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Photosynthetica

, Volume 47, Issue 1, pp 79–86 | Cite as

Comparative effects of salt-stress and alkali-stress on the growth, photosynthesis, solute accumulation, and ion balance of barley plants

  • C.-W. Yang
  • H.-H. Xu
  • L.-L. Wang
  • J. Liu
  • D.-C. ShiEmail author
  • D.-L. Wang
Original Papers

Abstract

We compared the effects of salt-stresses (SS, 1: 1 molar ratio of NaCl to Na2SO4) and alkali-stresses (AS, 1: 1 molar ratio of NaHCO3 to Na2CO3) on the growth, photosynthesis, solute accumulation, and ion balance of barley seedlings, to elucidate the mechanism of AS (high-pH) damage to plants and the physiological adaptive mechanism of plants to AS. The effects of SS on the water content, root system activity, membrane permeability, and the content of photosynthetic pigments were much less than those of AS. However, AS damaged root function, photosynthetic pigments, and the membrane system, led to the severe reductions in water content, root system activity, content of photosynthetic pigments, and net photosynthetic rate, and a sharp increase in electrolyte leakage rate. Moreover, with salinity higher than 60 mM, Na+ content increased slowly under SS and sharply under AS. This indicates that high-pH caused by AS might interfere with control of Na+ uptake in roots and increase intracellular Na+ to a toxic level, which may be the main cause of some damage emerging under higher AS. Under SS, barley accumulated organic acids, Cl, SO4 2−, and NO3 to balance the massive influx of cations, the contribution of inorganic ions to ion balance was greater than that of organic acids. However, AS might inhibit absorptions of NO3 and Cl, enhance organic acid synthesis, and SO4 2− absorption to maintain intracellular ion balance and stable pH.

Additional key words

Hordeum intercellular CO2 concentration photosynthesis relative growth rate roots shoot solute accumulation stomatal conductance transpiration rate water content 

Abbreviations

Car

carotenoids

Chl

chlorophyll

Ci

internal CO2 concentration

DM

dry mass

E

transpiration rate

ELR

electrolyte leakage rate

FM

fresh mass

gs

stomatal conductance

OA

organic acid

PN

net photosynthetic rate

RGR

relative growth rate

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References

  1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.PubMedCrossRefGoogle Scholar
  2. Bethke, P.C., Drew, M.C.: Stomatal and non-stomatal components to inhibition of photosynthesis in leaves of Capsicum annuum during progressive exposure to NaCl salinity.-Plant Physiol. 99: 219–226, 1992.PubMedCrossRefGoogle Scholar
  3. Brand, J.D., Tang, C., Rathjen, A.J.: Screening rough-seeded lupins (Lupinus pilosus Murr. and Lupinus atlanticus Glads.) for tolerance to calcareous soils.-Plant Soil 245: 261–275, 2002.CrossRefGoogle Scholar
  4. Campbell, S.A., Nishio, J.N.: Iron deficiency studies of sugar beet using an improved sodium bicarbonate-buffered hydroponics growth system.-J. Plant Nutr. 23: 741–757, 2000.CrossRefGoogle Scholar
  5. Comas, L.H., Eissenstat, D.M., Lakso, A.N.: Assessing root death and root system dynamics in a study of grape canopy pruning.-New Phytol. 147: 171–178, 2000.CrossRefGoogle Scholar
  6. El-Samad, H.M.A., Shaddad, M.A.K.: Comparative effect of sodium carbonate, sodium sulphate, and sodium chloride on the growth and related metabolic activities of pea plants.-J. Plant Nutr. 19: 717–728, 1996.CrossRefGoogle Scholar
  7. Ge, Y., Li, J.D.: A preliminary study on the effects of halophytes on salt accumulation and desalination in the soil of Songnen Plain, northeast China.-Acta pratacult. sin. 1: 70–76, 1990.Google Scholar
  8. Hartung, W., Leport, L., Ratcliffe, R.G., Sauter, A., Duda, R., Turner, N.C.: Abscisic acid concentration, root pH and anatomy do not explain growth differences of chickpea (Cicer arietinum L.) and lupin (Lupinus angustifolius L.) on acid and alkaline soils.-Plant Soil 240: 191–199, 2002.CrossRefGoogle Scholar
  9. James, R.A., Munns, R., Caemmerer, S., Trejo, C., Miller, C., Condou, T.: Photosynthetic capacity is related to the cellular and subcellular partitioning of Na+, K+ and Cl in salt-affected barley and durum wheat.-Plant Cell Environ. 29: 2185–2197, 2006.PubMedCrossRefGoogle Scholar
  10. Jing, J.H., Ding, Z.R.: [Determining organic acid content.]-In: Boqinnoke, X.H. (ed.): Analysis Method of Plant Biochemistry. Pp. 264–267. Science Press, Beijing 1981. [In Chin.]Google Scholar
  11. Kawanabe, S., Zhu, T.C.: Degeneration and conservation of Aneurolepidium chinense grassland in Northern China.-J. jap. Grassland Soc. 37: 91–99, 1991.Google Scholar
  12. Kingsbury, R.W., Epstein, E., Peary, R.W.: Physiological responses to salinity in selected lines of wheat.-Plant Physiol. 74: 417–423, 1984.PubMedCrossRefGoogle Scholar
  13. Koyro, H.-W.: Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte Plantago coronopus (L.).-Environ. exp. Bot. 56: 136–146, 2006.CrossRefGoogle Scholar
  14. Läuchli, A., Lüttge, U.: Salinity in the soil environment.-In: Tanji, K.K. (ed.): Salinity: Environment-Plants-Molecules. Pp. 21–23. Kluwer Academic Publ., Boston 2002.Google Scholar
  15. Lutts, S., Kiner, J.M., Bouharmont, J.: NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance.-Ann. Bot. 78: 389–398, 1996.CrossRefGoogle Scholar
  16. Ma, H.-C., Fung, L., Wang, S.-S, Altman, A., Hütterman, A.: Photosynthetic response of Populus euphratica to salt stress.-Forest Ecol. Manage. 93: 55–61, 1997.CrossRefGoogle Scholar
  17. Munns, R.: Comparative physiology of salt and water stress.-Plant Cell Environ. 25: 239–250, 2002.PubMedCrossRefGoogle Scholar
  18. Munns, R., Tester, M.: Mechanisms of Salinity Tolerance.-Annu. Rev. Plant Biol. 59: 651–681, 2008.PubMedCrossRefGoogle Scholar
  19. Nuttall, G., Armstrong, R.D., Connor, D.J. Evaluating physicochemical constraints of calcarosols on wheat yield in the Victorian southern Mallee.-Aust. J. agr. Res. 54: 487–497, 2003.CrossRefGoogle Scholar
  20. Parida, A.K., Das, A.B.: Salt tolerance and salinity effects on plants: a review.-Ecotoxicol. environ. Safety 60: 324–349, 2005.PubMedCrossRefGoogle Scholar
  21. Shi, D., Sheng, Y.: Effect of various salt-alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors.-Environ. exp. Bot. 54: 8–21, 2005.CrossRefGoogle Scholar
  22. Shi, D., Wang, D.: Effects of various salt-alkali mixed stresses on Aneurolepidium chinense (Trin.) Kitag.-Plant Soil 271: 15–26, 2005.CrossRefGoogle Scholar
  23. Shi, D.C., Yin, L.J.: Difference between salt (NaCl) and alkaline (Na2CO3) stresses on Puccinellia tenuiflora (Griseb.) Scribn. et Merr. plants.-Acta bot. sin. 35: 144–149, 1993.Google Scholar
  24. Sultana, N., Ikeda, T., Itoh, R.: Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains.-Environ. exp. Bot. 42: 211–220, 1999.CrossRefGoogle Scholar
  25. Wang, X.L.: [Carboxylic acid.]-In: Wang, X.L. (ed.): Organic Chemistry. Pp. 149–150. Higher Education Press, Beijing 2001. [In Chin.]Google Scholar
  26. Yang, C.W., Chong, J.N., Kim, C.M., Li, C.Y., Shi, D.C., Wang, D.L.: Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions.-Plant Soil 294: 263–276, 2007.CrossRefGoogle Scholar
  27. Yang, C.W., Jianaer, A., Li, C.Y., Shi, D.C., Wang, D.L.: Comparison of the effects of salt-stress and alkali-stress on photosynthesis and energy storage of an alkali-resistant halophyte Chloris virgata.-Photosynthetica 46: 273–278, 2008a.CrossRefGoogle Scholar
  28. Yang, C.W., Wang, P., Li, C.Y., Shi, D.C., Wang, D.L.: Comparison of effects of salt and alkali stresses on the growth and photosynthesis of wheat.-Photosynthetica 46: 107–114, 2008b.CrossRefGoogle Scholar
  29. Zhu, G.L., Deng, X.W., Zuo, W.N.: Determination of free proline in plants.-Plant Physiol. Commun. 1: 35–37, 1983.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • C.-W. Yang
    • 1
  • H.-H. Xu
    • 1
  • L.-L. Wang
    • 1
  • J. Liu
    • 1
  • D.-C. Shi
    • 1
    Email author
  • D.-L. Wang
    • 1
  1. 1.Key Laboratory of Vegetation Ecology of Ministry of EducationNortheast Normal University ChangchunJilin ProvincePR China

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