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Neurochemical Research

, Volume 44, Issue 12, pp 2776–2785 | Cite as

Dysregulated Transcription Factor TFAP2A After Peripheral Nerve Injury Modulated Schwann Cell Phenotype

  • Fuchao Zhang
  • Xiaokun Gu
  • Sheng YiEmail author
  • Hui XuEmail author
Original Paper
  • 76 Downloads

Abstract

Transcription factors regulate the transcriptions and expressions of numerous target genes and direct a variety of physiological and pathological activities. To obtain a better understanding of the involvement of transcription factors during peripheral nerve repair and regeneration, significantly differentially expressed genes coding for transcription factors in rat sciatic nerves after sciatic nerve crush injury were identified. A total of 9 transcription factor genes, including GBX2, HIF3A, IRF8, LRRC63, SNAI3, SPIB, TBX21, TFAP2A, and ZBTB16 were identified to be commonly differentially expressed at 1, 4, 7, and 14 days after nerve injury. TFAP2A, a gene encoding transcription factor activating enhancer binding protein 2 alpha, was found to be critical in the regulatory network. PCR validation and immunohistochemistry staining of injured rat sciatic nerves showed that TFAP2A expression was significantly up-regulated in the Schwann cells after nerve injury for at least 2 weeks. Schwann cells transfected with TFAP2A-siRNA exhibited elevated proliferation rate and migration ability, suggesting that TFAP2A suppressed Schwann cell proliferation and migration. Collectively, our study provided a global overview of the dynamic changes of transcription factors after sciatic nerve injury, discovered key transcription factors for the regeneration process, and deepened the understanding of the molecular mechanisms underlying peripheral nerve repair and regeneration.

Keywords

Transcription factor TFAP2A Peripheral nerve injury Schwann cells Proliferation Migration 

Notes

Author Contributions

Conceived and designed the experiments: SY HX. Performed the experiments: FZ XG. Analyzed the data: FZ XG SY. Contributed reagents/materials/analysis tools: SY. Wrote the manuscript: SY HX.

Funding

This work was supported by the National Natural Science Foundation of China [31700926], and the Priority Academic Program Development of Jiangsu Higher Education Institutions of China [PAPD].

Compliance with Ethical Standards

Ethics Approval and Consent to Participate

All the experimental procedures involving animals were conducted in accordance with Institutional Animal Care guidelines of Nantong University and approved ethically by the Administration Committee of Experimental Animals, Jiangsu, China.

Supplementary material

11064_2019_2898_MOESM1_ESM.xlsx (13 kb)
Table S1List of significantly differentially expressed transcription factors in the sciatic nerves at 1, 4, 7, and 14 days after crush injury. Gene Symbol, relative expression level (Log Ratio), FDR (q-value), gene ID, gene name, and gene localization were labeled. Supplementary material 1 (XLSX 13.0 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of NeuroregenerationNantong UniversityNantongChina
  2. 2.Department of Hand SurgeryAffiliated Hospital of Nantong UniversityNantongChina

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