Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair

Virginia Helena Albarracín; Gopal P. Pathak; Thierry Douki; Jean Cadet; Claudio Darío Borsarelli; Wolfgang Gärtner; María Eugenia Farias

doi:10.1007/s11084-012-9276-3

Origins of Life and Evolution of Biospheres

June 2012, Volume 42, Issue 2–3, pp 201–221

Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair

Authors
Authors and affiliations

Virginia Helena Albarracín
Gopal P. Pathak
Thierry Douki
Jean Cadet
Claudio Darío Borsarelli
Wolfgang GärtnerEmail author
María Eugenia FariasEmail author

Astrobiology

First Online:: 30 May 2012

Received:: 28 August 2011
Accepted:: 12 December 2011

22 Citations
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Abstract

High-Altitude Andean Lakes (HAAL) of the South American Andes are almost unexplored ecosystems of shallow lakes. The HAAL are recognized by a remarkably high UV exposure, strong changes in temperature and salinity, and a high content of toxic elements, especially arsenic. Being exposed to remarkably extreme conditions, they have been classified as model systems for the study of life on other planets. Particularly, Acinetobacter strains isolated from the HAAL were studied for their survival competence under strong UV-B irradiation. Clinical isolates, Acinetobacter baumannii and Acinetobacter johnsonii, served as reference material. Whereas the reference strains rapidly lost viability under UV-B irradiation, most HAAL-derived strains readily survived this exposure and showed less change in cell number after the treatment. Controls for DNA repair activity, comparing dark repair (DR) or photo repair (PR), gave evidence for the involvement of photolyases in the DNA repair. Comparative measurements by HPLC-mass spectrometry detected the number of photoproducts: bipyrimidine dimers under both PR and DR treatments were more efficiently repaired in the HAAL strains (up to 85 % PR and 38 % DR) than in the controls (31 % PR and zero DR ability). Analysis of cosmid-cloned total genomic DNA from the most effective DNA-photorepair strain (Ver3) yielded a gene (HQ443199) encoding a protein with clear photolyase signatures belonging to class I CPD-photolyases. Despite the relatively low sequence similarity of 41 % between the enzymes from Ver3 and from E. coli (PDB 1DNPA), a model-building approach revealed a high structural homology to the CPD-photolyase of E. coli.

Keywords

Acinetobacter Extremophiles High-Altitude Andean Lakes Photolyase UV-resistance

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Authors and Affiliations

Virginia Helena Albarracín
- 1
- 2
- 3
Gopal P. Pathak
- 3
Thierry Douki
- 5
Jean Cadet
- 5
Claudio Darío Borsarelli
- 4
Wolfgang Gärtner
- 3
Email author
María Eugenia Farias
- 1
Email author

1.Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CCT, CONICETS. M. de TucumánArgentina
2.Facultad de Ciencias Naturales e Instituto Miguel LilloUniversidad Nacional de TucumánTucumánArgentina
3.Max-Planck-Institute for Bioinorganic ChemistryMülheimGermany
4.Instituto de Química del Noroeste Argentino (INQUINOA), CONICET, Facultad de Agronomía y AgroindustriasUniversidad Nacional de Santiago del EsteroSantiago del EsteroArgentina
5.Laboratoire “Lésions des Acides Nucléiques” INaC/SCIB UMR-E3 CEA-UJF/CEA-GrenobleGrenoble Cedex 9France

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Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair

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