Advertisement

Photosynthetica

, Volume 43, Issue 3, pp 439–444 | Cite as

Estimating heat tolerance among plant species by two chlorophyll fluorescence parameters

  • J.-H. Weng
  • M.-F. Lai
Article

Abstract

The heat tolerance of 8 temperate- and 1 subtropical-origin C3 species as well as 17 tropical-origin ones, including C3, C4, and CAM species, was estimated using both F0-T curve and the ratio of chlorophyll fluorescence parameters, prior to and after high temperature treatment. When leaves were heated at the rate of ca. 1 °C min−1 in darkness, the critical temperature (Tc) varied extensively among species. The Tc's of all 8 temperate-origin species ranged between 40–46 °C in winter (mean temperature 16–19 °C), and between 32–48 °C in summer (mean temperature ca. 30 °C). Those for 1 subtropical- and 12 tropical-origin C3 species ranged between 25–44 °C and 35–48 °C, and for 1 CAM and 4 C4 species were 41–47 and 45–46 °C, respectively. Acclimating three C3 herbaceous plants at high temperature (33/28 °C, day/night) for 10 d in winter caused their Tc's rising to nearly the values measured in summer. When leaves were exposed to 45 °C for 20 min and then kept at room temperature in darkness for 1 h, a significant correlation between RFv/m (the ratio of Fv/Fm before and after 45 °C treatment) and Tc was observed for all tested temperate-origin C3 species as well as tropical-origin CAM and C4 species. However, F0 and Fv/Fm of the tropical-origin C3 species were less sensitive to 45 °C treatment, regardless of a large variation of Tc; thus no significant correlation was found between their RFv/m and Tc. Thus Tc might not be a suitable index of heat tolerance for plants with wide range of environmental adaptation. Nevertheless, Tc's of tropical origin C3 species, varying and showing high plasticity to seasonal changes and temperature treatment, appeared suitable for the estimation of the degree of temperature acclimation in the same species.

Additional key words

C3, C4, and CAM plants species differences in fluorescence temperate origin thermo-tolerance tropical origin 

Abbreviations

Chl

chlorophyll

F0

basic chlorophyll fluorescence

Fm

maximum chlorophyll fluorescence

Fv/Fm

potential efficiency of PS2

PPFD

photosynthetic photon flux density

PS2

photosystem 2

Tc

temperature at the start of F0 sharp increase

Tp

temperature at maximum F0

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ball, M.C., Butterworth, J.A., Roden, J.S., Christian, R., Egerton, J.J.G.: Application of chlorophyll fluorescence to forest ecology. — Aust. J. Plant Physiol. 22: 311–319, 1994.Google Scholar
  2. Berry, J., Bjorkman, O.: Photosynthetic response and adaptation to temperature in hiher plants. — Annu. Rev. Plant Physiol. 31: 491–543, 1980.CrossRefGoogle Scholar
  3. Bilger, H.-W., Schreiber, U., Lange, O.L.: Determination of leaf heat resistance: comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods. — Oecologia 63: 256–262, 1984.CrossRefGoogle Scholar
  4. Bjorkman, O., Badger, M.R., Armond, M.D.: Response and adaptation of photosynthesis to high temperatures. — In: Turner, N.C., Kramer, P.J. (ed.): Adaptations of Plants to Water and High Temperature Stress. Pp. 233–249. Wiley-Interscience, New York — Chichester — Brisbane — Toronto 1980.Google Scholar
  5. Braun, V., Buchner, O., Neuner, G.: Thermotolerance of photosystem 2 of three alpine plant species under field conditions. — Photosynthetica 40: 587–595, 2002.CrossRefGoogle Scholar
  6. Downton, W.J.S., Berry, J.A., Seemann, J.R.: Tolerance of photosynthesis to high temperature in desert plants. — Plant Physiol. 74: 786–790, 1984.Google Scholar
  7. Kitao, M., Lei, T.T., Koike, T., Tobita, H., Maruyama, Y., Matsumoto, Y., Ang, L.-H.: Temperature response and photo-inhibition investigated by chlorophyll fluorescence measurements for four distinct species of dipterocarp trees. — Physiol. Plant. 109: 284–290, 2000.CrossRefGoogle Scholar
  8. Knight, C.A., Ackerly, D.D.: An ecological and evolutionary analysis of photosynthetic thermotolerance using the temperature-dependent increase in fluorescence. — Oecologia 130: 505–514, 2002.CrossRefGoogle Scholar
  9. Koniger, M., Harris, G.C., Pearcy, R.W.: Interaction between photon flux density and elevated temperatures on photo-inhibition in Alocasia macrorrhiza. — Planta 205: 214–222, 1998.CrossRefGoogle Scholar
  10. Kuropatwa, R., Naus, J., Maslan, M., Dvorak, L.: Basic properties of the chlorophyll fluorescence temperature curve in barley leaves. — Photosynthetica 27: 129–138, 1992.Google Scholar
  11. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence — a practical guide. — J. exp. Bot. 51: 659–668, 2000.CrossRefPubMedGoogle Scholar
  12. Schreiber, U., Berry, J.A.: Heat-induced changes of chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus. — Planta 136: 233–238, 1977.CrossRefGoogle Scholar
  13. Schwarz, A.G., Redmann, R.E.: Photosynthetic properties of C4 grass (Spartina gracilis Trin.) from northern environment. — Photosynthetica 23: 449–459, 1989.Google Scholar
  14. Seemann, J.R., Downton, W.J.S., Berry, J.A.: Temperature and leaf osmotic potential as factors in the acclimation of photosynthesis to high temperature in desert plants. — Plant Physiol. 80: 926–930, 1986.Google Scholar
  15. Slatyer, R.O., Morrow, P.A.: Altitudinal variation in the photosynthetic characteristics of snow gum, Eucalyptus pauciflora Sieb. ex Spreng. II. Effects of growth temperature under controlled conditions. — Aust. J. Plant Physiol. 4: 289–299, 1977.Google Scholar
  16. Smillie, R.M., Nott, R.: Heat injury of leaves of alpine, temperate and tropical plants. — Aust. J. Plant Physiol. 6: 135–141, 1979.Google Scholar
  17. Weis, E., Berry, J.A.: Plants and high temperature stress. — In: Long, S.P., Woodward, F.I. (ed.): Plants and Temperature. Pp. 329–346. Company of Biologists, Cambridge 1988.Google Scholar
  18. Weng, J.-H., Ueng, R.-G.: Effect of temperature on photosynthesis of Miscanthus clones collected from different elevations. — Photosynthetica 34: 307–311, 1997.CrossRefGoogle Scholar
  19. Yamada, M., Hidaka, T., Fukamachi, H.: Heat tolerance in leaves of tropical fruit crops as measured by chlorophyll fluorescence. — Sci. Hortic. 67: 39–48, 1996.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  • J.-H. Weng
    • 1
  • M.-F. Lai
    • 1
  1. 1.Department of Life ScienceNational Chung-Hsing UniversityTaichungTaiwan

Personalised recommendations