The calcium binding proteins calbindin, parvalbumin, and calretinin have specific patterns of expression in the gray matter of cat spinal cord
- 225 Downloads
Calcium binding proteins (CBPs) regulate intracellular levels of calcium (Ca2+) ions. CBPs are particularly interesting from a morphological standpoint, because they are differentially expressed in certain sub-populations of cells in the nervous system of various species of vertebrate animals. However, knowledge on the cellular regulation governing such cell-specific CBP expression is still incomplete. In this work on the L7 segment of the cat spinal cord, we analyzed the localization and morphology of neurons expressing the CBPs calbindin-28 KD (CB), parvalbumin (PV), and calretinin (CR), and co-expressing CB and PV, CB and CR, and PV and CR. Single CBP-positive (+) neurons showed specific distributions: (1) CB was present in small neurons localized in laminae I, II, III and X, in small to medium size neurons in laminae III–VI, and in medium to large neurons in laminae VI–VIII; (2) PV was present in small size neurons in laminae III and IV and in medial portions of laminae V and VI, medium neurons and in lamina X at the border with lamina VII, in medium to large neurons in laminae VII and VIII; (3) CR labeling was detected in small size neurons in laminae I, II, III and VIII, in medium to large size neurons in laminae I and III–VII, and in small to medium size neurons in lamina X. Double labeled neurons were a small minority of the CBP+ cells. Co-expression of CB and PV was seen in 1 to 2% of the CBP+ cells, and they were detected in the ventral and intermediate portions of lamina VII and in lamina X. Co-localization of CB and CR was present in 0.3% of the cells and these cells were localized in lamina II. Double labeling for PV and CR occurred in 6% of the cells, and the cells were localized in ventral part of lamina VII and in lamina VIII. Overall, these results revealed distinct and reproducible patterns of localization of the neurons expressing single CBPs and co-expressing two of them. Distinct differences of CBP expression between cat and other species are discussed. Possible relations between the cat L7 neurons expressing different CBPs with the neurons previously analyzed in cat and other animals are suggested.
Unable to display preview. Download preview PDF.
- ANTAL, M., POLGAR, E., CHALMERS, J., MINSON, J. B., LLEWELLYN-SMITH, I., HEIZMANN, C. W. & SOMOGYI, P. (1991) Different populations of parvalbumin- and calbindin-D28k-immunoreactive neurons contain GABA and accumulate 3H-D-aspartate in the dorsal horn of the rat spinal cord. Journal of Comparative Neurology 314, 114–124.PubMedCrossRefGoogle Scholar
- BARAKAT-WALTER, I., KRAFTSIK, R., KUNTZER, T., BOGOUSSLAVSKY, J. & MAGISTRETTI, P. (2000) Differential effect of thyroid hormone deficiency on the growth of calretinin-expressing neurons in rat spinal cord and dorsal root ganglia. Journal of Comparative Neurology 426, 519–533.PubMedCrossRefGoogle Scholar
- ENEVOLDSON, T. P. & GORDON, G. (1989) Postsynaptic dorsal column neurons in the cat: A study with retrograde transport of horseradish peroxidase. Experimental Brain Research 75, 611–620.Google Scholar
- LEE, R. H. & HECKMAN, C. J. (1998a) Bistability in spinal motoneurons in vivo: Systematic variations in persistent inward currents. Journal of Neurophysiology 80, 583–593.Google Scholar
- LEE, R. H. & HECKMAN, C. J. (1998b) Bistability in spinal motoneurons in vivo: systematic variations in rhythmic firing patterns. Journal of Neurophysiology 80, 572–582.Google Scholar
- RABOISSON, P., DALLEL, R., BERNARD, J. F., LE BARS, D. & VILLANUEVA, L. (1996) Organization of efferent projections from the spinal cervical enlargement to the medullary subnucleus reticularis dorsalis and the adjacent cuneate nucleus: a PHA-L study in the rat. Journal of Comparative Neurology 367, 503–517.PubMedCrossRefGoogle Scholar
- ROGERS, J., KHAN, M. & ELLIS, J. (1990) Calretinin and other CaBPs in the nervous system. Advances Experimental in Medical Biology 269, 195–203.Google Scholar
- TOLEDO-RODRIGUEZ, M., BLUMENFELD, B., WU, C., LUO, J., ATTALI, B., GOODMAN, P. & MARKRAM, H. (2004) Correlation Maps Allow Neuronal Electrical Properties to be Predicted from Single-cell Gene Expression Profiles in Rat Neocortex. Cerebral Cortex. (Advance Access published) June 10.Google Scholar
- WILLIS, W. D. (1995) Pain. In The Rat Nervous System (edited by ACADEMIC PRESS), pp. 725–750. Sydney.Google Scholar