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Journal of Neurocytology

, Volume 33, Issue 6, pp 607–615 | Cite as

Lingual deficits in neurotrophin double knockout mice

  • Irina V. Nosrat
  • Karin Agerman
  • Andrea Marinescu
  • Patrik Ernfors
  • Christopher A. NosratEmail author
Article

Abstract

Brain-derived neurotrophic factor (BDNF) and Neurotrophin 3 (NT-3) are members of the neurotrophin family and are expressed in the developing and adult tongue papillae. BDNF null-mutated mice exhibit specific impairments related to innervation and development of the gustatory system while NT-3 null mice have deficits in their lingual somatosensory innervation. To further evaluate the functional specificity of these neurotrophins in the peripheral gustatory system, we generated double BDNF/NT-3 knockout mice and compared the phenotype to BDNF−/− and wild-type mice. Taste papillae morphology was severely distorted in BDNF−/−xNT-3−/− mice compared to single BDNF−/− and wild-type mice. The deficits were found throughout the tongue and all gustatory papillae. There was a significant loss of fungiform papillae and the papillae were smaller in size compared to BDNF−/− and wild-type mice. Circumvallate papillae in the double knockouts were smaller and did not contain any intraepithelial nerve fibers. BDNF−/−xNT-3−/− mice exhibited additive losses in both somatosensory and gustatory innervation indicating that BDNF and NT-3 exert specific roles in the innervation of the tongue. However, the additional loss of fungiform papillae and taste buds in BDNF−/−xNT-3−/− mice compared to single BDNF knockout mice indicate a synergistic functional role for both BDNF-dependent gustatory and NT-3-dependent somatosensory innervations in taste bud and taste papillae innervation and development.

Keywords

Knockout Mouse Double Knockout Double Knockout Mouse Fungiform Papilla Gustatory System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Irina V. Nosrat
    • 1
  • Karin Agerman
    • 2
  • Andrea Marinescu
    • 1
  • Patrik Ernfors
    • 2
  • Christopher A. Nosrat
    • 1
    Email author
  1. 1.Laboratory of Oral Neurobiology, Department of Biological and Materials Sciences, School of DentistryUniversity of MichiganAnn Arbor
  2. 2.Unit of Molecular Neurobiology, MBBKarolinska InstitutetStockholmSweden

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