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Neurochemical Research

, Volume 43, Issue 6, pp 1171–1181 | Cite as

Evaluation of Neurotransmitter Alterations in Four Distinct Brain Regions After Rapid Eye Movement Sleep Deprivation (REMSD) Induced Mania-Like Behaviour in Swiss Albino Mice

  • Saiful Alom Siddique
  • Thangavel Tamilselvan
  • Manikkannan Vishnupriya
  • Elumalai BalamuruganEmail author
Original Paper
First Online:

Abstract

A number of neurotransmitter systems have been implicated in contributing to the pathology of mood disorders, including those of dopamine (DA), serotonin (5-HT), norepinephrine (NE) and γ-aminobutyric acid (GABA). Rapid eye movement sleep deprivation (REMSD) alters most of the neurotransmitters, which may have adverse behavioural changes and other health consequences like mania and other psychiatric disorders. The exact role of REMSD altered neurotransmitter levels and the manner in which emerging consequences lead to mania-like behaviour is poorly understood. Thus, we sought to verify the levels of neurotransmitter changes after 48, 72 and 96 h of REMSD induced mania-like behaviour in mice. We performed modified multiple platform (MMP) method of depriving the REM sleep and one group maintained as a control. To measure the hyperactivity through locomotion, exploration and behavioural despair, we performed the Open Field Test (OFT) and the Forced Swim Test (FST). Quantitative determinations of DA, 5-HT, NE and GABA concentrations in four distinct brain regions (cerebral cortex, hippocampus, midbrain, and pons) were determined by the spectrofluorimetric method. These experiments showed higher locomotion and increased swimming, struggling/climbing and decreased mobility among REMSD animals as well as disrupted concentrations of the majority of the studied neurotransmitters during REMSD. Our study indicated that REMSD results in mania-like behaviour in mice and associated disruption to neurotransmitter levels, although the exact mechanisms by which these take place remain to be determined.

Keywords

Rapid eye movement sleep deprivation Mania Bipolar disorder Neurotransmitter Modified multiple platform method 
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Notes

Acknowledgements

Department of Science and Technology (DST), INSPIRE division, New Delhi provided financial support as a fellowship (Grant Reference Number—DST/INSPIRE/2014/IF140562) to carry out this research. The funding source had no involvement in the preparation of the article, study design, collection, analysis and interpretation of data, writing of the report or decision to submit the article for publication.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Saiful Alom Siddique
    • 1
  • Thangavel Tamilselvan
    • 1
  • Manikkannan Vishnupriya
    • 1
  • Elumalai Balamurugan
    • 1
    Email author
  1. 1.Department of Biochemistry and Biotechnology, Faculty of ScienceAnnamalai UniversityAnnamalainagarIndia

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