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Pharmaceutical Chemistry Journal

, Volume 52, Issue 12, pp 986–991 | Cite as

Microcolumn HPLC-UV Analysis of Glycyrrhiza Uralensis and Licorice Preparations

  • D. N. OlennikovEmail author
  • I. N. Zilfikarov
  • Cecile Vennos
Article
  • 6 Downloads

A microcolumn HPLC method with UV detection (MC-HPLC-UV) using ProntoSIL-120-5-C18 sorbent (1 × 60 mm, 1 μm) and gradient elution by LiClO4(4.1 M)/HClO4(0.1 M)–MeCN was developed for quantitative analysis of seven compounds (liquiritin-2″-O-glucoside, liquiritin, isoliquiritin-2″-O-apioside, isoliquiritin, licorice-saponin G2, yunganoside E2, glycyrrhizic acid) in Glycyrrhiza uralensis Fisch. roots. Validation of the procedure showed that the metrological parameters were satisfactory. The procedure was used for research on G. uralensis roots growing in Siberia and quantitative analysis of glycyrrhizic acid in 28 commercial licorice preparations.

Keywords

Glycyrrhiza uralensis microcolumn HPLC-UV flavonoids chalcones triterpenes standardization 

Notes

Acknowledgments

The work was sponsored by SB RAS Project No. VI.62.1.8.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • D. N. Olennikov
    • 1
    Email author
  • I. N. Zilfikarov
    • 2
  • Cecile Vennos
    • 3
  1. 1.Institute of General and Experimental Biology Siberian Branch, Russian Academy of SciencesUlan-UdeRussia
  2. 2.Scientific Center of Biomedical Technologies, All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR)MoscowRussia
  3. 3.Regulatory and Medical Scientific AffairsHinwilSwitzerland

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