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Neurochemical Research

, Volume 43, Issue 12, pp 2268–2276 | Cite as

Intracardiac Injection of Dental Pulp Stem Cells After Neonatal Hypoxia-Ischemia Prevents Cognitive Deficits in Rats

  • Eduardo Farias SanchesEmail author
  • Lauren Valentim
  • Felipe de Almeida Sassi
  • Lisiane Bernardi
  • Nice Arteni
  • Simone Nardin Weis
  • Felipe Kawa Odorcyk
  • Patricia Pranke
  • Carlos Alexandre Netto
Original Paper
  • 160 Downloads

Abstract

Neonatal hypoxia-ischemia (HI) is associated to cognitive and motor impairments and until the moment there is no proven treatment. The underlying neuroprotective mechanisms of stem cells are partially understood and include decrease in excitotoxicity, apoptosis and inflammation suppression. This study was conducted in order to test the effects of intracardiac transplantation of human dental pulp stem cells (hDPSCs) for treating HI damage. Seven-day-old Wistar rats were divided into four groups: sham-saline, sham-hDPSCs, HI-saline, and HI-hDPSCs. Motor and cognitive tasks were performed from postnatal day 30. HI-induced cognitive deficits in the novel-object recognition test and in spatial reference memory impairment which were prevented by hDPSCs. No motor impairments were observed in HI animals. Immunofluorescence analysis showed human-positive nuclei in hDPSC-treated animals closely associated with anti-GFAP staining in the lesion scar tissue, suggesting that these cells were able to migrate to the injury site and could be providing support to CNS cells. Our study evidence novel evidence that hDPSC can contribute to the recovery following hypoxia-ischemia and highlight the need of further investigation in order to better understand the exact mechanisms underlying its neuroprotective effects.

Keywords

Dental pulp stem cells hDPSCs Neonatal hypoxia-ischemia Cellular therapy Memory 

Notes

Acknowledgements

We thank The National Council for Scientific and Technological Development (CNPq), FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul) and Stem Cell Research Institute (SCRI) for their financial support.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Eduardo Farias Sanches
    • 2
    Email author
  • Lauren Valentim
    • 1
    • 2
  • Felipe de Almeida Sassi
    • 1
    • 2
  • Lisiane Bernardi
    • 1
  • Nice Arteni
    • 2
  • Simone Nardin Weis
    • 2
  • Felipe Kawa Odorcyk
    • 2
  • Patricia Pranke
    • 1
    • 3
  • Carlos Alexandre Netto
    • 2
  1. 1.Haematology and Stem Cell Laboratory, Faculty of PharmacyUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Brain Ischemia and Neuroprotection Laboratory, Departament of BiochemistryUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Stem Cell Research InstitutePorto AlegreBrazil

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