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A Novel UV-Spectrophotometric Method for Simultaneous Estimation of Amlodipine and Captopril

  • Ghulam Murtaza
  • Yasmeen Akhtar
  • Tariq Mahmood
  • Syed Mobasher Ali Abid
  • Petre MakreskiEmail author
Article
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An UV-spectrophotometric method for simultaneous determination of amlodipine and captopril has been developed and validated. Simultaneous equation method is applied for the quantification of both drugs, which is based upon the measurement of absorbance at two wavelengths, λmax = 236 and 213 nm for amlodipine and captopril, respectively, in 0.1 N HCl solutions. The calibration curves for amlodipine and captopril are linear in concentration ranges of 0.195 – 100 and 0.39 – 100 μg/mL, respectively. Accuracy of the method is 98 and 99% for amlodipine and captopril, respectively. The inter-day precision for amlodipine and captopril in terms of RSD is 10 and 8%, while the intra-day RSD is 6 and 9%, respectively. Sensitivity of the method in term of the limit of detection for amlodipine and captopril is 0.195 and 0.39 μg, respectively. The method gives 98% reproducible results.

Keywords

amlodipine captopril simultaneous estimation UV-spectrophotometric method validation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ghulam Murtaza
    • 1
  • Yasmeen Akhtar
    • 2
  • Tariq Mahmood
    • 3
  • Syed Mobasher Ali Abid
    • 2
  • Petre Makreski
    • 4
    Email author
  1. 1.Department of PharmacyCOMSATS University IslamabadLahorePakistan
  2. 2.Department of PharmacyCOMSATS University IslamabadAbbottabadPakistan
  3. 3.Department of ChemistryCOMSATS University IslamabadAbbottabadPakistan
  4. 4.Institute of Chemistry, Faculty of Natural Sciences and MathematicsSs. Cyril and Methodius UniversitySkopjeRepublic of Macedonia

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