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Pharmaceutical Research

, Volume 30, Issue 7, pp 1735–1748 | Cite as

Electrospun Rapamycin-Eluting Polyurethane Fibers for Vascular Grafts

  • Jingjia Han
  • Shady Farah
  • Abraham J. Domb
  • Peter I. Lelkes
Research Paper

ABSTRACT

Purpose

To develop rapamycin-eluting electrospun polyurethane (PU) vascular grafts that could effectively suppress local smooth muscle cell (SMC) proliferation.

Methods

Rapamycin (RM) was incorporated in PU fibers by blend electrospinning using three distinct blending methods. The drug release profiles and the bioavailability of RM-containing PU fibers in the form of fibrous mats and vascular grafts were evaluated up to 77 days in vitro.

Results

RM-contained PU fibers generated by the three distinct blending methods exhibited significantly different fiber diameters (200–500 nm) and distinct RM release kinetics. Young’s moduli of the electrospun fibrous mats increased with higher RM contents and decreased with larger fiber diameters. For all blending methods, RM release kinetics was characteristic of a Fickian diffusion for at least 77 days in vitro. RM-PU fibers generated via powder blending showed the highest encapsulation efficiency. The RM in grafts made of these fibers remained bioactive and was still able to inhibit smooth muscle cell proliferation after 77 days of continual in vitro release.

Conclusions

Electrospun RM-containing PU fibers can serve as effective drug carriers for the local suppression of SMC proliferation and could be used as RM-eluting scaffolds for vascular grafts.

KEY WORDS

drug release electrospinning rapamycin restenosis smooth muscle cell 

ABBREVIATIONS

AB

almarBlue

BSA

bovine serum albumin

DMEM

Dulbecco’s Modified of Eagle’s Medium

FBS

fetal bovine serum

HFP-1

1, 1, 3, 3, 3-Hexafluoro-2-Propanol

HPLC

high performance liquid chromatography

NS-IP

normal saline-isopropyl alcohol solution

PANi

polyaniline

PBS

phosphate buffered saline

PGE

PLGA-gelatin-elastin

PLGA

poly(lactic-co-glycolic acid)

PTCA

percutaneous transluminal coronary angioplasty

PU

polyurethane

RM

rapamycin

SMC

smooth muscle cell

SPU

segmented polyurethane

TCP

tissue culture polystyrene

Notes

ACKNOWLEDGEMENTS AND DISCLOSURES

Jingjia Han and Shady Farah equally contributed to this paper. This work was supported by a translational research grant by HUB (DU/BIOMED-IDR/HUJI) from Drexel University and The Hebrew University of Jerusalem. We thank Dr. Gozde Senel-Ayaz (Drexel BIOMED) for her assistance with SEM and Dr. Wahid Khan (IDR) for his assistance with developing the analytical method used to assess drug release.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.School of Biomedical Engineering, Science and Health SystemsDrexel UniversityPhiladelphiaUSA
  2. 2.School of Pharmacy, Faculty of MedicineThe Hebrew University of JerusalemJerusalemIsrael
  3. 3.School of Pharmacy, Faculty of Medicine Center for Nanosciences and Nanotechnology Grass Center for Drug Design and SynthesisThe Hebrew University of JerusalemJerusalemIsrael
  4. 4.Department of Bioengineering, College of EngineeringTemple UniversityPhiladelphiaUSA

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