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Effects of Restraint Water-Immersion Stress-Induced Gastric Mucosal Damage on Astrocytes and Neurons in the Nucleus Raphe Magnus of Rats via the ERK1/2 Signaling Pathway

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Abstract

Restraint water-immersion stress (RWIS) consists of psychological and physical stimulation, and it has been utilized in the research of gastric mucosal damage. It has been shown by previous studies that the nucleus raphe magnus (NRM) is closely involved in the gastrointestinal function, but its functions on the stress-induced gastric mucosal injury (SGMI) have not been thoroughly elucidated to date. Consequently, in this research, we aim to measure the expression of astrocytic glial fibrillary acidic protein (GFAP), neuronal c-Fos, and phosphorylation extracellular signal regulated kinase 1/2 (p-ERK1/2) in the process of RWIS with immunohistochemistry and western blot methods. What is more, we detect the relation between astrocytes and neurons throughout the stress procedure and explore the regulation of the ERK1/2 signaling pathway on the activity of astrocytes and neurons after RWIS. The results indicated that all three proteins expression multiplied following peaked 3 h substantially. The SMGI, astrocyte and neuron activity were affected after the astrocytotoxin L-A-aminohexanedioic acid (L-AA) and c-fos antisense oligonucleotide (ASO) injections. After the injection of PD98059, the gastric mucosal injury, astrocyte and neuron activity significantly fell off. These results suggested that RWIS-induced activity of astrocytes and neurons in the NRM may play a significant part in gastric mucosa damage via the ERK1/2 signaling pathway.

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Funding

This work was supported according to the National Natural Science Foundation of China (No. 31672286) and the Natural Science Foundation of Shandong Province, China (ZR2013CM010, ZR2012HM061, and ZR2018BC024).

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Authors and Affiliations

  1. Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, 250014, China

    Fangcheng Fan & Haiji Sun

  2. Qingdao No. 31 Middle School, Qingdao, 266041, China

    Mengzhu Yang

  3. Shandong Traditional Chinese Medicine University, Jinan, 250014, China

    Xiwen Geng

  4. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China

    Xiaoli Ma

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  1. Fangcheng Fan
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  2. Mengzhu Yang
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  3. Xiwen Geng
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  4. Xiaoli Ma
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  5. Haiji Sun
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Contributions

XM and HS formulated the study’s design. FC, MY, and XG carried the learning out and analyzed the experimental data. The manuscript was drafted by FC. XM and HS corrected the paper for significant noetic content.

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Correspondence to Xiaoli Ma or Haiji Sun.

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Fan, F., Yang, M., Geng, X. et al. Effects of Restraint Water-Immersion Stress-Induced Gastric Mucosal Damage on Astrocytes and Neurons in the Nucleus Raphe Magnus of Rats via the ERK1/2 Signaling Pathway. Neurochem Res 44, 1841–1850 (2019). https://doi.org/10.1007/s11064-019-02818-0

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  • DOI: https://doi.org/10.1007/s11064-019-02818-0

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