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Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry

  • P. A. StruchkovEmail author
  • E. S. Mel’nikov
  • V. L. Beloborodov
  • V. K. Kolkhir
  • I. V. Voskoboinikova
MEDICINAL PLANTS
  • 2 Downloads

Angionorm is an original Russian preparation based on the dried aqueous EtOH (25%) extract of a herbal mixture containing horse chestnuts (Aesculus hippocastanum L.), licorice roots (Glycyrrhiza glabra L.), hawthorn fruit (Crataegus), and dog-rose hips (Rosa canina). Areversed-phase HPLC-MS-MS procedure with gradient elution was developed to identify the main constituents in extracts of the mixture and each raw material type. Standards helped to identify 15 compounds of various classes (phenolic and hydroxycinnamic acids and their esters, flavonoids, flavonoid glycosides, triterpene saponins). Peaks of hyperoside, liquiritin, â-glycyrrhizic acid, and escin isomers were identified using MRM transitions, elution order, and literature data. Herbal sources of identified constituents were determined. Marker compounds characteristic of each raw-material type in the mixture extract were proposed.

Keywords

Angionorm HPLC-MS-MS polyphenolic compounds flavonoids saponins 

References

  1. 1.
    S. A. Vichkanova, V. K. Kolkhir, and T. A. Sokol’skaya, Angionorm, An Angioprotective Agent, Medicines from Plants (VILAR Expertise) [in Russian], ADRIS, Moscow (2009), pp. 47 – 54.Google Scholar
  2. 2.
    T. V. Chuiko, V. F. Korsun, and I. V. Voskoboinikova, Med. Alfavit, 1(8), 40 – 44 (2016).Google Scholar
  3. 3.
    P. Montoro, M. Maldini, M. Russo, et al., J. Pharm. Biomed. Anal., 54(3), 535 – 544 (2011).CrossRefPubMedGoogle Scholar
  4. 4.
    P. Liu, B. Yang, and H. Kallio, Food Chem., 121(4), 1188 – 1197 (2010).CrossRefGoogle Scholar
  5. 5.
    E. Hvattum, Rapid Commun. Mass Spectrom., 16, 655 – 662 (2002).CrossRefPubMedGoogle Scholar
  6. 6.
    X. Wu, L. Liu, M. Zhang, et al., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 878(11 – 12), 861 – 867 (2010).CrossRefGoogle Scholar
  7. 7.
    B. Abad-García, S. Garmon-Lobato, L. A. Berrueta, et al., J. Mass Spectrom., 44(7), 1017 – 1025 (2009).CrossRefPubMedGoogle Scholar
  8. 8.
    J. L. Willems, M. M. Khamis, W. Mohammed Saeidet, et al., Anal. Chim. Acta, 933, 164 – 174 (2016).CrossRefPubMedGoogle Scholar
  9. 9.
    M. N. Clifford, K. L. Johnston, S. Knight, et al., J. Agric. Food Chem., 51(10), 2900 – 2911 (2003).CrossRefPubMedGoogle Scholar
  10. 10.
    F. Cuyckens and M. Claeys, Rapid Commun. Mass Spectrom., 16(24), 2341 – 2348 (2002).CrossRefPubMedGoogle Scholar
  11. 11.
    TU 64-4-75-96 Seeds of Aesculus hippocastanum.Google Scholar
  12. 12.
    State Pharmacopoeia of the USSR, Xth Ed., Meditsina, Moscow (1968), p. 573.Google Scholar
  13. 13.
    State Pharmacopoeia of the USSR, XIth Ed., No. 2, Meditsina, Moscow (1987), p. 32.Google Scholar
  14. 14.
    State Pharmacopoeia of the USSR, XIth Ed., No. 2, Meditsina, Moscow (1987), p. 38.Google Scholar
  15. 15.
    T. Cui, K. Nakamura, S. Tian, et al., Biosci. Biotechnol. Biochem., 70(12), 2948 – 2956 (2006).CrossRefPubMedGoogle Scholar
  16. 16.
    W. Huang, M. Wang, H. Shi, et al., Arch. Pharm. Res., 35(11), 1945 – 1952 (2012).CrossRefPubMedGoogle Scholar
  17. 17.
    K. Liu, X. Qiao, Q. Wang, et al., Anal. Methods, 5(19), 5241 – 5247 (2013).CrossRefGoogle Scholar
  18. 18.
    T. Xu, M. Yang, Y. Li, et al., Rapid Commun. Mass Spectrom., 27, 2297 – 2309 (2013).CrossRefPubMedGoogle Scholar
  19. 19.
    G. Li, D. Nikolic and R. B. Van Breemen, J. Agric. Food Chem., 64(42), 8062 – 8070 (2016).CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Y. Hiraga, H. Endo, K. Tanakashi, et al., J. Chromatogr., 292, 451 – 453 (1984).CrossRefGoogle Scholar
  21. 21.
    N. Martins, L. Barros, M. Duenas, et al., RSC Adv., 5, 26991 – 26997 (2015).CrossRefGoogle Scholar
  22. 22.
    K. Tsubone, S. Ohnishi, and T. Yoneya, J. Chromatogr., 248, 469 – 471 (1982).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • P. A. Struchkov
    • 1
    Email author
  • E. S. Mel’nikov
    • 1
  • V. L. Beloborodov
    • 1
  • V. K. Kolkhir
    • 2
  • I. V. Voskoboinikova
    • 2
  1. 1.I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian FederationMoscowRussia
  2. 2.All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural SciencesMoscowRussia

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