Pharmaceutical Research

, Volume 29, Issue 9, pp 2389–2397 | Cite as

Novel Imaging Method to Quantify Stratum Corneum in Dermatopharmacokinetic Studies

  • Lisa M. Russell
  • Richard H. Guy
Research Paper



Tape stripping the stratum corneum (SC) is used in topical bioequivalence studies. Formulations are compared using drug concentration profiles as a function of relative SC position; both of these parameters require quantification of SC amount removed per tape. Here, a novel imaging method to quantify SC on tape strips is described. Comparisons are made with established SC quantification methods, specifically weighing and UV pseudo-absorption.


Six stratum corneum tape strips were measured 15 times by the three methods, which were compared for precision, signal:noise ratio, sample size and speed. Furthermore, 600 tape strips were assayed by each method, and correlations examined.


Weighing exhibited low precision, extremely low signal:noise ratio, and was slow. UV pseudo-absorption had high precision, acceptable signal:noise ratio and was quick. However, only a fraction of the total SC removed is analysed, and inhomogeneity can affect the results. The new imaging method was precise, with high signal:noise ratio, and measured the whole SC sample, unaffected by inhomogeneity. In addition, the approach was rapid and has the potential for fast automated scanning of multiple tapes and for further image analysis.


The novel imaging method has many advantages over established methods for quantifying SC amount per tape.

Key Words

dermatopharmacokinetics imaging stratum corneum tape stripping 





stratum corneum


transepidermal water loss


Acknowledgments and Disclosures

We thank many colleagues for helpful comments and suggestions on the manuscript, and the University of Bath for a studentship (LMR).

Supplementary material

11095_2012_764_MOESM1_ESM.doc (34 kb)
Esm 1 (DOC 33 kb)


  1. 1.
    Alberti I, Kalia YN, Naik A, Guy RH. Assessment and prediction of the cutaneous bioavailability of topical terbinafine, in vivo, in man. Pharm Res. 2001;18:1472–5.PubMedCrossRefGoogle Scholar
  2. 2.
    Kalia YN, Pirot F, Guy RH. Homogeneous transport in a heterogeneous membrane: water diffusion across human stratum corneum in vivo. Biophys J. 1996;71:2692–700.PubMedCrossRefGoogle Scholar
  3. 3.
    Russell LM, Wiedersberg S, Delgado-Charro MB. The determination of SC thickness—an alternative approach. Eur J Pharm Biopharm. 2008;69:861–70.PubMedCrossRefGoogle Scholar
  4. 4.
    Herkenne C, Naik A, Kalia YN, Hadgraft J, Guy RH. Pig ear skin ex vivo as a model for in vivo dermatopharmacokinetic studies in man. Pharm Res. 2006;23:1850–6.PubMedCrossRefGoogle Scholar
  5. 5.
    Wiedersberg S, Leopold CS, Guy RH. Bioavailability and bioequivalence of topical glucocorticoids. Eur J Pharm Biopharm. 2008;68:453–66.PubMedCrossRefGoogle Scholar
  6. 6.
    Alberti I, Kalia YN, Naik A, Bonny JD, Guy RH. In vivo assessment of enhanced topical delivery of terbinafine to human stratum corneum. J Control Release. 2001;71:319–27.PubMedCrossRefGoogle Scholar
  7. 7.
    Wiedersberg S, Leopold CS, Guy RH. Dermatopharmacokinetics of betamethasone 17-valerate: Influence of formulation viscosity and skin surface cleaning procedure. Eur J Pharm Biopharm. 2009;71:362–6.PubMedCrossRefGoogle Scholar
  8. 8.
    Herkenne C, Naik A, Kalia YN, Hadgraft J, Guy RH. Dermatopharmacokinetic prediction of topical drug bioavailability in vivo. J Invest Dermatol. 2007;127:887–94.PubMedCrossRefGoogle Scholar
  9. 9.
    Herkenne C, Naik A, Kalia YN, Hadgraft J, Guy RH. Ibuprofen transport into and through skin from topical formulations: in vitro-in vivo comparison. J Invest Dermatol. 2007;127:135–42.PubMedCrossRefGoogle Scholar
  10. 10.
    Herkenne C, Naik A, Kalia YN, Hadgraft J, Guy RH. Effect of propylene glycol on ibuprofen absorption into human skin in vivo. J Pharm Sci. 2008;97:185–97.PubMedCrossRefGoogle Scholar
  11. 11.
    Alberti I, Kalia YN, Naik A, Bonny J, Guy RH. Effect of ethanol and isopropyl myristate on the availability of topical terbinafine in human stratum corneum, in vivo. Int J Pharm. 2001;219:11–9.PubMedCrossRefGoogle Scholar
  12. 12.
    Kalia YN, Alberti I, Sekkat N, Curdy C, Naik A, Guy RH. Normalization of stratum corneum barrier function and transepidermal water loss in vivo. Pharm Res. 2000;17:1148–50.PubMedCrossRefGoogle Scholar
  13. 13.
    Wiedersberg S, Naik A, Leopold CS, Guy RH. Pharmacodynamics and dermatopharmacokinetics of betamethasone 17-valerate: assessment of topical bioavailability. Br J Dermatol. 2009;160:676–86.PubMedCrossRefGoogle Scholar
  14. 14.
    Nicoli S, Bunge AL, Delgado-Charro MB, Guy RH. Dermatopharmacokinetics: factors influencing drug clearance from the stratum corneum. Pharm Res. 2009;26:865–71.PubMedCrossRefGoogle Scholar
  15. 15.
    Weigmann HJ, Lindemann U, Antoniou C, Tsikrikas GN, Stratigos AI, Katsambas A, Sterry W, Lademann J. UV/VIS absorbance allows rapid, accurate, and reproducible mass determination of corneocytes removed by tape stripping. Skin Pharmacol Appl Skin Physiol. 2003;16:217–27.PubMedGoogle Scholar
  16. 16.
    Weigmann HJ, Lademann J, Meffert H, Schaefer H, Sterry W. Determination of the horny layer profile by tape stripping in combination with optical spectroscopy in the visible range as a prerequisite to quantify percutaneous absorption. Skin Pharmacol Appl Skin Physiol. 1999;12:34–45.PubMedGoogle Scholar
  17. 17.
    Dreher F, Arens A, Hostynek JJ, Mudumba S, Ademola J, Maibach HI. Colorimetric method for quantifying human Stratum corneum removed by adhesive-tape stripping. Acta Derm Venereol. 1998;78:186–9.PubMedCrossRefGoogle Scholar
  18. 18.
    Dreher F, Modjtahedi BS, Modjtahedi SP, Maibach HI. Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Res Technol. 2005;11:97–101.PubMedCrossRefGoogle Scholar
  19. 19.
    Chaoand YC, Nylander-French LA. Determination of keratin protein in a tape-stripped skin sample from jet fuel exposed skin. Ann Occup Hyg. 2004;48:65–73.CrossRefGoogle Scholar
  20. 20.
    Marttin E, Neelissen-Subnel MTA, De Haan FHN, Boddé HE. A critical comparison of methods to quantify stratum corneum removed by tape stripping. Skin Pharmacol. 1996;9:69–77.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Pharmacy & PharmacologyUniversity of BathBathUK

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