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Pharmaceutical Research

, Volume 34, Issue 9, pp 1773–1783 | Cite as

Polycaprolactone Based Nanoparticles Loaded with Indomethacin for Anti-Inflammatory Therapy: From Preparation to Ex Vivo Study

  • Waisudin Badri
  • Karim Miladi
  • Sophie Robin
  • Céline Viennet
  • Qand Agha Nazari
  • Géraldine Agusti
  • Hatem Fessi
  • Abdelhamid ElaissariEmail author
Research Paper

Abstract

Purpose

This work focused on the preparation of polycaprolactone based nanoparticles containing indomethacin to provide topical analgesic and anti-inflammatory effect for symptomatic treatment of inflammatory diseases. Indomethacin loaded nanoparticles are prepared for topical application to decrease indomethacin side effects and administration frequency. Oppositely to already reported works, in this research non-invasive method has been used for the enhancement of indomethacin dermal drug penetration. Ex-vivo skin penetration study was carried out on fresh human skin.

Methods

Nanoprecipitation was used to prepare nanoparticles. Nanoparticles were characterized using numerous techniques; dynamic light scattering, SEM, TEM, DSC and FTIR. Regarding ex-vivo skin penetration of nanoparticles, confocal laser scanning microscopy has been used.

Results

The results showed that NPs hydrodynamic size was between 220 to 245 nm and the zeta potential value ranges from −19 to −13 mV at pH 5 and 1 mM NaCl. The encapsulation efficiency was around 70% and the drug loading was about 14 to 17%. SEM and TEM images confirmed that the obtained nanoparticles were spherical with smooth surface. The prepared nanoparticles dispersions were stable for a period of 30 days under three temperatures of 4°C, 25°C and 40°C. In addition, CLSM images proved that obtained NPs can penetrate the skin as well.

Conclusion

The prepared nanoparticles are submicron in nature, with good colloidal stability and penetrate the stratum corneum layer of the skin. This formulation potentiates IND skin penetration and as a promising strategy would be able to decline the side effects of IND.

KEY WORDS

anti-inflammatory indomethacin nanoparticles polycaprolactone skin penetration 

ABBREVIATIONS

COX enzyme

Cyclooxygenase enzyme

CLSM

Confocal laser scanning microscopy

DSC

Differential scanning calorimeter

EE

Encapsulation efficiency

FTIR

Fourier transform infrared spectroscopy

IND

Indomethacin

NPs

Nanoparticles

NSAIDs

Non-Steroidal Anti-inflammatory Drugs

PCL

Polycaprolactone

PVA

Polyvinyl alcohol

RT

Room temperature (25°C)

SEM

Scanning Electron Microscopy

TEM

Transmission Electron Microscopy

Notes

Acknowledgments and Disclosures

Waisudin Badri gratefully acknowledges French foreign affairs ministry and Afghanistan higher education ministry for providing the PhD scholarship.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Waisudin Badri
    • 1
    • 2
  • Karim Miladi
    • 1
  • Sophie Robin
    • 3
  • Céline Viennet
    • 3
  • Qand Agha Nazari
    • 2
  • Géraldine Agusti
    • 1
  • Hatem Fessi
    • 1
  • Abdelhamid Elaissari
    • 1
    Email author
  1. 1.Univ Lyon, University Claude Bernard Lyon-1, CNRS, LAGEP UMR 5007VilleurbanneFrance
  2. 2.Faculty of PharmacyKabul UniversityKabulAfghanistan
  3. 3.Laboratory of Engineering and Cutaneous Biology, UMR 1098Bourgogne Franche-Comté UniversityBesançonFrance

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