Diffusion Studies of Nanometer Polymersomes Across Tissue Engineered Human Oral Mucosa
To measure the diffusion of nanometer polymersomes through tissue engineered human oral mucosa.
In vitro models of full thickness tissue engineered oral mucosa (TEOM) were used to assess the penetration properties of two chemically different polymersomes comprising two of block copolymers, PMPC-PDPA and PEO-PDPA. These copolymers self-assemble into membrane-enclosed vesicular structures. Polymersomes were conjugated with fluorescent rhodamine in order to track polymersome diffusion. Imaging and quantification of the diffusion properties were assessed by confocal laser scanning microscopy (CLSM).
TEOM is morphologically similar to natural oral mucosa. Using CLSM, both formulations were detectable in the TEOM within 6 h and after 48 h both penetrated up to 80 μm into the TEOM. Diffusion of PMPC-PDPA polymersomes was widespread across the epithelium with intra-epithelial uptake, while PEO-PDPA polymersomes also diffused into the epithelium.
CLSM was found to be an effective and versatile method for analysing the level of diffusion of polymersomes into TEOM. The penetration and retention of PMPC-PDPA and PEO-PDPA polymersomes means they may have potential for intra-epithelial drug delivery and/or trans-epithelial delivery of therapeutic agents.
KEY WORDSconfocal laser scanning microscopy diffusion epithelium oral mucosa polymersome
Air liquid interface
Atomic transfer radical polymerisation
Confocal laser scanning microscopy
Poly(ethylene oxide)-poly(2-(diisopropylamino)ethyl methacrylate)
2-(Methacryloyloxy)ethyl phosphorylcholine)-poly(2-(diisopropylamino)ethyl methacrylate
Transmission electron microscopy
Tissue engineered oral mucosa
We would like to thank Dr. Anthony Bullock, Dr. Helen Colley, Mr. Tom Smart and Ms. Marzia Massignani for their help and guidance. This work was supported by funding from EPSRC (DTA PhD studentship to Vanessa Hearnden) and Biocompatibles UK Ltd.
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