Pharmaceutical Research

, Volume 22, Issue 12, pp 2163–2173 | Cite as

Cubosome Dispersions as Delivery Systems for Percutaneous Administration of Indomethacin

  • Elisabetta Esposito
  • Rita Cortesi
  • Markus Drechsler
  • Lydia Paccamiccio
  • Paolo Mariani
  • Catia Contado
  • Elisa Stellin
  • Enea Menegatti
  • Francesco Bonina
  • Carmelo Puglia
Research Paper

Purpose

The present study concerns the production and characterization of monooleine (MO) dispersions as drug delivery systems for indomethacin, taken as model anti-inflammatory drug.

Methods

Dispersions were produced by emulsification and homogenization of MO and poloxamer in water. Morphology and dimensional distribution of the disperse phase have been characterized by cryo-transmission electron microscopy and photon correlation spectroscopy, respectively. X-ray diffraction has been performed to determine the structural organization of the disperse phase. Sedimentation field flow fractionation (SdFFF) has been performed to investigate drug distribution in the dispersion. An in vitro diffusion study was conducted by Franz cell associated to stratum corneum epidermis membrane on cubosome dispersions viscosized by carbomer. In vivo studies based on skin reflectance spectrophotometry and tape stripping were performed to better investigate the performance of cubosome as indomethacin delivery system.

Results

Microscopy studies showed the coexistence of vesicles and cubosomes. X-ray diffraction revealed the presence of a bicontinuous cubic phase of spatial symmetry Im3m (Q229). SdFFF demonstrated that no free drug was present in the dispersion. Indomethacin incorporated in viscosized MO dispersions exhibited a lower flux with respect to the analogous formulation containing the free drug in the aqueous phase and to the control formulation based on carbomer gel. Reflectance spectroscopy demonstrated that indomethacin incorporated into MO dispersions can be released in a prolonged fashion. Tape-stripping experiments corroborated this finding.

Conclusions

MO dispersions can be proposed as nanoparticulate systems able to control the percutaneous absorption of indomethacin.

Key Words

cryo-TEM cubosomes reflectance spectroscopy tape stripping X-ray 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Elisabetta Esposito
    • 1
  • Rita Cortesi
    • 1
  • Markus Drechsler
    • 2
  • Lydia Paccamiccio
    • 3
  • Paolo Mariani
    • 3
  • Catia Contado
    • 4
  • Elisa Stellin
    • 1
  • Enea Menegatti
    • 1
  • Francesco Bonina
    • 5
  • Carmelo Puglia
    • 5
  1. 1.Dipartimento di Scienze FarmaceuticheUniversità di FerraraFerraraItaly
  2. 2.Macromolecular Chemistry IIUniversity of BayreuthBayreuthGermany
  3. 3.Dipartimento di Scienze Applicate ai Sistemi Complessi e INFMUniversità Politecnica delle MarcheAnconaItaly
  4. 4.Dipartimento di ChimicaUniversità di FerraraFerraraItaly
  5. 5.Dipartimento di Scienze FarmaceuticheUniversità di CataniaCataniaItaly

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