An Effective Strategy for the Synthesis of Biocompatible Gold Nanoparticles Using Cinnamon Phytochemicals for Phantom CT Imaging and Photoacoustic Detection of Cancerous Cells
The purpose of the present study was to explore the utilization of cinnamon-coated gold nanoparticles (Cin-AuNPs) as CT/optical contrast-enhancement agents for detection of cancer cells.
Cin-AuNPs were synthesized by a “green” procedure, and the detailed characterization was performed by physico-chemical analysis. Cytotoxicity and cellular uptake studies were carried out in normal human fibroblast and cancerous (PC-3 and MCF-7) cells, respectively. The efficacy of detecting cancerous cells was monitored using a photoacoustic technique. In vivo biodistribution was studied after IV injection of Cin-AuNPs in mice, and also a CT phantom model was generated.
Biocompatible Cin-AuNPs were synthesized with high purity. Significant uptake of these gold nanoparticles was observed in PC-3 and MCF-7 cells. Cin-AuNPs internalized in cancerous cells facilitated detectable photoacoustic signals. In vivo biodistribution in normal mice showed steady accumulation of gold nanoparticles in lungs and rapid clearance from blood. Quantitative analysis of CT values in phantom model revealed that the cinnamon-phytochemical-coated AuNPs have reasonable attenuation efficiency.
The results indicate that these non-toxic Cin-AuNPs can serve as excellent CT/ photoacoustic contrast-enhancement agents and may provide a novel approach toward tumor detection through nanopharmaceuticals.
KEY WORDScancer cells cellular internalization cinnamon-stabilized gold nanoparticles in vivo biodistribution phantom CT imaging photoacoustic detection
- 6.Feitz A, Guan J, Waite D. Process for producing a nanoscale zero-valent metal. US Patent Application Publication. US2006/0083924 A1 (2006).Google Scholar
- 7.Fent GM, Casteel SW, Kim DY, Kannan R, Katti K, Chanda N. Biodistribution of maltose and gum arabic hybrid gold nanoparticles after intravenous injection in juvenile swine. Nanomedicine:NBM. 2009;5:128–35.Google Scholar
- 14.Shukla R, Nune SK, Chanda N, Katti K, Mekapothula S, Kulkarni RR, et al. Soybeans as a phytochemical reservoir for the production and stabilization of biocompatible gold nanoparticles. Science Editors’ Choice. 2008;322:167.Google Scholar
- 19.Gow RT, Li D, Sypert GW, Alberte RS. Extracts and methods comprising cinnamon species. US Patent Application Publication US 2007/0292540 Al (2007).Google Scholar
- 26.Faha OR. OSIRIX: An oepn source platform for advanced multimodality medical imaging. IEEE Information & Communications Technology (2006).Google Scholar
- 29.Kannan R, Cutler C, Rahing V, Smith C, Katti K. Bioconjugated radioactive gold nanoparticles and their in vivo targeting abilities in small animal models. J Nucl Med. 2006;510(Supplement 1):510.Google Scholar
- 30.Kannan R, Katti KV, Katti KK, White HW, Cutler CS. Methods and articles for gold nanoparticle production. US 2007/0051202 A1, US Patent Application Publication (2007).Google Scholar
- 31.Katti K, Kannan R, Katti KK, Bhaskaran S, Pandrapragada RK. Optimization and production of gold and silver nanoparticles for potential imaging applications. Molecular Imaging. 2004;3:278.Google Scholar
- 32.Chanda N, Kan P, Watkinson LD, Shukla R, Zambre A, Carmack TL, et al. Radioactive gold nanoparticles in cancer therapy: therapeutic efficacy studies of 198AuNP-GA nanoconstruct in prostate tumor bearing mice. Nanomedicine: NBM. 2009;6:201–9.Google Scholar
- 37.Mallidi S, Joshi PP, Sokolov K, Emelianov S. On sensitivity of molecular specific photoacoustic imaging using plasmonic gold nanoparticles. Conf Proc IEEE Eng Med Biol Soc. 6338–6340 (2009).Google Scholar