Pharmaceutical Research

, 26:2630

Reverse Iontophoresis of Amino Acids: Identification and Separation of Stratum Corneum and Subdermal Sources In Vitro

  • Camille C. Bouissou
  • Jean-Philippe Sylvestre
  • Richard H. Guy
  • M. Begoña Delgado-Charro
Research Paper

Abstract

Purpose

To differentiate the stratum corneum (SC) and subdermal sources of amino acids (AAs) extracted by reverse iontophoresis.

Methods

13 zwitterionic AAs were quantified in this in vitro study. Repetitive tape-stripping permitted the distribution of the analytes to be determined in the SC. Iontophoresis experiments were performed in which the subdermal chamber contained either phosphate-buffered saline (PBS) only, or a mixture of the 13 AAs in PBS.

Results

AAs were homogeneously distributed across the SC and broadly divided into three groups (high, medium, low) in terms of total amount present. As expected, extraction to the cathode for the essentially neutral analytes involved was more efficient. Initial samples obtained during the first hour of iontophoresis primarily extracted AAs from the SC. The fluxes observed in the latter half of the 6-h experiment, on the other hand, correlated well with the corresponding subdermal concentrations.

Conclusion

A relatively short extraction period (~1 h) by reverse iontophoresis can be used to evaluate the content of AAs in the SC. Once this ‘reservoir’ has been depleted, reverse iontophoresis can then monitor the subdermal concentrations of the AAs. The latter appears most useful for compounds which are present at lower levels in the SC.

KEY WORDS

amino acids non-invasive monitoring reverse iontophoresis skin reservoir stratum corneum tape-stripping 

ABBREVIATIONS

AA

amino acid

IC-IPAD

ion chromatography with integrated pulsed-amperometric detection

NMF

natural moisturizing factor

SC

stratum corneum

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Camille C. Bouissou
    • 1
  • Jean-Philippe Sylvestre
    • 1
  • Richard H. Guy
    • 1
  • M. Begoña Delgado-Charro
    • 1
  1. 1.Department of Pharmacy & PharmacologyUniversity of BathBathUK

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