Measurement of human serum albumin concentration using Raman spectroscopy setup
- 247 Downloads
This work is dedicated to the measurement of plasma proteins concentration in liquid solutions using Raman spectroscopy setup. The obtained Raman spectra showed significant variation of intensities of certain spectral bands 940, 1005, 1330, 1450 and 1650 cm−1 for different protein fractions and concentrations. Partial least squares regression analysis was used for determination of correlation coefficients. It has been shown that the proposed method represents structure and biochemical composition of albumin and immunoglobulins A and G, and that albumin concentration may be measured with an accuracy of 6–8 g/L.
KeywordsRaman spectroscopy PLS regression Blood proteins Albumin Immunoglobulin
This research was supported by the Ministry of Education and Science of the Russian Federation.
- Gonzàlez-Quintela, A., Alende, M.R., Gamallo, R., Gonzàlez-Gil, P., López-Ben, S., Tomé, S., Otero, E., Torre, J.A.: Serum immunoglobulins (IgG, IgA, IgM) in chronic hepatitis C. A comparison with non-cirrhotic alcoholic liver disease. Hepatogastroenterology 50(54), 2121–2126 (2003)Google Scholar
- Rhoades, R.A., Pflanzer, R.G.: Human Physiology, 4th edn. Thomson Learning, Stockholm (2002)Google Scholar
- Wang, J., Lin, D., Lin, J., Yu, Y., Huang, Z., Chen, Y., Lin, J., Feng, S., Li, B., Liu, N., Chen, R.: Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening. J. Biomed. Opt. 19(8), 087003 (2014). doi: 10.1117/1.JBO.19.8.087003 ADSCrossRefGoogle Scholar
- Zakharov, V.P., Bratchenko, I.A., Artemyev, D.N., Myakinin, O.O., Kornilin, D.V., Kozlov, S.V., Moryatov, A.A.: Comparative analysis of combined spectral and optical tomography methods for detection of skin and lung cancers. J. Biomed. Opt. 20(2), 025003 (2015). doi: 10.1117/1.JBO.20.2.025003. ADSCrossRefGoogle Scholar