Advertisement

Photosynthetica

, Volume 46, Issue 1, pp 35–39 | Cite as

Photosystem 2 photochemistry and pigment composition of a yellow mutant of rice (Oryza sativa L.) under different irradiances

  • Q. Chen
  • L. F. Wang
  • N. Su
  • H. D. Qin
  • H. B. Niu
  • J. L. Wang
  • H. Q. Zhai
  • J. M. Wan
Original Papers

Abstract

A yellow leaf colouration mutant (named ycm) generated from rice T-DNA insertion lines was identified with less grana lamellae and low thylakoid membrane protein contents. At weak irradiance [50 µmol(photon) m−2 s−1], chlorophyll (Chl) contents of ycm were ≈20 % of those of WT and Chl a/b ratios were 3-fold that of wild type (WT). The leaf of ycm showed lower values in the actual photosystem 2 (PS2) efficiency (ΦPS2), photochemical quenching (qP), and the efficiency of excitation capture by open PS2 centres 1 (Fv′/Fm′) than those of WT, except no difference in the maximal efficiency of PS2 photochemistry (Fv/Fm). With progress in irradiance [100 and 200 µmol(photon) m−2 s−1], there was a change in the photosynthetic pigment stoichiometry. In ycm, the increase of total Chl contents and the decrease in Chl a/b ratio were observed. ΦPS2, qP, and Fv′/Fm′ of ycm increased gradually along with the increase of irradiance but still much less than in WT. The increase of xanthophyll ratio [(Z+A)/(V+A+Z)] associated with non-photochemical quenching (qN) was found in ycm which suggested that ycm dissipated excess energy through the turnover of xanthophylls. No significant differences in pigment composition were observed in WT under various irradiances, except Chl a/b ratio that gradually decreased. Hence the ycm mutant developed much more tardily than WT, which was caused by low photon energy utilization independent of irradiance.

Additional key words

β-carotene chlorophyll photochemical and non-photochemical quenching xanthophyll cycle 

Abbreviations

A

antheraxanthin

Chl

chlorophyll

F0

minimal fluorescence in dark-adapted leaves

Fm

maximal fluorescence in dark-adapted leaves

Fv

maximum variable fluorescence in dark-adapted leaves

F0

minimal fluorescence in light-adapted leaves

Fm

maximal fluorescence in light-adapted leaves

Fv

maximum variable fluorescence in light-adapted leaves

Fv/Fm

maximal efficiency of PS2 photochemistry

Fv′/Fm

efficiency of excitation energy capture by open PS2 reaction centres

PS

photosystem

qN

non-photochemical quenching

qP

photochemical quenching

V

violaxanthin

Z

zeaxanthin

ΦPS2

actual PS2 efficiency

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Campbell, D., Hurry, V., Clarke, A.K., Gustafsson, P., Öquist, G.: Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation.-Microbiol. mol. Biol. Rev. 62: 667–683, 1998.PubMedGoogle Scholar
  2. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599–626, 1992.CrossRefGoogle Scholar
  3. Demmig-Adams, B., Adams, W.W., III: The role of xanthophyll cycle carotenoids in the protection of photosynthesis.-Trends Plant Sci. 1: 21–26, 1996.CrossRefGoogle Scholar
  4. Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253–264, 1996.CrossRefGoogle Scholar
  5. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87–92, 1989.Google Scholar
  6. Hsu, B.D., Lee, J.Y.: The photosystem II heterogeneity of chlorophyll b-deficient mutants of rice: a fluorescence induction study.-Plant Physiol. 22: 195–200, 1995.Google Scholar
  7. Jung, K.H., Hur, J.H., Ryu, C.H., Choi, Y.J., Chung, Y.Y., Miyao, A., Hirochika, H., An, G.H.: Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system.-Plant Cell Physiol. 44: 463–472, 2003.PubMedCrossRefGoogle Scholar
  8. Krause, G.H., Cornic, G.: CO2 and O2 interactions in photoinhibition.-In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 169–196. Elsevier, Amsterdam-New York-Oxford 1987.Google Scholar
  9. Kusumi, K., Komori, H., Satoh, H., Iba, K.: Characterization of a zebra mutant of rice with increased susceptibility to light stress.-Plant Cell Physiol. 41: 158–64, 2000.PubMedGoogle Scholar
  10. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–685, 1970.PubMedCrossRefGoogle Scholar
  11. Lee, S., Kim, J.H., Yoo, E.S., Lee, C.H., Hirohiko, H., An, G.H.: Differential regulation of chlorophyll a oxygenase genes in rice.-Plant mol. Biol. 57: 805–818, 2005.PubMedCrossRefGoogle Scholar
  12. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic membranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350–382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987.Google Scholar
  13. Lu, C.M., Lu, Q.T., Zhang, J.H., Kuang, T.Y.: Characterization of photosynthetic pigment composition, Photosystem II photochemistry and thermal energy dissipation during leaf senescence of wheat plants grown in the field.-J. exp. Bot. 52: 1805–1810, 2001.PubMedCrossRefGoogle Scholar
  14. Lu, C.M., Zhang, J.H.: Heat-induced multiple effects on PSII in wheat plants.-J. Plant Physiol. 156: 259–265, 2000a.Google Scholar
  15. Lu, C.M., Zhang, J.H.: Photosystem II photochemistry and its sensitivity to heat stress in maize plants as affected by nitrogen deficiency.-J. Plant Physiol. 157: 124–130, 2000b.Google Scholar
  16. Masuda, T., Fusada, N., Oosawa, N., Takamatsu, K., Yamamoto, Y.Y., Ohto, M., Nakamura, K., Goto, K., Shibata, D., Shirano, Y., Hayashi, H., Kato, T., Tabata, S., Shimada, H., Ohta, H., Takamiya, K.: Functional analysis of isoforms of NADPH:protochlorophyllide oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana.-Plant Cell Physiol. 44: 963–974, 2003.PubMedCrossRefGoogle Scholar
  17. Öquist, G., Huner, N.P.A.: Cold-hardening-induced resistance to photoinhibition of photosynthesis in winter rye is dependent upon an increased capacity for photosynthesis.-Planta 189: 150–156, 1993.CrossRefGoogle Scholar
  18. Peng, L.W., Ma, J.F., Chi, W., Guo, J.K., Zhu, S.Y., Lu, Q.T., Lu, C.M., Zhang, L.X.: Low PS2 ACCUMULATION1 is involved in efficient assembly of photosystem II in Arabidopsis thaliana.-Plant Cell 18: 955–969, 2006.PubMedCrossRefGoogle Scholar
  19. Sugimoto, H., Kusumi, K., Tozawa, Y., Yazaki, J., Kishimoto, N., Kikuchi, S., Iba, K.: The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation.-Plant Cell Physiol. 45: 985–996, 2004.PubMedCrossRefGoogle Scholar
  20. Terao, T., Yamashita, A., Katoh, S.: Chlorophyll b-deficient mutants of rice. I. Absorption and fluorescence spectra and chlorophyll a/b ratios.-Plant Cell Physiol. 26: 1361–1367, 1985.Google Scholar
  21. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331–343, 1990.CrossRefGoogle Scholar
  22. Wang, L.F., Ji, H.B., Bai, K.Z., Li, L.B., Kuang, T.Y.: Photosystem 2 activities of hyper-accumulator Dicranopteris dichotoma Bernh from a light rare earth elements mine.-Photosynthetica 44: 202–207, 2006.CrossRefGoogle Scholar
  23. Zhang, H.T., Li, J.J., Yoo, J.H., Yoo, S.C., Cho, S.H., Koh, H.J., Seo, H.S., Paek, N.C.: Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development.-Plant mol. Biol. 62: 325–337, 2006.PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2008

Authors and Affiliations

  • Q. Chen
    • 1
  • L. F. Wang
    • 1
  • N. Su
    • 1
  • H. D. Qin
    • 2
  • H. B. Niu
    • 2
  • J. L. Wang
    • 1
  • H. Q. Zhai
    • 1
  • J. M. Wan
    • 1
    • 2
  1. 1.Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina

Personalised recommendations