Pharmaceutical Research

, Volume 23, Issue 6, pp 1075–1088

Metabonomics Techniques and Applications to Pharmaceutical Research & Development

  • John C. Lindon
  • Elaine Holmes
  • Jeremy K. Nicholson
Expert Review

Abstract

In this review, the background to the approach known as metabonomics is provided, giving a brief historical perspective and summarizing the analytical and statistical techniques used. Some of the major applications of metabonomics relevant to pharmaceutical Research & Development are then reviewed including the study of various influences on metabolism, such as diet, lifestyle, and other environmental factors. The applications of metabonomics in drug safety studies are explained with special reference to the aims and achievements of the Consortium for Metabonomic Toxicology. Next, the role that metabonomics might have in disease diagnosis and therapy monitoring is provided with some examples, and the concept of pharmacometabonomics as a way of predicting an individual's response to treatment is highlighted. Some discussion is given on the strengths and weaknesses, opportunities of, and threats to metabonomics.

Key Words

biomarkers diagnostics drug safety metabonomics spectroscopy 

Abbreviations

CE

capillary electrophoresis

CLOUDS

classification of unknowns by density superposition

COMET

Consortium for Metabonomic Toxicology

COSY

correlation spectroscopy

CPMG

Carr-Purcell-Meiboom-Gill (an NMR pulse sequence)

CSF

cerebrospinal fluid

DA

discriminant analysis

FT

Fourier transform

GC

gas chromatography

HPLC

high-performance liquid chromatography

IR

infrared

LC

liquid chromatography

LIMS

laboratory information management system

MAS

magic angle spinning

MS

mass spectrometry

NMR

nuclear magnetic resonance

OSC

orthogonal signal correction

PCA

principal component analysis

PLS

partial least squares

QC

quality control

SHY

statistical heterospectroscopy

SMRS

Standard Metabolic Reporting Structures

STOCSY

statistical total correlation spectroscopy

TOCSY

total correlation spectroscopy

TOF

time of flight

TSP

trimethylsilylpropionic acid sodium salt

UPLC

ultra-performance liquid chromatography

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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • John C. Lindon
    • 1
  • Elaine Holmes
    • 1
  • Jeremy K. Nicholson
    • 1
  1. 1.Biological Chemistry, Biomedical Sciences DivisionImperial College LondonLondonUK

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