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Origins of Life and Evolution of Biospheres

, Volume 37, Issue 2, pp 177–188 | Cite as

Microbial Community of a Hydrothermal Mud Vent Underneath the Deep-Sea Anoxic Brine Lake Urania (Eastern Mediterranean)

  • Michail M. Yakimov
  • Laura Giuliano
  • Simone Cappello
  • Renata Denaro
  • Peter N. Golyshin
Article

Abstract

The composition of a metabolically active prokaryotic community thriving in hydrothermal mud fluids of the deep-sea hypersaline anoxic Western Urania Basin was characterized using rRNA-based phylogenetic analysis of a clone library. The physiologically active prokaryotic assemblage in this extreme environment showed a great genetic diversity. Most members of the microbial community appeared to be affiliated to yet uncultured organisms from similar ecosystems, i.e., deep-sea hypersaline basins and hydrothermal vents. The bacterial clone library was dominated by phylotypes affiliated with the epsilon-Proteobacteria subdivision recognized as an ecologically significant group of bacteria inhabiting deep-sea hydrothermal environments. Almost 18% of all bacterial clones were related to delta-Proteobacteria, suggesting that sulfate reduction is one of the dominant metabolic processes occurring in warm mud fluids. The remaining bacterial phylotypes were related to alpha- and beta-Proteobacteria, Actinobacteria, Bacteroides, Deinococcus-Thermus, KB1 and OP-11 candidate divisions. Moreover, a novel monophyletic clade, deeply branched with unaffiliated 16S rDNA clones was also retrieved from deep-sea sediments and halocline of Urania Basin. Archaeal diversity was much lower and detected phylotypes included organisms affiliated exclusively with the Euryarchaeota. More than 96% of the archaeal clones belonged to the MSBL-1 candidate order recently found in hypersaline anoxic environments, such as endoevaporitic microbial mats, Mediterranean deep-sea mud volcanoes and anoxic basins. Two phylotypes, represented by single clones were related to uncultured groups DHVE-1 and ANME-1. Thus, the hydrothermal mud of hypersaline Urania Basin seems to contain new microbial diversity. The prokaryotic community was significantly different from that occurring in the upper layers of the Urania Basin since 60% of all bacterial and 40% of all archaeal phylotypes were obtained only from mud fluids. The uniqueness of the composition of the active prokaryotic community could be explained by the complex environmental conditions at the site. The interaction of oxygenated warm mud fluids with the cold hypersaline brine of the Urania Basin seems to simultaneously select for various metabolic processes, such as aerobic and anaerobic heterotrophy, sulfide- and methane-dependent chemotrophy along with anaerobic oxidation of methane, sulfate- and metal-reduction.

Keywords

deep-sea hypersaline anoxic basins hydrothermal mud fluids 16S rRNA microbial community structure and function 

Notes

Acknowledgments

This study was supported by the European Commission’s Sustainable Marine Ecosystem program, under BIODEEP (EVK3-2000-00042) and COMMODE (EVK3-2002-00077) Projects. We thank Giuseppe D’Auria for his valuable help at sea and in the laboratory. We thank the crew and the pilot of RV Urania for their expert handling of our samplers and equipment at the Urania Basin and for highly productive cruises. P.N.G. acknowledges the support of DFG, Project Nr Ti86/8. Priority Program ‘Mars and Terrestrial Planets’, and GenoMikPlus Program of the Federal Ministery of Science and Education (BMBF).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Michail M. Yakimov
    • 1
  • Laura Giuliano
    • 1
  • Simone Cappello
    • 1
  • Renata Denaro
    • 1
  • Peter N. Golyshin
    • 2
  1. 1.Istituto per l’Ambiente Marino CostieroCNRMessinaItaly
  2. 2.Technical University BraunschweigBraunschweigGermany

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