Antiproliferative Evaluation of (E)-3-(3-(Allyloxy)-2-Methoxyphenyl)-1-(2,4,6-Trimethoxyphenyl)Prop-2-En-1-One as a Novel Apoptosis Inducer Against Prostate Cancer PC-3 Cells
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(E)-3-(3-(Allyloxy)-2-methoxyphenyl)-1-(2,4,6-trimethoxyphenyl)prop-2-en-1-one displayed potent antiproliferative activity against the PC-3 cell line. For colony assay, it can inhibit the colony formation at a concentration of 10 μM. Morphological changes of PC-3 cells were determined using DAPI (4′,6-diamidino-2-phenylindole) staining and chromatin condensation. Treatment with this compound at 10 μM induced apoptosis from 2.0% to 30.4% compared with control according to flow cytometry analysis. It also induced the downregulation of apoptosis-related markers Bcl-2 and XIAP (X-linked inhibitor of apoptosis protein). However, treatment with NAC (N-acetyl-L-cysteine) almost completely attenuated chalcone-induced cell inhibition and cell apoptosis in PC-3 cells.
KeywordsPC-3 cells colony formation morphological changes apoptosis Bcl-2 NAC
This work was supported by the National Natural Sciences Foundations of China (Project Nos. 81703541 and 81673322) and project funded by the China Postdoctoral Science Foundation. This work was also supported by MEDCHEMEXPRESS. Thanks to MCE Award for Scientists Promoting Biology and Medicine Research.
- 2.N. N. Yokoyama, S. Shao, B. H. Hoang, et al., Am. J. Clin. Exp. Urol., 2, 27 – 44 (2014).Google Scholar