Hemoglobin as a Diagnosing Molecule for Biological Effects of Atmospheric-Pressure Plasma
- 70 Downloads
The studies with proteins are necessary to understand the biological effects of atmospheric pressure plasma (APP). Among proteins, those with transient metal ions play key roles in many biological events and they are very sensitive to environmental redox states. Iron-containing hemoglobin (Hb) is investigated in this study, after APP treatments under two environmental gas conditions of pure N2 and N2 + O2 mixture. Monitoring the intensity change for absorption spectra could lead to a quantitative assessment of the effect of discharge plasma on Hb. Redox states of Hb are classified into five states including O2-bound Hb (oxy-Hb), deoxy-Hb, met-Hb, NO-bound Hb (NO-Hb), and hemichrome. Chemically generated reactive species and some scavengers are applied to understand the chemical reactions. Our experimental results confirm the complex chemical reactions of APP and suggest the possible use of Hb as a model protein for the visualization of APP biological effects.
KeywordsAtmospheric pressure plasma Hemoglobin Reactive oxygen species (ROS) Reactive nitrogen species (RNS) ROS/RNS scavengers
This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2016K1A4A3914113) and (NRF-2016R1A1A1A05005431). It was partly supported by the research grant of Kwangwoon University in 2016.
Compliance with Ethical Standards
Conflict of interest
The author declares they have no conflicting financial interests.
- 12.Nastuta AV, Topala I, Pohoata V, Mihaila I, Agheorghiesei C, Dumitrascu N (2017) Atmospheric pressure plasma jets in inert gases: electrical, optical and mass spectrometry diagnosis. Rom Rep Phys 69:407Google Scholar
- 13.Takamatsu T, Kawate A, Oshita T, Miyahara H, Okino A, Fridman G (2013) Investigation of reactive species in various gas plasmas treated liquid and sterilization effects. Int Soc Plasma Chem 21:370Google Scholar
- 16.Lukes P, Dolezalova E, Sisrova I, Clupek M (2014) Aqueous-phase chemistry and bactericidal effects from an air discharge plasma in contact with water: evidence for the formation of peroxynitrite through a pseudo-second-order post-discharge reaction of H2O2 and HNO2. Plasma Sources Sci Technol 23:015019CrossRefGoogle Scholar
- 33.Zhang Y, Davies LR, Martin SM, Coddington WJ, Miller FJ, Buettner GR, Kerber RE (2003) The nitric oxide donor S-nitroso-N-acetylpenicillamine (SNAP) increases free radical generation and degrades left ventricular function after myocardial ischemia–reperfusion. Resuscitation 59:345CrossRefPubMedPubMedCentralGoogle Scholar
- 35.Ibrahim MA, El-Gohary MI, Saleh NA, Elashry MY (2008) Spectroscopic study on oxidative reactions of normal and pathogenic hemoglobin molecules. Rom J Biophys 18:39–47Google Scholar
- 40.Itikawa Y, Hayashi M, Ichimura A (1986) Cross sections for collisions of electrons and photons with nitrogen molecules. J Phys Chem 15:985Google Scholar
- 48.Lieberman MA, Lichtenberg AJ (1994) Principles of plasma discharges and materials processing. MRS Bull 30:899–901Google Scholar
- 52.Cano-Europa E, Blas-Valdivia V, Franco-Colin M, Ortiz-Butron R (2015) Regulation of the redox environment. In: Basic principles and clinical significance of oxidative stress. InTech. https://doi.org/10.5772/61515