Polyelectrolyte Carboxymethyl Cellulose for Enhanced Delivery of Doxorubicin in MCF7 Breast Cancer Cells: Toxicological Evaluations in Mice Model
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Chemotherapy as an important tool for cancer treatment faces many obstacles such as multidrug resistance and adverse toxic effects on healthy tissues. Drug delivery systems have opened a new window to overcome these problems.
A polyelectrolyte carboxymethyl cellulose polymer as a magnetic nanocarrier was synthesized for enhancing delivery and uptake of doxorubicin in MCF7 breast cancer cells and decreasing the adverse toxic effects to healthy tissues.
The physicochemical properties of developed nanocarrier showed that it can be used in drug delivery purposes. The efficiency of the delivery system was assessed by loading and release studies. Besides, biological assays including protein-particle interaction, hemolysis assay, cytotoxicity study, cellular uptake, and apoptosis analysis were performed. All results persuaded us to investigate the cytotoxic effects of nanocarrier in an animal model by determining the biochemical parameters attributed to organ injuries, and hematoxylin and eosin (H&E) staining for histopathological manifestations. We observed that the nanocarrier has no toxic effect on healthy tissues, while, it is capable of reducing the toxic side effects of doxorubicin by more cellular internalization.
Chemical characterizations and biological studies confirmed that developed nanocarrier with permanent cationic groups of imidazolium and anionic carboxylic acid groups is an effective candidate for anticancer drug delivery.
KEY WORDSenhanced drug delivery doxorubicin toxicity MCF7 breast cancer cells polyelectrolyte carboxymethyl cellulose
Drug delivery systems
Drug encapsulation efficiency
Drug loading efficiency
Dynamic light scattering
Differential scanning calorimetry
Energy-dispersive X-ray spectroscopy
Enhanced permeability and retention
Fetal bovine serum
Fourier transform infrared PLGA
Mean fluorescent intensity
3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphe-nyltetrazolium bromide
Roswell Park Memorial Institute 1640 growth medium
Scanning electron microscopy
Transmission Electron Microscopy
Acknowledgments and Disclosures
We thank the Drug Applied Research Centre (DARC), Aging Research Institute, Physical Medicine and Rehabilitation Research Centre, Clinical Research Development Unit, Shohada Hospital, Tabriz University of Medical Sciences, Tabriz, Iran and Cellular and Molecular Research Centre, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran. The authors report no conflicts of interest.
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