat tolerance, Hindi62 (heat-tolerant) and PBW343 (heat-susceptible), to suffer maximum heat stress under late sown conditions. Senescence was characterized by measuring photosynthesis related processes and endoproteolytic activity during non-stress environment (NSE) as well as heat-stress environment (HSE). There was a faster rate of senescence under HSE in both the genotypes. Hindi62, having pale yellow flag leaf with larger area, maintained cooler canopy under high temperatures than PBW343. The tolerance for high temperature in Hindi62 was clearly evident in terms of slower green-leaf area degradation, higher stomatal conductance, higher stability in maximum PSII efficiency, Rubisco activity and Rubisco content than PBW343. Both the genotypes exhibited lower endopeptidase activity under HSE as compared to NSE and this difference was more apparent in Hindi62. Serine proteases are the predominant proteases responsible for protein degradation under NSE as well as HSE. Flag leaf of both the genotypes exhibited high-molecular-mass endoproteases (78 kDa and 67 kDa) isoforms up to full grain maturity which were inhibited by specific serine protease inhibitor in both the environments. In conclusion, the heat-tolerant Hindi62 exhibited a slower rate of senescence than the heat-susceptible PBW343 during HSE, which may contribute towards heat stability.

Additional key words

heat stress photosynthesis Rubisco senescence serine protease wheat 



days after anthesis




stomatal conductance


initial fluorescence


maximum efficiency of PSII


maximum chlorophyll fluorescence of the dark adapted state


maximum chlorophyll fluorescence of the light adapted state


fresh mass


heat-stress environment


large subunit


phenylmethylsulfonyl fluoride


non-stress environment




photosynthetic photon flux density


net photosynthetic rate


photosystem II


polyvinyl pyrrolidone


polyvinyl polypyrrolidone


non-photochemical quenching




ribulose-1,5-bisphosphate carboxylase/oxygenase


sodium dodecyl sulfate — polyacrylamide gel electrophoresis


maximum temperature


mean temperature


minimum temperature


trichloroacetic acid


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This research was supported by the grants of the National Fellow scheme of Indian Council of Agricultural Research, New Delhi, India.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • S. Chauhan
    • 1
  • S. Srivalli
    • 1
  • A. R. Nautiyal
    • 2
  • R. Khanna-Chopra
    • 1
    Email author
  1. 1.Stress Physiology Laboratory, Water Technology CentreIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.High Altitude Plant Physiology Research CentreH.N.B. Garhwal UniversitySrinagar, UttarakhandIndia

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