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Photosynthetica

, Volume 45, Issue 3, pp 334–339 | Cite as

Diurnal regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity and its content in Norway spruce needles

  • M. HrstkaEmail author
  • O. Urban
  • E. Petrů
  • L. Babák
Original Papers

Abstract

Diurnal changes of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activity and its content were measured to find the mechanism of RuBPCO activity regulation in Norway spruce needles. Both initial and total RuBPCO activities as well as the activation state had a typical pattern with two peaks in the morning and afternoon, respectively, and a midday depression. On the 19 October, RuBPCO content decreased during the day from 3.1 to 1.4 g m−2, while on the 20 October it was approximately constant both in the morning and in the afternoon (2.7 g m−2). Neither initial nor total activity of RuBPCO copied irradiances. Relatively low morning and evening values of total activities indicate that nocturnal inhibitor CA1P is important in Norway spruce. However, the midday depression of total activity indicates that besides CA1P there function some other inhibitors of RuBPCO. In addition, the diminution of RuBPCO content during the day may indicate repression of its gene expression.

Additional key words

CA1P daily inhibitors midday depression Picea 

Abbreviations

Ci

intercellular CO2 partial pressure

CA1P

2-carboxy-D-arabinol-1-phosphate

DTT

dithiothreitol

HEPES

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

PPFD

photosynthetic photon flux density

RuBP

D-ribulose-1,5-bisphosphate

RuBPCO

ribulose-1,5-bisphosphate carboxylase/oxygenase

SDS

sodium dodecyl sulfate

Tair

air temperature

TCA

trichloroacetic acid

Tris

Tris[hydroxymethyl] aminomethane

VPD

vapour pressure deficit

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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Faculty of ChemistryBrno University of TechnologyBrnoCzech Republic
  2. 2.Institute of Systems Biology and EcologyAcademy of Sciences of the Czech RepublicBrnoCzech Republic

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