Pharmaceutical Research

, Volume 25, Issue 9, pp 2002–2011

Contrast-Enhanced MRI-Guided Photodynamic Cancer Therapy with a Pegylated Bifunctional Polymer Conjugate

  • Anagha Vaidya
  • Yongen Sun
  • Yi Feng
  • Lyska Emerson
  • Eun-Kee Jeong
  • Zheng-Rong Lu
Research Paper

Abstract

Purpose

To study contrast-enhanced MRI guided photodynamic therapy with a pegylated bifunctional polymer conjugate containing an MRI contrast agent and a photosensitizer for minimally invasive image-guided cancer treatment.

Methods

Pegylated and non-pegylated poly-(l-glutamic acid) conjugates containing mesochlorin e6, a photosensitizer, and Gd(III)-DO3A, an MRI contrast agent, were synthesized. The effect of pegylation on the biodistribution and tumor targeting was non-invasively visualized in mice bearing MDA-MB-231 tumor xenografts with MRI. MRI-guided photodynamic therapy was carried out in the tumor bearing mice. Tumor response to photodynamic therapy was evaluated by dynamic contrast enhanced MRI and histological analysis.

Results

The pegylated conjugate had longer blood circulation, lower liver uptake and higher tumor accumulation than the non-pegylated conjugate as shown by MRI. Site-directed laser irradiation of tumors resulted in higher therapeutic efficacy for the pegylated conjugate than the non-pegylated conjugate. Moreover, animals treated with photodynamic therapy showed reduced vascular permeability on DCE-MRI and decreased microvessel density in histological analysis.

Conclusions

Pegylation of the polymer bifunctional conjugates reduced non-specific liver uptake and increased tumor uptake, resulting in significant tumor contrast enhancement and high therapeutic efficacy. The pegylated poly(l-glutamic acid) bifunctional conjugate is promising for contrast enhanced MRI guided photodynamic therapy in cancer treatment.

KEY WORDS

dynamic contrast enhanced MRI image-guided cancer therapy non-invasive pharmacokinetics photodynamic therapy polymer conjugates 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Anagha Vaidya
    • 1
  • Yongen Sun
    • 1
  • Yi Feng
    • 1
  • Lyska Emerson
    • 2
  • Eun-Kee Jeong
    • 3
  • Zheng-Rong Lu
    • 1
    • 4
  1. 1.Department of Pharmaceutics and Pharmaceutical ChemistryUniversity of UtahSalt Lake CityUSA
  2. 2.Department of PathologyUniversity of UtahSalt Lake CityUSA
  3. 3.Department of RadiologyUniversity of UtahSalt Lake CityUSA
  4. 4.421 Wakara WaySalt Lake CityUSA

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