Gentamicin-Loaded Polysaccharide Membranes for Prevention and Treatment of Post-operative Wound Infections in the Skeletal System
- 340 Downloads
To develop polysaccharide-based membranes that allow controlled and localized delivery of gentamicin for the treatment of post-operative bone infections.
Membranes made of gellan gum (GUM), sodium alginate (ALG), GUM and ALG crosslinked with calcium ions (GUM + Ca and ALG + Ca, respectively) as well as reference collagen (COL) were produced by freeze-drying. Mechanical properties, drug release, antimicrobial activity and cytocompatibility of the membranes were assessed.
The most appropriate handling and mechanical properties (Young’s modulus, E = 92 ± 4 MPa and breaking force, F MAX = 2.6 ± 0.1 N) had GUM + Ca membrane. In contrast, COL membrane showed F MAX = 0.14 ± 0.02 N, E = 1.0 ± 0.3 MPa and was deemed to be unsuitable for antibiotic delivery. The pharmacokinetic data demonstrated a uniform and sustainable delivery of gentamicin from GUM + Ca (44.4 ± 1.3% within 3 weeks), while for COL, ALG and ALG + Ca membranes the most of the drug was released within 24 h (55.3 ± 1.9%, 52.5 ± 1.5% and 37.5 ± 1.8%, respectively). Antimicrobial activity against S. aureus and S. epidermidis was confirmed for all the membranes. GUM + Ca and COL membranes supported osteoblasts growth, whereas on ALG and ALG + Ca membranes cell growth was reduced.
GUM + Ca membrane holds promise for effective treatment of bone infections thanks to favorable pharmacokinetics, bactericidal activity, cytocompatibility and good mechanical properties.
Keywordsalginate gellan gum gentamicin local drug delivery membranes
Sodium alginate membrane
- ALG + Ca
Sodium alginate membrane crosslinked with calcium ions
Eagle’s minimal essential medium
European Committee on Antimicrobial Susceptibility Testing
Maximal elongation at break
Gellan gum membrane
- GUM + Ca
Gellan gum membrane crosslinked with calcium ions
Minimum inhibitory concentration
Osteosarcoma cell line
Molecular weight cut off
Scanning electron microscopy
Standard error of the mean
Tissue culture polystyrene
Ultra-high quality water
ACKNOWLEDGEMENTS AND DISCLOSURES
National Science Centre, Poland (Grant no: 012/05/B/ST8/00129) provided financial support to this project. The authors have no conflict of interest to declare.
- 15.Susheel C, Ramesh S, Chand SU, Ashwani S, Nitesh G, Daljit S, et al. Use of gentamicin-loaded collagen sponge in internal fixation of open fractures. Chin J Traumatol. 2011;14:209–14.Google Scholar
- 24.Wang HB, Zhou J. Injectable cardiac tissue engineering for the treatment of myocardial infarction. J Cell Mol Med. 2010;4:1044–55.Google Scholar
- 28.O’Neil MA, Silvendran RR, Morris J. Structure of extracellular gelling 566 polysaccharide produced by pseudomonas elodea. Curr Res. 1985;124:123–33.Google Scholar
- 41.The European Committee on Antimicrobial Susceptibility Testing, Version 5.0, 2015, http://www.eucast.org. Accessed 9 April 2016.
- 42.Włodarczyk-Biegun MK, Werten MWT, de Wolf FA, van den Beucken JJP, Leeuwenburgh SCG, Kamperman M, et al. Genetically engineered silk–collagen-like copolymer for biomedical applications: Production, characterization and evaluation of cellular response. Acta Biomater. 2014;10(8):3620–9.CrossRefPubMedGoogle Scholar