Plasma Chemistry and Plasma Processing

, Volume 38, Issue 5, pp 937–952 | Cite as

Hemoglobin as a Diagnosing Molecule for Biological Effects of Atmospheric-Pressure Plasma

  • Se Hoon Ki
  • Somin Sin
  • Jae-Ho Shin
  • Young Wan Kwon
  • Myoung Won Chae
  • Han Sup Uhm
  • Ku Youn BaikEmail author
  • Eun Ha ChoiEmail author
Original Paper


The studies with proteins are necessary to understand the biological effects of atmospheric pressure plasma (APP). Among proteins, those with transient metal ions play key roles in many biological events and they are very sensitive to environmental redox states. Iron-containing hemoglobin (Hb) is investigated in this study, after APP treatments under two environmental gas conditions of pure N2 and N2 + O2 mixture. Monitoring the intensity change for absorption spectra could lead to a quantitative assessment of the effect of discharge plasma on Hb. Redox states of Hb are classified into five states including O2-bound Hb (oxy-Hb), deoxy-Hb, met-Hb, NO-bound Hb (NO-Hb), and hemichrome. Chemically generated reactive species and some scavengers are applied to understand the chemical reactions. Our experimental results confirm the complex chemical reactions of APP and suggest the possible use of Hb as a model protein for the visualization of APP biological effects.


Atmospheric pressure plasma Hemoglobin Reactive oxygen species (ROS) Reactive nitrogen species (RNS) ROS/RNS scavengers 



This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2016K1A4A3914113) and (NRF-2016R1A1A1A05005431). It was partly supported by the research grant of Kwangwoon University in 2016.

Compliance with Ethical Standards

Conflict of interest

The author declares they have no conflicting financial interests.

Supplementary material

11090_2018_9917_MOESM1_ESM.pdf (845 kb)
Supplementary material 1 (PDF 844 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Se Hoon Ki
    • 1
  • Somin Sin
    • 2
  • Jae-Ho Shin
    • 2
  • Young Wan Kwon
    • 3
  • Myoung Won Chae
    • 1
  • Han Sup Uhm
    • 1
  • Ku Youn Baik
    • 1
    Email author
  • Eun Ha Choi
    • 1
    Email author
  1. 1.Applied Plasma Medicine CenterKwangwoon UniversitySeoulRepublic of Korea
  2. 2.Department of ChemistryKwangwoon UniversitySeoulRepublic of Korea
  3. 3.KU-KIST Graduate SchoolKorea UniversitySeoulRepublic of Korea

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