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Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair

  • Virginia Helena Albarracín
  • Gopal P. Pathak
  • Thierry Douki
  • Jean Cadet
  • Claudio Darío Borsarelli
  • Wolfgang Gärtner
  • María Eugenia Farias
Astrobiology

Abstract

High-Altitude Andean Lakes (HAAL) of the South American Andes are almost unexplored ecosystems of shallow lakes. The HAAL are recognized by a remarkably high UV exposure, strong changes in temperature and salinity, and a high content of toxic elements, especially arsenic. Being exposed to remarkably extreme conditions, they have been classified as model systems for the study of life on other planets. Particularly, Acinetobacter strains isolated from the HAAL were studied for their survival competence under strong UV-B irradiation. Clinical isolates, Acinetobacter baumannii and Acinetobacter johnsonii, served as reference material. Whereas the reference strains rapidly lost viability under UV-B irradiation, most HAAL-derived strains readily survived this exposure and showed less change in cell number after the treatment. Controls for DNA repair activity, comparing dark repair (DR) or photo repair (PR), gave evidence for the involvement of photolyases in the DNA repair. Comparative measurements by HPLC-mass spectrometry detected the number of photoproducts: bipyrimidine dimers under both PR and DR treatments were more efficiently repaired in the HAAL strains (up to 85 % PR and 38 % DR) than in the controls (31 % PR and zero DR ability). Analysis of cosmid-cloned total genomic DNA from the most effective DNA-photorepair strain (Ver3) yielded a gene (HQ443199) encoding a protein with clear photolyase signatures belonging to class I CPD-photolyases. Despite the relatively low sequence similarity of 41 % between the enzymes from Ver3 and from E. coli (PDB 1DNPA), a model-building approach revealed a high structural homology to the CPD-photolyase of E. coli.

Keywords

Acinetobacter Extremophiles High-Altitude Andean Lakes Photolyase UV-resistance 

Notes

Acknowledgments

The authors acknowledge the generous financial support by the PICT-MPI 2006 01090 Project (FONCyT, Argentina) the Proalar Agreement (MINCYT-DAAD) DA/09/05 and MPI Bioinorganic Chemistry, Germany. V.H.A. was supported by DAAD and Marie Curie (FP7-UE) scholarships. M.E.F., C.D.B. and V.H.A. are researchers from the National Research Council (CONICET) in Argentina. The authors gratefully acknowledge helpful discussions with Prof. Dr. Silvia Braslavsky.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Virginia Helena Albarracín
    • 1
    • 2
    • 3
  • Gopal P. Pathak
    • 3
  • Thierry Douki
    • 5
  • Jean Cadet
    • 5
  • Claudio Darío Borsarelli
    • 4
  • Wolfgang Gärtner
    • 3
  • María Eugenia Farias
    • 1
  1. 1.Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CCT, CONICETS. M. de TucumánArgentina
  2. 2.Facultad de Ciencias Naturales e Instituto Miguel LilloUniversidad Nacional de TucumánTucumánArgentina
  3. 3.Max-Planck-Institute for Bioinorganic ChemistryMülheimGermany
  4. 4.Instituto de Química del Noroeste Argentino (INQUINOA), CONICET, Facultad de Agronomía y AgroindustriasUniversidad Nacional de Santiago del EsteroSantiago del EsteroArgentina
  5. 5.Laboratoire “Lésions des Acides Nucléiques” INaC/SCIB UMR-E3 CEA-UJF/CEA-GrenobleGrenoble Cedex 9France

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