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Pharmaceutical Research

, Volume 34, Issue 5, pp 1093–1103 | Cite as

Versatile Methodology to Encapsulate Gold Nanoparticles in PLGA Nanoparticles Obtained by Nano-Emulsion Templating

  • Cristina FornagueraEmail author
  • Natàlia Feiner-Gracia
  • Aurora Dols-Perez
  • Maria José García-Celma
  • Conxita Solans
Research Paper

Abstract

Purpose

Gold nanoparticles have been proved useful for many biomedical applications, specifically, for their use as advanced imaging systems. However, they usually present problems related with stability and toxicity.

Methods

In the present work, gold-nanoparticles have been encapsulated in polymeric nanoparticles using a novel methodology based on nano-emulsion templating. Firstly, gold nanoparticles have been transferred from water to ethyl acetate, a solvent classified as class III by the NIH guidelines (low toxic potential). Next, the formation of nano-emulsions loaded with gold nanoparticles has been performed using a low-energy, the phase inversion composition (PIC) emulsification method, followed by solvent evaporation giving rise to polymeric nanoparticles.

Results

Using this methodology, high concentrations of gold nanoparticles (>100 pM) have been encapsulated. Increasing gold nanoparticle concentration, nano-emulsion and nanoparticle sizes increase, resulting in a decrease on the stability. It is noteworthy that the designed nanoparticles did not produce cytotoxicity neither hemolysis at the required concentration.

Conclusions

Therefore, it can be concluded that a novel and very versatile methodology has been developed for the production of polymeric nanoparticles loaded with gold nanoparticles.

Graphical Abstract

Schematic representation of AuNP-loaded polymeric nanoparticles preparation from nano-emulsion templating

KEY WORDS

AuNP-loaded polymeric nanoparticles nano-emulsion templating novel imaging systems phase inversion composition (PIC) emulsification method) polymeric nanoparticles 

ABBREVIATIONS

AuNP

Gold nanoparticles

CTAB

Cetyltrimethylammonium bromide

DLS

Dynamic light scattering

NE(Au)

AuNP-loaded nano-emulsions

NP(Au)

AuNP-loaded PLGA nanoparticles

PIC

Phase inversion composition method

PLGA

Poly(lactide-co-glycolic acid)

RES

Reticuloendothelial system

TOAB

Tetraoctylammonium bromide

Notes

ACKNOWLEDGMENTS AND DISCLOSURES

Financial support from MINECO (grants CTQ2014-52687-C3-1-P and CTQ2016-80645-R); Generalitat de Catalunya (grant 2014-SGR-1655), and CIBER-BBN are acknowledged. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. Cristina Fornaguera is grateful to AGAUR for their Predoctoral Fellowship (grant FI-DGR 2012). Natàlia Feiner is grateful to CIBER-BBN for their Research Initiation Fellowship.

Supplementary material

11095_2017_2119_MOESM1_ESM.docx (197 kb)
ESM 1 (DOCX 196 kb)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Institute of Advanced Chemistry of Catalonia (IQAC-CSIC)BarcelonaSpain
  2. 2.CIBER of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)BarcelonaSpain
  3. 3.Department of Pharmacy and Pharmaceutical TechnologyUniversity of BarcelonaBarcelonaSpain

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