Identification of Peptide Ligands for Targeting to the Blood-Brain Barrier
Transport of drugs to the brain is limited by the blood-brain barrier. New, specific brain endothelium ligands can facilitate brain-specific delivery of drugs.
We used phage display in an in situ brain perfusion model to screen for new brain endothelium peptide ligands.
Two phage clones, displaying 15 amino acid-peptides (GLA and GYR) that were selected for brain binding in the mouse model, showed significant binding to human brain endothelium (hCMEC/D3), compared to a random control phage. This binding was not seen for other human endothelial cells (HUVEC). Binding to hCMEC/D3 cells was dose dependent. When phage GLA and GYR were individually perfused through the murine brain, their ability to bind to the brain was 6-fold (GLA) and 5-fold (GYR) higher than the control phage. When compared to lung perfusion, phage showed an 8.5-fold (GYR) and 48-fold (GLA) preference for brain over lung compared to the control.
These results indicate that two new peptide ligands have been identified that may be used for specific targeting of drugs to the blood-brain barrier.
KEY WORDSblood-brain barrier brain targeting peptide ligand phage display targeting ligand
Transport of drugs to the brain is limited by the presence of the blood-brain barrier (BBB). This barrier is formed by specialized endothelial cells and supported by other cell types, such as astrocytes and pericytes (1). The BBB regulates homeostasis of the brain and is selectively permeable for the uptake and efflux of ions, nutrients and metabolites (2). Drug transport via the paracellular route is prevented by tight junctions between the endothelial cells. Free diffusion via the transcellular route is accessible only to lipophilic compounds smaller than approximately 400 Da (3). This limits the treatment of many brain diseases, such as Alzheimer’s disease and Parkinson’s disease. Even in certain pathological situations where the BBB is partly disrupted, drug transport remains limited (4).
To overcome this limitation, carrier systems, such as nanoparticles, have been used to deliver drugs to the brain. Antibody ligands have been successfully coupled to the nanoparticles to target the brain endothelial cell receptors (5,6). The main receptors that have been targeted are the transferrin receptor (7) and the insulin receptor (8). However, these receptors are not brain specific. They are widely expressed in peripheral organs (9), limiting their selectivity and applicability as brain-targeting receptors. Therefore, identification of new brain targets is needed to deliver drugs more selectively into the brain. The objective of this study was to identify new targeting ligands that can be used for active targeting of drug delivery systems to the brain, aiming for selectivity for brain endothelium.
Peptides are highly suitable for ligand screening, when expressed on a phage display system, and they have been widely used as targeting ligands (10). Phage display is a powerful technology for ligand identification (11, 12, 13, 14). Large libraries expressing peptides or proteins can be screened for target affinity (15). We used a random 15-mer peptide library to identify brain-targeting peptide ligands. In vivo, blood-brain barrier endothelial cells get stimulated by their surrounding cells (16,17) and intraluminal blood flow (18). This contributes to the complexity of the blood-brain barrier and regulates the expression of specific receptors at the cell surface in a polarized fashion. For this reason, ligand screening should preferably be performed in vivo. However, as in vivo phage screening in humans was impossible for ethical reasons, we chose to pre-select phage in a mouse perfusion model to enrich for peptides recognizing brain endothelial cells in their natural environment. Because the brain remains intact, the endothelial cells keep their polarity and their contact with the surrounding cells, maintaining expression of relevant receptors. After the pre-selection for in vivo relevant receptors, identified ligands were tested for cross-reactivity with human cell-surface receptors expressed by human endothelial cells in vitro. In this way, the complexity of the in vivo BBB and the significance of the human BBB were combined.
MATERIALS AND METHODS
Phage Library and Bacteria
Filamentous phage fd-tet, which confers tetracycline resistance on the host (19), was used for this study. Random peptide library f3-15mer (20), containing 2.5 × 108 primary clones (GenBank Accession AF246445) with foreign 15-mer peptide displayed on pIII was provided by George P. Smith (Columbia, MO). Kanamycin resistant E. coli K91BluKan (K91BK) host bacteria were also provided by George P. Smith. The K91BK sex is Hfr Cavalli, resulting in deployment of the F pilus, the attachment site for filamentous phage infection.
In situ phage display screening was performed in male, 6–8 week-old C57Bl/6 mice (Charles River, The Netherlands). Perfusion of single clones was done in male 28–32 g Balb/c mice (Harlan, Horst, The Netherlands). Food and water were supplied ad libitum. Animal studies were performed according to national regulations and were approved by the local animal experiments ethical committee.
In Situ Brain Perfusion of Phage
Forty units of heparin (Sigma) were injected i.v. into the tail vein to prevent coagulation of blood inside the microvessels (21). Five minutes after injection, mice were sacrificed by CO2 asphyxiation. Next, thorax was opened, and bulldog clamps with 8 × 1.2 mm serrated jaws (WPI, Berlin, Germany) were placed on descending aorta (left from the carotid arteries) and pulmonary veins and arteries. Inferior caval vein was cut to allow outflow of perfused fluids. A 26G needle, connected to a peristaltic pump, was inserted into the left ventricle of the heart (22). Mice were initially perfused with 1.5 ml Hanks’ Balanced Salt Solution (HBSS, pH 7.0–7.4, Invitrogen, Carlsbad, CA) in all experiments. Directly afterwards, mice were perfused with phage. Upon the three selection rounds, 1 ml of phage library in HBSS (8.9 × 1011 TU, 3.1 × 1011 TU, and 4.8 × 1010 TU for the first, second and third selection rounds, respectively) were perfused (input). To wash away non-binders, 3.5 ml HBSS was perfused directly afterwards. For the third selection round, HBSS + 1% FBS was used instead of HBSS. For perfusions of selected phage (single clones), 750 µl of phage (∼1010 TU) in HBSS + 1% FBS was perfused, directly followed by 2 ml of HBSS + 1% serum to wash. Peristaltic pump speed was 200 μl/min. Directly after perfusion, brains were taken out. For the phage display screening, phage were isolated from the brain (see below).
Phage Isolation from Brain
After phage display selection rounds, brain cerebrum was ground in 600 μl 100 mM triethanolamine (Fluka, Munich, Germany) and centrifuged for 10 min at 3300 ×g. Supernatant was transferred to a new tube, neutralized with 300 μl 1 M Tris pH 7.4, and centrifuged for 5 min at 3300 ×g. Late log phase K91BK were obtained by inoculation of 10 ml of terrific broth with 100 μl of an overnight culture, and incubation for 4 h in a shaker incubator (250 rpm, 37°C). When late log phase was reached (when the OD600 of a 10× dilution reached 0.2 on a spectrophotometer), shaking was slowed down to 75 rpm for 5 min to allow sheared F pili to regenerate. Brain supernatant was incubated with late log phase K91BK for 10 min to allow phage to infect the bacteria. The infected bacteria were added to 40 ml LB Broth (Sigma) containing 0.2 μg/ml tetracycline and amplified for 30 min in a shaker incubator (250 rpm, 37°C). Two hundred μl of the infected K91BK were spread on LB agar plates for titration. Tetracycline concentration was then brought to 20 μg/ml, and phage were amplified overnight in the shaker incubator. Cultures were cleared of bacteria by two 10 min centrifugations at 3000 and 8000 ×g. To the cleared culture 0.15 volume PEG/NaCl (16.7% /3.3 M stock) was added (PEG 8000, Promega, Breda, The Netherlands). Phage were precipitated overnight at 4°C. Precipitated phage were collected by centrifugation for 15 min at 17500 ×g at 4°C. Pellets were dissolved in 1 ml TBS and precipitated again with 0.15 vol PEG/NaCl for at least 1 h at 4°C. Phage were collected by centrifugation for 5 min at 16000 ×g. Pellets were dissolved in 400 μl TBS and stored at 4°C. Two hundred μl of purified phage + 800 μl HBSS were used for the next selection round. Three successive selection rounds were done in total. After the third selection round, brain cerebrum was separated into anterior and posterior part. Phage were separately isolated from both parts. Titration of input phage was performed as described below. After perfusion of single phage clones, whole brains were ground in 1,000 μl triethanolamine and centrifuged for 10 min at 3300 ×g. Two hundred μl supernatant was transferred to a new tube, and neutralized with 100 μl 1 M Tris pH 7.4. Titration of both input and output phage was performed as described below.
Titration of Phage
Transducing units (TU) were determined by titration. Phage were serially diluted in 0.1% (w/v) gelatin in TBS. Ten μl of phage dilution were incubated with 10 μl of late log phase K91BK for 10 min at room temperature. One ml LB, containing 0.2 μg/ml tetracycline, was added. This was incubated for 40 min in a shaker incubator (250 rpm, 37°C). Two-hundred μl of the infected K91BK were spread on LB agar plates containing 40 μg/ml tetracycline and 100 μg/ml kanamycin. Plates were incubated overnight at 37°C. The next day, the number of colonies was counted, and the number of transducing units was calculated. Input phage were titrated as well at the same time for recovery percentage calculations.
Immunohistochemistry Brain Cryosections
Frozen untreated brains were coated with OCT embedding matrix (Cellpath, Newtown, UK). Sections were cut along the sagittal plane using low-profile microtome blades (Leica, Heidelberg, Germany) on a CM3050 cryostat (Leica). Sections were transferred onto Superfrost Plus glass slides (Menzel, Germany). Section thickness was 5 μm. They were dried overnight at room temp and stored at −80°C. Upon use, sections were allowed to thaw for 20 min, fixed in acetone for 10 min, and air dried for 30 min. Separate sections were encircled with a liquid-repellent marker. All incubations were done in a moist environment, and incubation volumes were 90 μl/section. Non-specific binding sites and endogenous biotin were blocked simultaneously with avidin/biotin-blocking solution in PBS + 5% FCS (Vector Laboratories, Inc., Burlingame, USA), according to manufacturer’s instructions. Brain sections were incubated with either the enriched library or the unselected library (both 2.37 × 1013 V/ml) for 1 h at room temperature. Sections were incubated with 0.8 μg/ml biotinylated anti-phage antibody (Abcam, Cambridge, UK) for 1 h. Thereafter, sections were incubated with 5 μg/ml alexa568-conjugated streptavidin (Invitrogen) for 35 min. Sections were mounted in FluorSave (Calbiochem, San Diego, CA) and viewed under a Nikon Eclipse TE2000-U epi-fluorescence microscope (Nikon, Tokyo, Japan) at a 20× magnification.
Peptide sequences were aligned to check for peptides’ consensus sequences along the 15 amino acids. Software used was Vector NTI Advance 10 AlignX (Invitrogen).
Human brain endothelial cells (hCMEC/D3) were obtained from Institut National de la Santé et de la Recherche Médicale (INSERM, Paris, France). Cells were cultured at 37°C, 5% CO2, in EBM-2 basal medium supplemented with EGM-2 MV BulletKit (Lonza, Basel, Switzerland), containing growth factors and 2.5% FBS. Additionally, 10 mM HEPES, 1 ng/ml bFGF (Invitrogen), and antibiotics (Penicillin and Streptomycin) were added. hCMEC/D3 cells were grown on surfaces coated with 100 μg/ml Rat tail collagen type 1 (BD Biosciences). Prior to use, cell culture medium was replaced by cell differentiation medium, consisting of EBM-2 basal medium supplemented with 2.5% FBS, 1.4 μM hydrocortisone, 1 ng/ml bFGF, 10 mM HEPES, and antibiotics. Cells were grown to a monolayer in differentiation medium for one week. Medium was replaced every 2–3 days.
Human umbilical vein endothelial cells (HUVEC) were cultured at 37°C, 5% CO2, in EBM-2 basal medium supplemented with antibiotics and EGM-2 BulletKit (Lonza), containing growth factors and 2% FBS.
In Vitro Binding of Single Phage Clones
Cells were seeded at 3.4·105 cells/well in 12-well plates. Single phage clones were diluted to ∼1010 TU/ml in 25 mM HEPES in HBSS, and 1 ml phage solution was incubated with either hCMEC/D3 or HUVEC cells for 1 h at 37°C. The buffer containing unbound phage was removed, and cells were washed three times with PBS. Cells were washed three times with 1 ml of 0.2 M glycine (pH 2.2) to elute surface-bound phage (23,24). Three ml wash eluate was collected and neutralized with 0.45 ml 1 M tris (pH 9.1). Cells were washed again with 1 ml of PBS and were subsequently lysed with 0.25 ml 100 mM triethanolamine for 10 min (25,26). Cell lysates were collected and neutralized with 0.0625 ml 1 M tris (pH 7.4). The neutralized wash eluates (binding fraction) and cell lysates (strong binding/uptake fraction) were titrated as described above. Input phage were titrated as well at the same time for recovery percentage calculations.
Immunohistochemistry hCMEC/D3 Cells
hCMEC/D3 cells were grown to a monolayer on rat tail collagen coated Lab-Tek chamber slides (Nunc, Rochester, NY). Single phage clones were diluted to 5·1012 V/ml in HBSS, and 100 μl phage solution or 100 μl HBSS only was incubated with the cells for 1 h at 37°C. The buffer containing unbound phage was removed, and cells were washed three times with PBS. Cells were fixed in 4% formaldehyde for 30 min. Cells were washed two times with PBS and incubated with 0.8 μg/ml biotinylated anti-phage antibody for 1 h. Thereafter, cells were incubated with 5 μg/ml alexa568-conjugated streptavidin for 35 min, and washed three times. Cells were incubated with 300 nM DAPI (Invitrogen) for 2 min and washed three times with demi water. Cells were mounted in FluorSave and viewed under a Nikon Eclipse TE2000-U epi-fluorescence microscope at a 20× magnification.
In Situ Lung Perfusion of Phage
In situ lung perfusion was performed in the same way as was done for brain perfusions of single clones, with the only modification being the placement of the bulldog clamps. The ascending aorta was clamped, and phage were perfused through the left ventricle of the heart. Inferior caval vein was cut to allow outflow of perfused fluids. Phage isolation and titration were also performed in the same way as was done for the brain perfusions.
Data were analyzed using Graphpad Prism 4 for Windows software (Graphpad Software, San Diego, CA).
In situ phage display screening was used as a tool to search for new blood-brain-barrier-binding peptides. Screening was performed by perfusion of a 15 amino acid random peptide phage display library through the murine brain in situ. Phage perfusion (27,28) was chosen over intravenous injection, because clearance of phage occurs rapidly via the major organs of the reticuloendothelial system (mainly liver and spleen), resulting in a half-life of fd-tet of only 12 min (29). Therefore, phage were directly perfused via the heart through the brain, preventing loss to other organs, and ensuring a high fraction of individual peptide-displaying phage to reach the target tissue.
Various in situ brain perfusion methods have been developed (30). Most models have been established in rats, but murine models have been used as well (22,31,32). Most perfusion models have been used to study brain uptake of substrates and were performed under anesthesia. Since filamentous phage are approximately 1 μm in size (33), they are not likely to be taken up into the brain, and the perfusion allows screening for binding rather than for internalizing peptides. Therefore, body temperature maintenance was not required, and perfusion was performed at room temperature, postmortem. The large majority of brain proteins are stable during a postmortem interval of up to 4 h at 25°C (34). As perfusions were completed within 30 min after sacrifice, the postmortem state was not expected to cause significant changes in brain microvessel morphology.
Phage were infused via the heart. The major advantage compared to direct infusion into the brain is that the phage have to travel some distance before they reach the brain. On their way, they already encounter endothelial cells: the endothelium of the aorta and carotid arteries. Phage with affinity to ubiquitous endothelial receptors are given the opportunity to bind to these cells and will not reach the brain. This negative selection filters out unspecific binders and allows for the identification of peptides that bind more specifically to brain endothelium (35,36).
Phage display screening was performed by perfusion of a mouse brain with 8.9 × 1011 Transducing Units (TU) in Hanks’ Balanced Salt Solution (HBSS), containing 1 g/L glucose, to keep the brain in a viable state. Phage were perfused for 5 min. Phage incubation times of 5–15 min are common and have been successful in vivo (11,37, 38, 39, 40). Non-binders were washed away by perfusion of HBSS. The brain was taken out, and phage were isolated from the cerebrum and submitted to the next selection round. During the third selection round, 1% FBS was added to the HBSS to limit unspecific interactions. Because the infused fluid was not evenly perfused throughout the brain (see supporting information paragraph), cerebrum was divided into its anterior (weakly perfused) and posterior (strongly perfused) side after the third selection round to investigate whether different ligands could be identified in the different brain parts.
Sequenced Phage Clones. Clones After 3 Selection Rounds, Isolated from Anterior (A) and Posterior (B) Cerebrum. Control Clones (C), Randomly Taken from Naive Library. Isoelectric Point (pI) and Charge at pH 7 Were Calculated by Vector NTI Advance 10 AlignX Software. Clones Tested In Vitro (y = yes) are Shown in Fig. 2
charge at pH 7
Tested in vitro
A. Clones isolated from anterior cerebrum
B. Clones isolated from posterior cerebrum
C. Random control clones
From both brain and lung recovery percentages, the preference ratio for brain over lung compared to RVR was calculated. Phage GYR showed a 8.5-fold preference, and phage GLA showed a 48-fold preference for brain over lung compared to the control, implying specific affinity of these phage-displayed peptides to brain endothelium.
Using the powerful technique of phage display, we identified two peptides that show significant binding to the brain compared to a control peptide. It is interesting to see that two peptides with opposite net charges both bind to the blood-brain barrier. Cell membranes are negatively charged (44). Therefore, positively charged molecules may bind to the cell surface non-specifically by electrostatic interactions (45). Selected peptide GYR and control peptide RVR are both positively charged (Table I), yet GYR shows a significantly higher binding to the brain than RVR. Furthermore, the negatively charged GLA shows significant binding to the brain. These results indicate that the binding of the selected peptides is dependent on more than electrostatic interactions with the endothelium.
When looking at the recovery from brain and lung, a notable difference in recovery percentages was evident. The base level of phage recovery from lung was higher than from brain. This can be explained by mainly two factors. First, traveling from heart to brain, the perfused fluid passes a longer distance compared to perfusion through the lung. Lungs are directly connected to the heart, allowing all of the perfused fluid to reach the lungs. For reaching the brain, fluids have to cover some distance, in which not all branching vessels (i.e. subclavian arteries) could be clamped. This may have resulted in increased loss of phage. Secondly, the total surface area of the lung vasculature is larger than the total brain surface area.
Identification of the peptide binding site can be an important step in the ligand discovery process. This can give insight into the ligand-target interaction and the uptake mechanism. The binding site should preferably be a receptor, which can specifically internalize a ligand, and the attached drug delivery vehicle, into the cells. Alternatively, nanocarriers such as liposomes can be delivered to transfer their drug load to the target cells without the need for internalization. Lipophilic drugs can be transferred from liposomes to cell membranes when they are brought in close proximity by a targeting ligand (46). This can result in higher uptake of drugs with a low but not absent brain penetration capacity.
The GLA and GYR binding sites and potential uptake mechanisms are as yet unknown.
Because of the negative selection that was performed by perfusion through the heart, the binding site is likely to be brain-endothelium specific. Therefore, it could be a new, yet unidentified receptor, suitable for specific targeting to the blood-brain barrier.
A literature search showed that 4 out of the 34 selected peptides (PFA (47), GLD (48), SAY (49), and HAA (50)) had been selected before by other groups for very diverse purposes. Two possible explanations could account for this observation. These phage may have been selected in diverse settings because they contain biologically relevant motifs, suitable for multiple in vitro and in vivo applications. Alternatively, these motifs may also just enhance phage amplification rates, making them typical background phage that do not offer a specific target interaction.
Our selected peptides, as they have been tested here, are expressed on a phage, displaying five peptides each. Attached to a nanoparticle (e.g. liposome), the number of peptides per particle can be greatly increased. This multivalency may result in a stronger binding of the targeted nanoparticle (i.e. avidity), compared to the phage tested here.
Two new peptides have been identified that may be used for specific targeting to the blood-brain barrier. In the future, these peptides could act as targeting ligands on nanoparticles to enhance uptake of CNS drugs into the brain.
We thank George P. Smith for providing the phage display library and the host bacteria. This work was supported by Top Institute Pharma, project T5-105-1: nanoscience as a tool for improving bioavailability and blood-brain barrier penetration.
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