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Effect of Silk Fibroin on Neuroregeneration After Traumatic Brain Injury

  • M. M. Moisenovich
  • E. Y. Plotnikov
  • A. M. Moysenovich
  • D. N. Silachev
  • T. I. Danilina
  • E. S. Savchenko
  • M. M. Bobrova
  • L. A. Safonova
  • V. V. Tatarskiy
  • M. S. Kotliarova
  • I. I. Agapov
  • D. B. Zorov
Original Paper
  • 27 Downloads

Abstract

Traumatic brain injury is one of the leading causes of disability among the working-age population worldwide. Despite attempts to develop neuroprotective therapeutic approaches, including pharmacological or cellular technologies, significant advances in brain regeneration have not yet been achieved. Development of silk fibroin-based biomaterials represents a new frontier in neuroregenerative therapies after brain injury. In this study, we estimated the short and long-term effects of silk fibroin scaffold transplantation on traumatic brain injury and biocompatibility of this biomaterial within rat neuro-vascular cells. Silk fibroin microparticles were injected into a brain damage area 1 day after the injury. Silk fibroin affords neuroprotection as judged by diminished brain damage and recovery of long-term neurological functions. We did not detect considerable toxicity to neuro-vascular cells cultured on fibroin/fibroin-gelatin microparticles in vitro. Cultivation of primary cell cultures of neurons and astrocytes on silk fibroin matrices demonstrated their higher viability under oxygen-glucose deprivation compared to 2D conditions on plastic plates. Thus, we conclude that scaffolds based on silk fibroin can become the basis for the creation of constructs aimed to treat brain regeneration after injury.

Keywords

Scaffold Neurons Astrocytes Neuroprotection Fibroin Ischemia 

Notes

Acknowledgements

The reported study was funded by RFBR according to the research project no. 17-00-00359 and Russian Science Foundation (14-24-00107—model of the brain trauma used for animals, and OGD model used for cells).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • M. M. Moisenovich
    • 1
  • E. Y. Plotnikov
    • 2
  • A. M. Moysenovich
    • 1
  • D. N. Silachev
    • 2
  • T. I. Danilina
    • 3
  • E. S. Savchenko
    • 3
  • M. M. Bobrova
    • 1
    • 4
  • L. A. Safonova
    • 1
    • 4
  • V. V. Tatarskiy
    • 5
  • M. S. Kotliarova
    • 1
  • I. I. Agapov
    • 1
    • 4
  • D. B. Zorov
    • 2
  1. 1.Biological FacultyLomonosov Moscow State UniversityMoscowRussia
  2. 2.A.N. Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia
  4. 4.Bionanotechnology LaboratoryV.I.Shumakov National Medical Research Center of Transplantology and Artificial OrgansMoscowRussia
  5. 5.N.N. Blokhin Russian Cancer Research CenterMoscowRussia

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