Development of a Quantitative Determination Procedure for Polyphenolic Compounds in Medicinal Plant Raw Material and Medicinal Herbal Preparations Using TLC with Stepwise Elution

  • O. V. TrineevaEmail author
  • E. F. Safonova
  • A. I. Slivkin

A cost-effective and rapid procedure to identify and quantify polyphenolic biologically active compounds of plant origin (with gallic acid, tannin, and quercetin as examples) using high-performance thin-layer chromatography (HPTLC) was developed. The optimal TLC conditions were chosen experimentally and justified theoretically with quantitative interpretation of the HPTLC data on a PC. The proposed method was tested using medicinal plant raw materials such as nettle leaves, common oak bark, and sea buckthorn fruit. The procedure could be used for quality control of drug substances, single-component and complex preparations, plant materials, biologically active additives, premixes, food items, and cosmetics.


polyphenolic biologically active compounds gallic acid tannin quercetin high-performance TLC 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • O. V. Trineeva
    • 1
    Email author
  • E. F. Safonova
    • 1
  • A. I. Slivkin
    • 1
  1. 1.Voronezh State UniversityMinistry of Education and Science of the RFVoronezhRussia

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