Pharmaceutical Research

, Volume 29, Issue 2, pp 362–374

Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles

  • Won Ho Kong
  • Ki Hyun Bae
  • Sung Duk Jo
  • Jee Seon Kim
  • Tae Gwan Park
Research Paper

DOI: 10.1007/s11095-011-0554-y

Cite this article as:
Kong, W.H., Bae, K.H., Jo, S.D. et al. Pharm Res (2012) 29: 362. doi:10.1007/s11095-011-0554-y

ABSTRACT

Purpose

Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.

Methods

Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.

Results

Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.

Conclusions

We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.

KEY WORDS

cancer therapy cationic lipid delivery system gold nanoparticle siRNA 

Supplementary material

11095_2011_554_MOESM1_ESM.pdf (112 kb)
Supplemental Figure 1(PDF 111 kb)
11095_2011_554_MOESM2_ESM.pdf (82 kb)
Supplemental Figure 2(PDF 82.3 kb)
11095_2011_554_MOESM3_ESM.pdf (113 kb)
Supplemental Figure 3(PDF 113 kb)
11095_2011_554_MOESM4_ESM.pdf (121 kb)
Supplemental Figure 4(PDF 120 kb)

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Won Ho Kong
    • 1
    • 2
  • Ki Hyun Bae
    • 1
  • Sung Duk Jo
    • 1
  • Jee Seon Kim
    • 1
  • Tae Gwan Park
    • 1
  1. 1.Department of Biological SciencesGraduate School of Nanoscience & Technology Korea Advanced Institute of Science & TechnologyDaejeonSouth Korea
  2. 2.Department of Material Science & EngineeringPohang University of Science & TechnologyPohangSouth Korea

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