Neurochemical Research

, Volume 43, Issue 4, pp 821–837 | Cite as

The Leptin, Dopamine and Serotonin Receptors in Hypothalamic POMC-Neurons of Normal and Obese Rodents

  • Irina V. Romanova
  • Kira V. Derkach
  • Anastasiya L. Mikhrina
  • Ivan B. Sukhov
  • Elena V. Mikhailova
  • Alexander O. Shpakov
Original Paper


The pro-opiomelanocortin (POMC)-expressing neurons of the hypothalamic arcuate nucleus (ARC) are involved in the control of food intake and metabolic processes. It is assumed that, in addition to leptin, the activity of these neurons is regulated by serotonin and dopamine, but only subtype 2C serotonin receptors (5-HT2CR) was identified earlier on the POMC-neurons. The aim of this work was a comparative study of the localization and number of leptin receptors (LepR), types 1 and 2 dopamine receptors (D1R, D2R), 5-HT1BR and 5-HT2CR on the POMC-neurons and the expression of the genes encoding them in the ARC of the normal and diet-induced obese (DIO) rodents and the agouti mice (A y /a) with the melanocortin obesity. As shown by immunohistochemistry (IHC), all the studied receptors were located on the POMC-immunopositive neurons, and their IHC-content was in agreement with the expression of their genes. In DIO rats the number of D1R and D2R in the POMC-neurons and their expression in the ARC were reduced. In DIO mice the number of D1R and D2R did not change, while the number of LepR and 5-HT2CR was increased, although to a small extent. In the POMC-neurons of agouti mice the number of LepR, D2R, 5-HT1BR and 5-HT2CR was increased, and the D1R number was reduced. Thus, our data demonstrates for the first time the localization of different types of the serotonin and dopamine receptors on the POMC-neurons and a specific pattern of the changes of their number and expression in the DIO and melanocortin obesity.


Hypothalamus Serotonin Dopamine Leptin Pro-opiomelanocortin Agouti mice Obesity POMC neuron 



This work was supported by the Russian Science Foundation (Project No. 16-15-10388).

Supplementary material

11064_2018_2485_MOESM1_ESM.pdf (194 kb)
Supplementary material 1 (PDF 193 KB)
11064_2018_2485_MOESM2_ESM.pdf (120 kb)
Supplementary material 2 (PDF 120 KB)
11064_2018_2485_MOESM3_ESM.pdf (262 kb)
Supplementary material 3 (PDF 261 KB)


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Authors and Affiliations

  1. 1.I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of SciencesSaint PetersburgRussia

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