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Photosynthetica

, Volume 47, Issue 2, pp 241–246 | Cite as

Effect of nitrogen-deficiency on midday photoinhibition in flag leaves of different rice (Oryza sativa L.) cultivars

  • E. KumagaiEmail author
  • T. Araki
  • O. Ueno
Original Paper

Abstract

Effects of nitrogen (N)-deficiency on midday photoinhibition in flag leaves were compared between two contrastive Japanese rice cultivars, a traditional japonica cultivar with low yield, cv. Shirobeniya (SRB), and a japonica-indica intermediate type with high yield, cv. Akenohoshi (AKN). Both cultivars were grown under high-N and low-N conditions. At midday, low-N supply resulted in more intensive reductions in net photosynthetic rate, stomatal conductance, maximal quantum yield of photosystem II (PSII) and quantum yield of PSII electron transport in SRB than in AKN, indicating that SRB was more strongly photoinhibited than AKN under low-N condition. At midday, the low-N plants of two cultivars showed higher superoxide dismutase (SOD) activities than the high-N plants. However, ascorbate peroxidase (APX) activity was maintained in AKN but significantly decreased in SRB under low-N condition (N-deficiency). In contrast, hydrogen peroxide (H2O2) content in SRB significantly increased under low-N condition, indicating that the susceptibility to midday photoinhibition in the low-N plants of SRB is related to the increased H2O2 accumulation. It is suggested that the midday depression in photosynthesis may be a result of oxidative stress occurring in the low-N plants in which antioxidant capacity is not enough to cope with the generation of H2O2. Therefore, H2O2-scavenging capacity could be an important factor in determining the cultivar difference of midday photoinhibition in flag leaves of rice under low-N condition.

Additional key words

antioxidative system chlorophyll fluorescence cultivar difference flag leaves hydrogen peroxide photoinhibition photoprotection rice 

Abbreviations

AKN

cv. Akenohoshi

APX

ascorbate peroxidase

Ca

atmospheric CO2 concentration

Ci

intercellular CO2 concentration

Chl

chlorophyll

Fo

initial Chl fluorescence of a dark-adapted leaf

Fm

the maximal Chl fluorescence of a dark-adapted leaf

Fm

the maximal Chl fluorescence detected in actinic light

Fs

steady Chl fluorescence in actinic light

Fv

variable Chl fluorescence

GR

glutathione reductase

gs

stomatal conductance

N

nitrogen

NBT

nitroblue tetrazolium

PN

net photosynthetic rate

PPFD

photosynthetic photon flux density

PSII

photosystem II

Rubisco

ribulose-1, 5-bisphosphate carboxylase/oxygenase

ROS

reactive oxygen species

SOD

superoxide dismutase

SRB

cv. Shirobeniya

ΦPSII

quantum yield of PSII electron transport

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References

  1. Asada, K.: The water-water cycle in chloroplasts: scavenging of active oxygen species and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 601–639, 1999.PubMedCrossRefGoogle Scholar
  2. Asada, K., Badger, M.R.: Photoreduction of 18O2 and H2 18O with concomitant evolution of 16O2 in intact spinach chloroplasts-evidence for scavenging of hydrogen-peroxide by peroxidase.-Plant Cell Physiol. 25: 1169–1179, 1984.Google Scholar
  3. Bungard, R.A., Press, M.C., Scholes, J.D.: The influence of nitrogen on rain forest dipterocarp seedings exposed to a large increase in irradiance.-Plant Cell Environ. 23: 1183–1194, 2000.CrossRefGoogle Scholar
  4. Ciompi, S., Gentili, E., Guidi, L., Soldatine, G.F.: The effect of nitrogen deficiency on leaf gas exchange and chlorophyll fluorescence parameters in sunflower.-Plant Sci. 118: 177–184, 1996.CrossRefGoogle Scholar
  5. Genty, B. Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. Biophys. Acta 990: 87–92, 1989.Google Scholar
  6. Foyer, C.H., Noctor, G.: Plant Biology-Leaves in the dark see the light.-Science 284: 599–601, 1999.PubMedCrossRefGoogle Scholar
  7. Hirasawa, T., Iida, Y., Ishihara, K.: Dominant factors in reduction of photosynthetic rate affected by air humidity and leaf water potential in rice plants.-Jap. J. Crop Sci. 58: 383–389, 1989.Google Scholar
  8. Horton, P., Murchie, E.H.: C4 photosynthesis in rice: some lessons from field studies of rice photosynthesis.-In: Sheehy, J., Mitchell, P.L., Hardy, B. (ed.): Redesigning Rice Photosynthesis to Increase Yield. Pp. 127–144, Elsevier, Amsterdam-2000.CrossRefGoogle Scholar
  9. Huang, Z.-A., Jiang, D.-A., Yang, Y., Sun, J.-W., Jin, S.-H.: Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants.-Photosynthetica 42: 357–364, 2004.CrossRefGoogle Scholar
  10. Ishida, H., Shimizu, S., Makino, A., Mae, T.: Light-dependent fragmentation of the large subunit of ribulose-1,5-bisphoshate carboxylase/oxygenase in chloroplast isolated from wheat leaves.-Planta 204: 305–309, 1998.PubMedCrossRefGoogle Scholar
  11. Jiao, D., Ji, B.: Photoinhibition in indica and japonica sub-species of rice (Oryza sativa) and their reciprocal F1 hybrids.-Aust. J. Plant Physiol. 28: 299–306, 2001.Google Scholar
  12. Jiao, D., Ji, B., Li, X.: Characteristics of chlorophyll fluorescence and membrane-lipid peroxidation during senescence of flag leaf in different cultivars of rice.-Photosynthetica 41: 33–41, 2003.CrossRefGoogle Scholar
  13. Kumagai, E., Araki, T., Kubota, F.: Effects of nitrogen supply restriction on gas exchange and photosystem 2 function in flag leaves of a traditional low-yield cultivar and a recently improved high-yield cultivar of rice (Oryza sativa L.).-Photosynthetica 45: 489–495, 2007.CrossRefGoogle Scholar
  14. Kyle, D.J., Ohad, I., Guy, R., Arntzen, C.J.: Selective thylakoid protein damage and repair during photoinhibition. In: Sybesma, C. (ed.): Advanced Photosynthesis Research. Vol. III. Pp. 67–70. Martinus Nijhoff/Dr W. Junk Publ., The Hague-Boston-Lancaster 1984.Google Scholar
  15. Long, S.P., Humphires, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 633–662, 1994.CrossRefGoogle Scholar
  16. Makino, A., Mae, T., Ohira, K.: Photosynthesis and ribulose 1,5-bisphosphate carboxylase in rice leaves: changes in photosynthesis and enzymes involved in carbon assimilation from leaf development through senescence.-Plant Physiol. 73: 1002–1007, 1983.PubMedCrossRefGoogle Scholar
  17. Mittova, V., Tal, M., Volokita, M., Guy, M.: Up-regulation of the leaf mitochondrial and peroxiomal antioxidative systems in response to salt-induced oxidative stress in the wild salttolerant tomato species Lycopersicon pennellii.-Plant Cell Environ. 26: 845–856, 2003.PubMedCrossRefGoogle Scholar
  18. Patterson, B.D., Macrae, E.A., Ferguson, I.B.: Estimation of hydrogen peroxide in plants extracts using Titanium (IV).-Anal. Biochem. 139: 487–492, 1984.PubMedCrossRefGoogle Scholar
  19. Quick, W.P. Chaves, M.M., Wendler, M.: The effect of water-stress on photosynthetic carbon metabolism in 4 species grown under field conditions.-Plant Cell Environ 15: 25–35, 1992.CrossRefGoogle Scholar
  20. Ramalho, J.C., Camos, P.S., Teixeira, M.T., Nunes, M.A.: Nitrogen-dependent changes in antioxidant system and in fatty acid composition of chloroplast membranes from Coffea arabica L. plants submitted to high irradiance.-Plant Sci. 135: 115–124, 1998.CrossRefGoogle Scholar
  21. Skillman, J.B., Osmond, C.B.: Influence of nitrogen supply and growth irradiance on photoinhibition and recovery in Heuchera americana (Saxifragaceae).-Physiol. Plant. 103: 567–573, 1998.CrossRefGoogle Scholar
  22. Takahashi, S., Murata, N.: How do environmental stresses accelerate photoinhibition?-Trends Plant Sci. 13: 178–182, 2008.PubMedCrossRefGoogle Scholar
  23. Yamane, K., Rahman, M.S., Kawasaki, M., Taniguchi M., Miyake, H.: Pretreatment with antioxidants decreases the effects of salt stress on chloroplast ultrastructure in rice leaf segments (Oryza sativa L.).-Plant Prod. Sci. 7: 292–300, 2004.CrossRefGoogle Scholar
  24. van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology.-Photosynth. Res. 25: 147–150, 1990.CrossRefGoogle Scholar
  25. Wang, Q.A., Lu, C.M., Zhang, Q.D.: Midday photoinhibition of two newly developed super-rice hybrids.-Photosynthetica 43: 277–231, 2005.CrossRefGoogle Scholar
  26. Zhou, Y.H., Yu, J.Q., Mao, W.H., Huang, L.F., Song, X.S., Nogues, S.: Genotypic variation of rubisco expression, photosynthetic electron flow and antioxidant metabolism in the chloroplasts of chill-exposed cucumber plants.-Plant Cell Physiol. 47: 192–199, 2006.PubMedCrossRefGoogle Scholar
  27. Zhou, Y.H., Lam, H.M., Zhang, J.H.; Inhibition of photosynthesis and energy dissipation induced by water and high light stresses in rice.-J. Exp. Bot. 58: 1207–1217, 2007.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Laboratory of Plant Production Physiology, Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityHigashi-ku, FukuokaJapan
  2. 2.Faculty of AgricultureKyushu UniversityHigashi-ku, FukuokaJapan

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