Plasma Chemistry and Plasma Processing

, Volume 35, Issue 4, pp 659–676 | Cite as

Effect of Low-Temperature Plasma on the Structure of Seeds, Growth and Metabolism of Endogenous Phytohormones in Pea (Pisum sativum L.)

  • Tibor Stolárik
  • Mária Henselová
  • Michal Martinka
  • Ondřej Novák
  • Anna Zahoranová
  • Mirko Černák
Original Paper


The objective of this study was to determine the influence of low-temperature plasma (LTP) on seed surface modification, water uptake by seeds, seed germination and vigor of seedlings, as well as changes in the content of endogenous hormones in pea, (Pisum sativum L. var. Prophet). The study’s authors used diffuse coplanar surface barrier discharge as the source of LTP in various duration times of treatment (from 60 to 600 s). The SEM analysis showed that LTP induced significant changes on the seeds’ surface, which was related to water permeability into the seeds. LTP increased the germination percentage of pea seeds as well as the growth parameters (root and shoot length, dry weight), and the vigor of seedlings and the effects of LTP also depended on exposure time. The LTP-pretreatment produced changes in endogenous hormones (auxins and cytokinins and their catabolites and conjugates), which correlated with increased growth of the pea seedlings. The results suggested an interaction among the modification of seed structure demonstrated by LTP in the induction of faster germination and hormonal activities related to plant signaling and development during the early growth of pea seedlings.


Endogenous hormones Germination Growth parameters Low-temperature plasma Pea seed structure 



Diffuse coplanar surface barrier discharge


Dry weight


Fresh weight


Low-temperature plasma


Scanning electron microscopy


High voltage



The authors wish to thank the Central Controlling and Testing Institute of Agriculture in Bratislava, Slovakia, for the samples of seeds. The authors are also thankful to Ing. Mária Čaplovičová for the SEM imaging of seeds and Mr. Darren Chastney for critically reviewing the manuscript. This study was supported by the Slovak Grant Agency for Science VEGA No.1/0904/14; by the Slovak Research and Development Agency, Contract No. APVV-14-0264 and by Grant LO1204 from the National Program of Sustainability I, Czech republic.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Tibor Stolárik
    • 1
  • Mária Henselová
    • 2
  • Michal Martinka
    • 2
  • Ondřej Novák
    • 3
  • Anna Zahoranová
    • 4
  • Mirko Černák
    • 4
  1. 1.Department of Biophysics and Centre of the Region, Haná for Agricultural and Biotechnological Research, Faculty of SciencePalacký UniversityOlomoucCzech Republic
  2. 2.Department of Plant Physiology, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovak Republic
  3. 3.Laboratory of Growth Regulators, Faculty of SciencePalacký University and Institute of Experimental Botany AS CROlomoucCzech Republic
  4. 4.Department of Experimental Physics, Faculty of Mathematics, Physics and InformaticsComenius University in BratislavaBratislavaSlovak Republic

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