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Journal of Neurocytology

, Volume 34, Issue 1–2, pp 135–147 | Cite as

The vomeronasal organ of greater bushbabies (Otolemur spp.): Species, sex, and age differences

  • Timothy D. SmithEmail author
  • Kunwar P. Bhatnagar
  • Anne M. Burrows
  • Kristin L. Shimp
  • John C. Dennis
  • Matthew A. Smith
  • Lisette Maico-Tan
  • Edward E. Morrison
Article

Abstract

The present study examined interspecies, intersexual, and age-related changes in size of the vomeronasal neuroepithelium (VNNE) of two species of greater bushbabies (genus Otolemur, Infraorder Lorisiformes, Suborder Strepsirrhini). Tissue blocks containing the vomeronasal organs of nine O. crassicaudatus (8 adults, 1 neonate) and ten O. garnettii (9 adults, 1 neonate) were studied by means of serial paraffin sectioning and computer-based reconstruction of VNNE volume. In addition, the immunoreactivity of the VNNE to two neuronal markers, neuron-specific beta tubulin (BT) and olfactory marker protein (OMP) was compared between species, sexes, and ages. Results indicated that a clear VNNE is present at birth in both species, and OMP immunoreactivity was verified in O. garnettii at birth. Male and female adults of both species showed OMP-immunoreactive and BT-immunoreactive neurons in the VNNE. Immunohistochemical findings indicated that all males and the youngest females had the thickest VNNE, especially at the marginal junctions with the receptor-free epithelium. Results of a 2-way Analysis of Variance (ANOVA, species x sex) revealed no significant differences in VNNE length or volume between species, but O. crassicaudatus had significantly (p < 0.05) greater palatal length. Significant (p < 0.05) differences also were found between sexes in VNNE volume, but no significant differences in palatal length or VNNE length. The distribution of VNNE volume against age indicated that the sex differences were more pronounced in O. crassicaudatus than O. garnettii. For both species and sexes, distribution of VNNE volume against age suggested an age-related reduction in volume. These findings demonstrate postnatal plasticity in VNNE size in Otolemur that is reminiscent of that found for olfactory structures in some rodents. Bushbabies or other strepsirrhine primates may offer an opportunity for further understanding of behavioral correlates of VNNE postnatal plasticity, which may represent primitive functional characteristics of the order Primates.

Keywords

Young Female Neuronal Marker Female Adult Immunohistochemical Finding Behavioral Correlate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BT

beta tubulin

IHC

immunohistochemistry

OMP

olfactory marker protein

RFE

receptor-free epithelium

VNNE

vomeronasal neuroepithelium

VNO

vomeronasal organ

VRN

vomeronasal receptor neurons

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Timothy D. Smith
    • 1
    • 2
    Email author
  • Kunwar P. Bhatnagar
    • 3
  • Anne M. Burrows
    • 2
    • 4
  • Kristin L. Shimp
    • 1
  • John C. Dennis
    • 5
  • Matthew A. Smith
    • 6
  • Lisette Maico-Tan
    • 1
  • Edward E. Morrison
    • 5
  1. 1.School of Physical TherapySlippery Rock UniversitySlippery Rock
  2. 2.Department of AnthropologyUniversity of PittsburghPittsburgh
  3. 3.Department of Anatomical Sciences and NeurobiologyUniversity of Louisville School of MedicineLouisville
  4. 4.Department of Physical TherapyDuquesne UniversityPittsburgh
  5. 5.Department of Anatomy, Physiology and Pharmacology, College of Veterinary MedicineAuburn UniversityAuburn
  6. 6.Center for the Neural Basis of CognitionCarnegie Mellon UniversityPittsburgh

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