Pharmaceutical Research

, Volume 27, Issue 11, pp 2283–2295 | Cite as

PEG-Functionalized Magnetic Nanoparticles for Drug Delivery and Magnetic Resonance Imaging Applications

  • Murali Mohan Yallapu
  • Susan P. Foy
  • Tapan K. Jain
  • Vinod Labhasetwar
Research Paper

ABSTRACT

Purpose

Polyethylene glycol (PEG) functionalized magnetic nanoparticles (MNPs) were tested as a drug carrier system, as a magnetic resonance imaging (MRI) agent, and for their ability to conjugate to an antibody.

Methods

An iron oxide core coated with oleic acid (OA) and then with OA-PEG forms a water-dispersible MNP formulation. Hydrophobic doxorubicin partitions into the OA layer for sustained drug delivery. The T1 and T2 MRI contrast properties were determined in vitro and the circulation of the MNPs was measured in mouse carotid arteries. An N-hydroxysuccinimide group (NHS) on the OA-PEG-80 was used to conjugate the amine functional group on antibodies for active targeting in the human MCF-7 breast cancer cell line.

Results

The optimized formulation had a mean hydrodynamic diameter of 184 nm with an ~8 nm iron-oxide core. The MNPs enhance the T2 MRI contrast and have a long circulation time in vivo with 30% relative concentration 50 min post-injection. Doxorubicin-loaded MNPs showed sustained drug release and dose-dependent antiproliferative effects in vitro; the drug effect was enhanced with transferrin antibody-conjugated MNPs.

Conclusion

PEG-functionalized MNPs could be developed as a targeted drug delivery system and MRI contrast agent.

KEY WORDS

anticancer drugs anti-proliferative effect contrast agent iron-oxide reticuloendothelial system transferrin antibody 

Notes

ACKNOWLEDGMENTS

The study reported here is funded by grant R01 EB005822 (to VL) from the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health. SPF is a predoctoral student in Cleveland Clinic’s Molecular Medicine Ph.D. Program, which is funded by the “Med into Grad” initiative of the Howard Hughes Medical Institute (http://www.lerner.ccf.org/molecmed/phd/).

Supplementary material

11095_2010_260_MOESM1_ESM.doc (96 kb)
Supplementary materials(DOC 96 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Murali Mohan Yallapu
    • 1
    • 2
  • Susan P. Foy
    • 1
  • Tapan K. Jain
    • 1
    • 3
  • Vinod Labhasetwar
    • 1
    • 4
  1. 1.Department of Biomedical Engineering/ND-20 Lerner Research InstituteCleveland ClinicClevelandUSA
  2. 2.Cancer Biology Research CenterSanford Research/University of South DakotaSioux FallsUSA
  3. 3.University School of Basic & Applied SciencesGuru Gobind Singh Indraprastha UniversityNew DelhiIndia
  4. 4.Taussig Cancer CenterCleveland ClinicClevelandUSA

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