Neurochemical Research

, Volume 44, Issue 6, pp 1375–1386 | Cite as

Platelets Bioenergetics Screening Reflects the Impact of Brain Aβ Plaque Accumulation in a Rat Model of Alzheimer

  • Federico A. Prestia
  • Pablo Galeano
  • Pamela V. Martino Adami
  • Sonia Do Carmo
  • Eduardo M. Castaño
  • A. Claudio Cuello
  • Laura MorelliEmail author
Original Paper


Alzheimer’s disease (AD) is associated to depressed brain energy supply and impaired cortical and hippocampal synaptic function. It was previously reported in McGill-R-Thy1-APP transgenic (Tg(+/+)) rats that Aβ deposition per se is sufficient to cause abnormalities in glucose metabolism and neuronal connectivity. These data support the utility of this animal model as a platform for the search of novel AD biomarkers based on bioenergetic status. Recently, it has been proposed that energy dysfunction can be dynamically tested in platelets (PLTs) of nonhuman primates. PLTs are good candidates to find peripheral biomarkers for AD because they may reflect in periphery the bioenergetics deficits and the inflammatory and oxidative stress processes taking place in AD brain. In the present study, we carried out a PLTs bioenergetics screening in advanced-age (12–14 months old) control (WT) and Tg(+/+) rats. Results indicated that thrombin-activated PLTs of Tg(+/+) rats showed a significantly lower respiratory rate, as compared to that measured in WT animals, when challenged with the same dose of FCCP (an uncoupler of oxidative phosphorylation). In summary, our results provide original evidence that PLTs bioenergetic profiling may reflect brain bioenergetics dysfunction mediated by Aβ plaque accumulation. Further studies on human PLTs from control and AD patients are required to validate the usefulness of PLTs bioenergetics as a novel blood-based biomarker for AD.


Alzheimer’s disease Brain dysfunction Platelet bioenergetic Transgenic rat Amyloid β Blood-based bioenergetic profiling 



We acknowledge the helpful assistance of the personnel of the Laboratory of Hospital Naval Cirujano Dr. Pedro Mallo (Ciudad de Buenos Aires-Argentina) on the determination of the haematologic indices in animals. We wish to thank Dr. Mirta Schattner and Dr. Roberto G. Pozner of the Laboratory of Experimental Thrombosis- Academia Nacional de Medicina (Ciudad de Buenos Aires-Argentina) for their support on the evaluation of platelet aggregation.


This study was supported by funding from the Agencia Nacional de Promoción Científica y Tecnológica (Grant Nos. PICT-2015-0285, PICT-2016-4647 and PIBT/09 2013 to LM; PICT-2013-318 to EMC), Consejo Nacional de Investigaciones Científicas y Técnicas (Grant No. PIP-0378 to LM), Canadian Institutes of Health Research (Grant Nos. 201603PJT-364544 to ACC). FAP is supported by FONCyT fellowship. PVMA is supported by CONICET fellowship. PG, EMC, and LM are members of the Research Career of CONICET. SDC is the holder of the Charles E. Frosst/Merck Research Associate position. ACC is member of the Canadian Consortium of Neurodegeneration in Aging (CCNA) and holder of the McGill University Charles E. Frosst/Merck Chair in Pharmacology.

Supplementary material

11064_2018_2657_MOESM1_ESM.pdf (210 kb)
Supplementary material 1 (PDF 210 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Amyloidosis and NeurodegenerationFundación Instituto Leloir, IIBBA-CONICETCiudad Autónoma de Buenos AiresArgentina
  2. 2.Department of Pharmacology and TherapeuticsMcGill UniversityMontrealCanada

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