Photosynthetica

, Volume 45, Issue 4, pp 606–611

Fluorescence excitation spectra of drought resistant and sensitive genotypes of triticale and maize

  • M. T. Grzesiak
  • A. Rzepka
  • T. Hura
  • S. Grzesiak
  • K. Hura
  • W. Filek
  • A. Skoczowski
Original Papers

Abstract

An influence of soil drought (7 or 14 d) and 7 d recovery on changes of leaf fluorescence excitation spectra at wavelengths of 450, 520, 690, and 740 nm (F450, F520, F690, F740) for drought resistant and sensitive genotypes of triticale and maize was compared. In non-stressed plants the differences between maize and triticale were observed for F450 and F520, but not for F690 and F740. Drought caused the increase in F450, F520, and F690 and this increase was more distinct for drought sensitive genotypes. After re-hydration, chlorophyll fluorescence mostly recovered to values of control plants. Drought caused significant increase in F690/F740 but not in F450/F690 and F450/F520. For triticale, highest increase in F690/F740 was observed in the 4th and 7th leaves of resistant genotype and contrarily in maize for the sensitive one. After recovery, the F450/F520, F450/F690, and F690/F740 ratios mostly returned to values of control plants.

Additional key words

Triticale Zea 

Abbreviations

Chl

chlorophyll

FWC

field water capacity

PAR

photosynthetically active radiation

PS

photosystem

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bilger, W., Johnsen, T., Schreiber, U.: UV-excited chlorophyll fluorescence as a tool for the assessment of UV-protection by the epidermis of plants.-J. exp. Bot. 52: 2007–2014, 2001.PubMedCrossRefGoogle Scholar
  2. Buschmann, C., Langsdorf, G., Lichtenthaler, H.K.: Imaging of the blue, green, and red fluorescence emission of plants: An overview.-Photosynthetica 38: 483–491, 2000.CrossRefGoogle Scholar
  3. Buschmann, C., Lichtenthaler, H.K.: Principles and characteristics of multi-colour fluorescence imaging of plants.-J. Plant Physiol. 152: 297–314, 1998.Google Scholar
  4. Cerovic, Z.G., Ounis, A., Cartelat, A., Latouche, G., Goulas, Y., Meyer, S., Moya, I.: The use of chlorophyll fluorescence excitation spectra for the non-destructive in situ assessment of UV-absorbing compounds in leaves.-Plant Cell Environ. 25: 1663–1676, 2002.CrossRefGoogle Scholar
  5. Chaves, M.M., Maroco, J.P., Pereira, J.S.: Understanding plant responses to drought-from genes to whole plant.-Funct. Plant Biol. 30: 239–264, 2003.CrossRefGoogle Scholar
  6. Cornic, G., Massacci, A.: Leaf photosynthesis under drought stress.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 347–366. Kluwer Academic Publ., Dordrecht-Boston-London 1996.Google Scholar
  7. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599–626, 1992.CrossRefGoogle Scholar
  8. Fischer, R.A., Maurer, R.: Drought resistance in spring wheat cultivars. I. Grain yield responses.-Aust. J. agr. Res. 29: 897–912, 1978.CrossRefGoogle Scholar
  9. Grzesiak, M.T.: [Effect of Drought Stress on Photosynthetic Apparatus and Productivity of Triticale and Maize Genotypes Differing in Drought Tolerance.]-Doctor Thesis. Cracow Agricultural University, Cracow 2004. [In Polish.]Google Scholar
  10. Grzesiak, M.T., Grzesiak, S., Skoczowski, A.: Changes of leaf water potential and gas exchange during and after drought in triticale and maize genotypes differing in drought tolerance.-Photosynthetica 44: 561–568, 2006.CrossRefGoogle Scholar
  11. Grzesiak, M.T., Rzepka, A., Hura, T., Hura, K., Skoczowski, A.: Changes in response to drought stress of triticale and maize genotypes differing in drought tolerance.-Photosynthetica 45: 280–287, 2007.CrossRefGoogle Scholar
  12. He, P., Osaki, M., Takebe, M., Shinano, T.: Changes of photosynthetic characteristics in relation to leaf senescence in two maize hybrids with different senescent appearance.-Photosynthetica 40: 547–552, 2002.CrossRefGoogle Scholar
  13. Hideg, E., Juhasz, M., Bornman, J.F., Asada, K.: The distribution and possible origin of blue-green fluorescence in control and stressed barley leaves.-Photochem. photobiol. Sci. 1: 934–941, 2002.PubMedCrossRefGoogle Scholar
  14. Lang, M.: Studies on the blue-green and chlorophyll fluorescence of plants and their applications for fluorescence imaging of leaves.-Plant Physiol. 29: 1–110, 1995.Google Scholar
  15. Lang, M., Lichtenthaler, H.K.: Changes in the blue-green and red fluorescence-emission spectra of beech leaves during the autumnal chlorophyll breakdown.-J. Plant Physiol. 138: 550–553, 1995.Google Scholar
  16. Lang, M., Lichtenthaler, H.K., Sowinska, M., Heisel, F., Miehé, J.A.: Fluorescence imaging of water and temperature stress in plant leaves.-J. Plant Physiol. 148: 613–621, 1996.Google Scholar
  17. Lang, M., Lichtenthaler, H.K., Sowinska, M., Summ, P., Heisel, F.: Blue, green and red fluorescence signatures and images of tabacco leaves.-Bot. Acta 107: 230–236, 1994.Google Scholar
  18. Lichtenthaler, H.K.: Vegetation stress: an introduction to the stress concept in plants.-J. Plant Physiol. 148: 4–14, 1996.Google Scholar
  19. Lichtenthaler, H.K.: In vivo chlorophyll fluorescence as a tool for stress detection in plants.-In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 129–142. Kluwer Academic Publ., Dordrecht 1998.Google Scholar
  20. Lichtenthaler, H.K., Buschmann, C., Knapp, M.: How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll decrease ratio RFd of leaves with the PAM fluorometer.-Photosynthetica 43: 379–393, 2005.CrossRefGoogle Scholar
  21. Lichtenthaler, H.K., Lang, M., Sowinska, M., Heisel, F., Miehé, J.A.: Detection of vegetation stress via a new high resolution fluorescence imaging system.-J. Plant Physiol. 148: 599–612, 1996.Google Scholar
  22. Lichtenthaler, H.K., Schweiger, J.: Cell wall bound ferulic acid, the major substance of the blue-green fluorescence emission of plants.-J. Plant Physiol. 152: 272–282, 1998.Google Scholar
  23. Meyer, S., Cartelat, A., Moya, I., Cerovic, Z.G.: UV-induced blue-green and far-red fluorescence of long wheat leaves: a potential signature of leaf ageing.-J. exp. Bot. 54: 757–769, 2003.PubMedCrossRefGoogle Scholar
  24. Morales, F., Cartelat, A., Álvarez-Fernández, A., Moya, I., Cerovic, Z.G.: Time-resolved spectral studies of blue-green fluorescence of artichoke (Cynara cardunculus L. var. scolymus) leaves: identification of chlorogenic acid as one of the major fluorophores and age-mediated changes.-J. agr. Food Chem. 53: 9668–9678, 2005.CrossRefGoogle Scholar
  25. Morales, F., Cerovic, Z.G., Moya, I.: Characterization of blue-green fluorescence in the mesophyll of sugar beet (Beta vulgaris L.) leaves affected by iron deficiency.-Plant Physiol. 106: 127–133, 1994.PubMedGoogle Scholar
  26. Schmitz-Hoerner, R., Weissenbock, G.: Contribution of phenolic compounds to the UV-B screening capacity of developing barley primary leaves in relation to DNA damage and repair under elevated UV-B levels.-Phytochemistry 64: 243–55, 2003.PubMedCrossRefGoogle Scholar
  27. Schweiger, J., Lang, M., Lichtenthaler, H.K.: Differences in fluorescence excitation spectra of leaves between stressed and non-stressed plants.-J. Plant Physiol. 148: 536–547, 1996.Google Scholar
  28. Šesták, Z., Šiffel, P.: Leaf-age related differences in chlorophyll fluorescence.-Photosynthetica 33: 347–369, 1997.Google Scholar
  29. Stober, F., Lichtenthaler, H.K.: Changes of the laser-induced blue, green and red fluorescence signatures during greening of etiolated leaves of wheat.-J. Plant Physiol. 140: 673–680, 1998.Google Scholar
  30. van Rensburg, L., Krüger, G.H.J.: Differential inhibition of photosynthesis (in vivo and in vitro), and changes in chlorophyll a fluorescence induction kinetics of four tobacco cultivars under drought stress.-J. Plant Physiol. 141: 357–365, 1993.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • M. T. Grzesiak
    • 1
  • A. Rzepka
    • 2
  • T. Hura
    • 1
  • S. Grzesiak
    • 1
  • K. Hura
    • 3
  • W. Filek
    • 3
  • A. Skoczowski
    • 1
  1. 1.The Franciszek Górski Institute of Plant PhysiologyPolish Academy of SciencesCracowPoland
  2. 2.Department of Plant Physiology, Institute of BiologyPedagogical AcademyCracowPoland
  3. 3.Department of Plant PhysiologyCracow Agricultural UniversityCracowPoland

Personalised recommendations