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Quantitative Determination of Total Flavonoids in Horse Chestnut Aesculus Hippocastanum Buds

  • V. A. KurkinEmail author
  • P. V. Belov
  • V. M. Ryzhov
MEDICINAL PLANTS
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A quantitative procedure was developed for determining total flavonoids in horse chestnut buds (Aesculus hippocastanum L.) using differential spectrophotometry at 422 nm and recalculation as rhamnocitrin or 7-O-methylkaempferol (3,5,4′-trihydroxy-7-methoxyflavone), the dominant flavonoid in this raw material. The error of a single determination of total flavonoids in horse chestnut buds with 95% confidence probability was ± 2.23%. The contents of total flavonoids in horse chestnut buds varied from (1.24 ± 0.01) to (2.31 ± 0.03)%.

Keywords

horse chestnut Aesculus hippocastanum L. buds flavonoids rhamnocitrin standardization spectrophotometry 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Samara State Medical UniversitySamaraRussia

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