Pharmaceutical Research

, Volume 22, Issue 9, pp 1471–1479

99mTc-EC-Guanine: Synthesis, Biodistribution, and Tumor Imaging in Animals

  • David J. Yang
  • Kaoru Ozaki
  • Chang-Sok Oh
  • Ali Azhdarinia
  • Thomas Yang
  • Megumi Ito
  • Allison Greenwell
  • Jerry Bryant
  • Saady Kohanim
  • Vincenzo K. Wong
  • E. Edmund Kim
Research Paper

DOI: 10.1007/s11095-005-6157-8

Cite this article as:
Yang, D.J., Ozaki, K., Oh, CS. et al. Pharm Res (2005) 22: 1471. doi:10.1007/s11095-005-6157-8

Purpose

DNA markers are useful in assessing cell proliferation. The purpose of this study was to synthesize 99mTc-ethylenedicysteine-guanine (EC-Guan) for evaluation of cell proliferation.

Methods

Tumor cells were incubated with 99mTc-EC-Guan for cell cycle analysis. Prostate tumor cells that were overexpressing the HSV thymidine kinase gene, or various tumor cells were incubated with 99mTc-EC-Guan at 0.5–2 h. Thymidine incorporation assays were performed in lung cancer cells incubated with EC-Guan at 0.1–1 mg/well. Tissue distribution, autoradiography, and planar scintigraphy of 99mTc-EC-Guan and 99mTc-EC (control) were determined in tumor-bearing rodents at 0.5–4 h.

Results

Cell culture assays indicated that EC-Guan was incorporated in DNA, and there was no significant uptake difference between HSVTK overexpressed and normal groups. Biodistribution and scintigraphic imaging studies of 99mTc-EC-Guan showed increased tumor/tissue count density ratios as a function of time.

Conclusions

Our results indicate that 99mTc-EC-Guan may be useful as a tumor proliferation imaging agent.

Key Words

biodistribution EC-Guan proliferation SPECT 

Abbreviations

EC

ethylenedicysteine

FDG

Fluorodeoxyglucose

FLT

Fluoro-L thymidine

Guan

guanine

PET

positron emission tomography

SPECT

single photon emission computed tomography

Tk

thymidine kinase

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • David J. Yang
    • 1
    • 2
  • Kaoru Ozaki
    • 1
  • Chang-Sok Oh
    • 1
  • Ali Azhdarinia
    • 1
  • Thomas Yang
    • 1
  • Megumi Ito
    • 1
  • Allison Greenwell
    • 1
  • Jerry Bryant
    • 1
  • Saady Kohanim
    • 1
  • Vincenzo K. Wong
    • 1
  • E. Edmund Kim
    • 1
  1. 1.Division of Diagnostic ImagingThe University of Texas MD Anderson Cancer CenterHoustonUSA
  2. 2.Department of Experimental Diagnostic ImagingThe University of Texas MD Anderson Cancer CenterHoustonUSA

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