Photosynthetica

, 46:193 | Cite as

Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated Zingiber officinale plantlets during their acclimation

  • Q. Z. Guan
  • Y. H. Guo
  • X. L. Sui
  • W. Li
  • Z. X. Zhang
Original Papers

Abstract

Ginger (Zingiber officinale Rosc.) plantlets were propagated in vitro and acclimated under different photosynthetic photon flux densities (60 and 250 µmol m−2 s−1 = LI and HI, respectively). Increases in chlorophyll (Chl) content and Chl a/b ratio were found under both irradiances. In vitro plantlets (day 0) exhibited a low photosynthesis, but chloroplasts from in vitro leaves contained well developed grana and osmiophillic globules. Photoinhibition in leaves formed in vitro was characterized by decrease of photochemical efficiency and quantum efficiency of photosystem 2 photochemistry in HI treatment during acclimation. The new leaves formed during acclimation in both treatments showed a higher photosynthetic capacity than the leaves formed in vitro. Also activities of antioxidant enzymes of micropropagated ginger plantlets changed during acclimation.

Additional key words

antioxidative enzymes chlorophyll content and fluorescence chloroplast ultrastructure ginger dry mass net photosynthetic rate photochemical quenching plant height 

References

  1. Aebi, H.: Catalase in vitro.-Meth. Enzymol. 105: 121–126, 1984.PubMedCrossRefGoogle Scholar
  2. Ali, M.B., Hahn, E., Paek, K.: Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet.-Environ. exp. Bot. 54: 109–120, 2005.CrossRefGoogle Scholar
  3. Amâncio, S., Rebordõ, J.P., Chaves, M.M.: Improvement of acclimatization of micropropagated grapevine: Photosynthetic competence and carbon allocation.-Plant Cell Tissue Organ Cult. 58: 31–37, 1999.CrossRefGoogle Scholar
  4. Bai, X.F., Jiang, X.M., Zhu, J.J., Liang, J.G.: Studies on the photosynthetic characteristics of taro test-tube plantlets after transplantation.-Acta hort. sin. 32: 518–520, 2005.Google Scholar
  5. Bryer, W.F., Fridovich, I.: Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.-Anal. Biochem. 161: 559–566, 1987.CrossRefGoogle Scholar
  6. Carvalho, L.C., Osório, M.L., Chaves, M.M., Amâncio, S.: Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization.-Plant Cell Tissue Organ Cult. 67: 271–280, 2001.CrossRefGoogle Scholar
  7. Chance, B.: Assay of catalases and peroxidases.-Meth. Enzymol. 2: 764–775, 1955.CrossRefGoogle Scholar
  8. Dekker, A.J., Rao, A.N., Gob, C.J.: In vitro storage of multiple shoot cultures of ginger at ambient temperatures of 24–29 °C.-Sci. Hort. 47: 157–167, 1991.CrossRefGoogle Scholar
  9. Estrada-Luna, A.A., Davies, F.T., Jr., Egilla, J.N.: Physiological changes and growth of micropropagated chile ancho pepper plantlets during acclimatization and post-acclimatization.-Plant Cell Tissue Organ Cult. 66: 17–24, 2001.CrossRefGoogle Scholar
  10. Genoud, C., Sallanon, H., Hitmi, A., Maziere, Y., Coudret, A.: Growth, stomatal conductance, photosynthetic rate, ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activites during rooting and acclimatisation of Rosa hybrida plantlets.-Photosynthetica 38: 629–634, 2000.CrossRefGoogle Scholar
  11. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87–92, 1989.Google Scholar
  12. Grout, B.W.W.: Photosynthesis of regenerated plantlets in vitro and the stresses of transplanting.-Acta Hort. 230: 129–135, 1988.Google Scholar
  13. Guo, Y.H., Bai, J.H., Zhang, Z.X.: Plant regeneration from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc.).-Plant Cell Tissue Organ Cult. 89: 151–157, 2007.CrossRefGoogle Scholar
  14. Guo, Y.H., Zhang, Z.X.: Establishment and plant regeneration of somatic embryogenic cell suspension cultures of the Zingiber officinale Rosc.-Sci. Hort. 107: 90–96, 2005.CrossRefGoogle Scholar
  15. Hewitt, E.J., Smith, T.A.: Plant Mineral Nutrition.-The English Universities Press, London 1975.Google Scholar
  16. Hosoki, T., Sagawa, Y.: Clonal propagation of ginger (Zingiber officinale Rosc.) through tissue culture.-HortScience 12: 451–452, 1977.Google Scholar
  17. Hossain, M.A., Asada, K.: Purification of dehydroascorbate reductase from apinach and its characterization as a thiol enzyme.-Plant Cell Physiol. 25: 85–92, 1984.Google Scholar
  18. Kackar, A., Bhat, S.R., Chandel, K.P.S., Malik, S.K.: Plant regeneration via somatic embryogenesis in ginger.-Plant Cell Tissue Organ Cult. 32: 289–292, 1993.CrossRefGoogle Scholar
  19. Lamhamedi, M.S., Chamberland, H., Tremblay, F.M.: Epidermal transpiration, ultrastructural characteristics and net photosynthesis of white spruce somatic seedlings in response to in vitro acclimatization. Physiol. Plant. 118: 554–561, 2003.CrossRefGoogle Scholar
  20. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350–382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987.Google Scholar
  21. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.CrossRefGoogle Scholar
  22. Nguyen, Q.T., Kozai, T., Niu, G., Nguyen, U.V.: Photosynthetic characteristics of coffee (Coffea arabica) plantlets in vitro in response to different CO2 concentrations and light intensities.-Plant Cell Tissue Organ Cult. 55: 133–139, 1999.CrossRefGoogle Scholar
  23. Osório, M.L., Gonçalves, S., Osório, J., Romano, A.: Effects of CO2 concentration on acclimatization and physiological responses of two cultivars of carob tree.-Biol. Plant. 49: 161–167, 2005.CrossRefGoogle Scholar
  24. Piqueras, A., Debergh, P.C.: The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants.-Plant Sci. 155: 59–66, 2000.PubMedCrossRefGoogle Scholar
  25. Piqueras, A., Van Huylenbroeck, J.M., Han, B.H., Debergh, P.C.: Carbohydrate partitioning and metabolism during acclimatization of micropropagated Calathea.-Plant Growth Regul. 26: 25–31, 1998.CrossRefGoogle Scholar
  26. Pospíšilová, J., Tichá, I., Kadleček, P., Haisel, D., Plzáková, Š.: Acclimation of micropropated plants to ex vitro conditions.-Biol. Plant. 42: 481–497, 1999.CrossRefGoogle Scholar
  27. Seon, J.H., Cui, Y.Y., Kozai, T., Paek, K.Y.: Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period.-Plant Cell Tissue Organ Cult. 61: 135–142, 2000.CrossRefGoogle Scholar
  28. Serret, M.D., Trillas, M.I., Matas, I., Araus, J.L.: Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation.-Plant Cell Tissue Organ Cult. 45: 1–16, 1996.CrossRefGoogle Scholar
  29. Sharma, T.R., Singh, B.M.: High-frequency in vitro multiplication of disease-free Zingiber oficinale Roscae.-Plant Cell Rep. 17: 68–72, 1997.CrossRefGoogle Scholar
  30. Triques, K., Rival, A., Beulé, T., Puard, M., Roy, J., Nato, A., Lavergne, D., Havaux, M., Verdeil, J.-L., Sangare, A., Hamon, S.: Photosynthetic ability of in vitro grown coconut (Cocos nucifera L.) plantlets derived from zygotic embryos.-Plant Sci. 127: 39–51, 1997.CrossRefGoogle Scholar
  31. Van Huylenbroeck, J.M., Debergh, P.C.: Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets.-Biol. Plant. 96: 298–304, 1996.Google Scholar
  32. Van Huylenbroeck, J.M., Piqueras, A., Debergh, P.C.: Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants.-Plant Sci. 134: 21–30, 1998.CrossRefGoogle Scholar
  33. Van Huylenbroeck, J.M., Piqueras, A., Debergh, P.C.: The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants.-Plant Sci. 155: 59–66, 2000.PubMedCrossRefGoogle Scholar
  34. Van Huylenbroeck, J.M., van Laere, I.M.B., Piqueras, A., Debergh, P.C., Bueno, P.: Time course of catalase and superoxide dismutase during acclimatization and growth of micropropagated Calathea and Spathiphyllum plants.-Plant Growth Reg. 26: 7–14, 1998.CrossRefGoogle Scholar
  35. Wetzstein, H.Y., Sommer, H.E.: Leaf anatomy of tissue cultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization.-Amer. J. Bot. 69: 1579–1586, 1982.CrossRefGoogle Scholar
  36. Yue, D., Gosselin, A., Desjardins, Y.: Re-examination of the photosynthetic capacity of in vitro-cultured strawberry plantlets.-J. amer. Soc. hort. Sci. 118: 419–424, 1993.Google Scholar
  37. Zhao, D.W.: High Quality and Production of Ginger-Theory and Technology. Pp. 10–30. China Agricultural Publishing Company, Beijing 2002.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR, Praha 2008

Authors and Affiliations

  • Q. Z. Guan
    • 1
  • Y. H. Guo
    • 1
  • X. L. Sui
    • 1
  • W. Li
    • 1
  • Z. X. Zhang
    • 1
  1. 1.College of Agronomy and BiotechnologyChina Agriculture UniversityBeijingChina

Personalised recommendations