Pharmaceutical Research

, 28:1653 | Cite as

Antitumour Efficacy of Two Paclitaxel Formulations for Hyperthermic Intraperitoneal Chemotherapy (HIPEC) in an In Vivo Rat Model

  • Wim Bouquet
  • Steven Deleye
  • Steven Staelens
  • Lieselotte De Smet
  • Nancy Van Damme
  • Isabelle Debergh
  • Wim P. Ceelen
  • Filip De Vos
  • Jean Paul Remon
  • Chris Vervaet
Research Paper

ABSTRACT

Purpose

To evaluate the tumour growth delay of a peritoneal carcinomatosis (PC) of colorectal origin after intraperitoneal chemotherapy with paclitaxel/randomly-methylated-β-cyclodextrin (Pac/RAME-β-CD) versus Taxol® at normo- and hyperthermic conditions in rats.

Methods

Hyperthermic intraperitoneal chemotherapy (HIPEC) was performed 7 days post implantation of the tumour with both formulations at a Pac concentration of 0.24 mg/ml. Tumour evaluation was performed via positron emission tomography (PET) and magnetic resonance imaging (MRI) imaging, measuring tumour activity and tumour volume, respectively. Scans were taken at 2 and 7 days post treatment.

Results

PET and MRI data showed a significant reduction in tumour activity and tumour volume for rats treated with Pac/RAME-β-CD (at normo- and hyperthermic conditions), compared to the control group. Treatment with Taxol® did not result in a significant reduction of tumour activity and tumour volume. No significant differences between the normo- and hyperthermic conditions were observed for both formulations, indicating that hyperthermia and paclitaxel were not synergistic despite the direct cytotoxic effect of hyperthermia.

Conclusion

Monitoring tumour growth via PET and MRI indicated that Pac/RAME-β-CD inclusion complexes had a significantly higher efficacy compared to Taxol® in a rat model for peritoneal carcinomatosis.

KEY WORDS

hyperthermic intraperitoneal chemotherapy paclitaxel tumour growth delay β-cyclodextrin 

Supplementary material

11095_2011_401_MOESM1_ESM.docx (465 kb)
Illustration 1Example of a μPET image at day 6, maximum tumor to liver ratio of 3.4 (arrow indicates tumour) (DOCX 465 kb)
11095_2011_401_MOESM2_ESM.docx (121 kb)
Illustration 2Example of μMRI image at day 6, volume determination results in 0,13 cm3 (arrow indicates tumour) (DOCX 121 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Wim Bouquet
    • 1
  • Steven Deleye
    • 2
  • Steven Staelens
    • 2
    • 3
  • Lieselotte De Smet
    • 1
  • Nancy Van Damme
    • 4
  • Isabelle Debergh
    • 5
  • Wim P. Ceelen
    • 5
  • Filip De Vos
    • 6
  • Jean Paul Remon
    • 1
  • Chris Vervaet
    • 1
  1. 1.Laboratory of Pharmaceutical TechnologyGhent UniversityGhentBelgium
  2. 2.Medical Image and Signal ProcessingGhent University-IBBTGhentBelgium
  3. 3.Molecular Imaging Center AntwerpAntwerp UniversityAntwerpBelgium
  4. 4.Department of GastroenterologyGhent University HospitalGhentBelgium
  5. 5.Department of Gastrointestinal SurgeryGhent University HospitalGhentBelgium
  6. 6.Laboratory of RadiopharmacyGhent UniversityGhentBelgium

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