Pharmaceutical Research

, Volume 22, Issue 4, pp 636–646 | Cite as

A Natural Compound (Ginsenoside Re) Isolated from Panax ginseng as a Novel Angiogenic Agent for Tissue Regeneration

  • Ya-Chun Huang
  • Chiung-Tong Chen
  • Sung-Ching Chen
  • Po-Hong Lai
  • Huang-Chien Liang
  • Yen Chang
  • Lin-Chien Yu
  • Hsing-Wen Sung
Research Papers

No Heading


The primary challenge for tissue engineering is to develop a vascular supply that can support the metabolic needs of the engineered tissues in an extracellular matrix. In this study, the feasibility of using a natural compound, ginsenoside Re, isolated from Panax ginseng in stimulating angiogenesis and for tissue regeneration was evaluated.


Effects of ginsenoside Re on the proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) were examined in vitro. Additionally, angiogenesis and tissue regeneration in a genipin-fixed porous acellular bovine pericardium (extracellular matrix; ECM) incorporated with ginsenoside Re implanted subcutaneously in a rat model were investigated. Basic fibroblast growth factor (bFGF) was used as a control.


It was found that HUVEC proliferation, migration in a Transwell plate, and tube formation on Matrigel were all significantly enhanced in the presence of bFGF or ginsenoside Re. Additionally, effects of ginsenoside Re on HUVEC proliferation, migration, and tube formation were dose-dependent and reached a maximal level at a concentration of about 30 μg/ml. The in vivo results obtained at 1 week postoperatively showed that the density of neocapillaries and the tissue hemoglobin content in the ECMs were significantly enhanced by bFGF or ginsenoside Re. These results indicated that angiogenesis in the ECMs was significantly enhanced by loading with bFGF or ginsenoside Re. At 1 month postoperatively, vascularzied neo-connective-tissue fibrils were found to fill the pores in the ECMs loaded with bFGF or ginsenoside Re.


The aforementioned results indicated that like bFGF, ginsenoside Re-associated induction of angiogenesis enhanced tissue regeneration, supporting the concept of therapeutic angiogenesis in tissue-engineering strategies.

Key words:

acellular tissue angiogenic agent ginsenoside Re tissue regeneration 



basic fibroblast growth factor


extracellular matrix


the ECM loaded with bFGF


the ECM dip-coated in the drug-free gelatin solution


the ECM loaded with ginsenoside Re


hematoxylin and eosin


human umbilical vein endothelial cell




3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide


nitric oxide


nitric oxide synthase


phosphate buffered saline


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Ya-Chun Huang
    • 1
  • Chiung-Tong Chen
    • 2
  • Sung-Ching Chen
    • 1
  • Po-Hong Lai
    • 1
  • Huang-Chien Liang
    • 1
  • Yen Chang
    • 3
  • Lin-Chien Yu
    • 1
  • Hsing-Wen Sung
    • 1
  1. 1.Department of Chemical EngineeringNational Tsing Hua UniversityHsinchuTaiwan, Republic of China
  2. 2.Division of Biotechnology and Pharmaceutical ResearchNational Health Research InstitutesTaipeiTaiwan, Republic of China
  3. 3.Division of Cardiovascular Surgery, Veterans General Hospital-Taichung, and College of MedicineNational Yang-Ming UniversityTaipeiTaiwan, Republic of China

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