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SSeCKS promoted lipopolysaccharide-sensitized astrocytes migration via increasing β-1,4-galactosyltransferase-I activity

  • Hua Wei
  • Leiting Xu
  • Chunmiao Li
  • Lianliang Liu
  • Derry Minyao Ng
  • Maria Haleem
  • Lingli Jiang
  • Ning Sun
  • Qingzhi Ling
  • Shaohua Ma
  • Lingli Zhang
  • Qinwen WangEmail author
  • Tao TaoEmail author
Original Paper
  • 13 Downloads

Abstract

Astrocytes migration is essential in the formation of the glial scar during the injury response process of the central nervous system (CNS) especially during inflammation. Integrin β1 is part of the extracellular matrix receptors in the CNS and it has been reported that integrin β-deficient astrocytes randomly migrate into wounds. Previous studies have found that β-1,4 Galactosyltransferase-I (β-1,4-GalT-I) enhanced the β-1,4-galactosylation of integrin β1. Src-suppressed C kinase substrate (SSeCKS) is an inflammatory response protein which functionally interacts with β-1,4 Galactosyltransferase-I (β-1,4-GalT-I). In this study we aim to investigate the role of SSeCKS and β-1,4-GalT-I in the migration of astrocytes during lipopolysaccharide (LPS)-induced inflammation. Coimmunoprecipitation and immunofluorescence assays have demonstrated that SSeCKS and β-1,4-GalT-I were significantly enhanced in LPS-treated astrocytes and their interactions may occur in the Trans-Golgi Network. Lectin blot showed that the knockdown of β-1,4-GalT-I could inhibit the β-1,4-galactosylation of glycoproteins including integrin β1 with and without LPS, and that SSeCKS knockdown inhibits the β-1,4-galactosylation of glycoproteins including integrin β1 only in LPS-induced astrocytes. Additionally, wound healing assays indicated that β-1,4-GalT-I knockdown could inhibit astrocytes migration with and without LPS but SSeCKS inhibited cell migration only when LPS was present. Therefore our findings suggest that SSeCKS affects astrocytes migration by regulating the β-1,4-galactosylation of glycoproteins including integrin β1, via β-1,4-GalT-I expression in LPS-sensitized astrocytes.

Keywords

Astrocytes Migration SSeCKS β-1,4-Galactosyltransferase-I Lipopolysaccharide 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31600402), China Postdoctoral Science Fund (Grant No. 2017M621895), Zhejiang Province Postdoctoral Research Fund (Grant No. ZX2016000849), Ningbo Postdoctoral Research Fund (Grant No. ZX2017000053), Natural Science Foundation of Ningbo (Grant No. 2017A610216, 2016A610205), Natural Science Foundation of Zhejiang Province (Grant No. Y16H070001), Research Project of Zhejiang Provincial Department of Education (Y201738586), Agricultural Project of Public Welfare Technology Research in Zhejiang Provincial Science and Technology Department (Grant No. ZX2014C32047).

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hua Wei
    • 1
    • 2
  • Leiting Xu
    • 2
  • Chunmiao Li
    • 3
  • Lianliang Liu
    • 2
  • Derry Minyao Ng
    • 2
  • Maria Haleem
    • 2
  • Lingli Jiang
    • 1
  • Ning Sun
    • 1
  • Qingzhi Ling
    • 1
  • Shaohua Ma
    • 1
  • Lingli Zhang
    • 1
  • Qinwen Wang
    • 2
    Email author
  • Tao Tao
    • 1
    • 2
    Email author
  1. 1.Ningbo College of Health SciencesNingboPeople’s Republic of China
  2. 2.Ningbo UniversityNingboPeople’s Republic of China
  3. 3.Medical School of Nantong UniversityNantongPeople’s Republic of China

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