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Neurochemical Research

, Volume 42, Issue 12, pp 3559–3572 | Cite as

Differential Molecular Targets for Neuroprotective Effect of Chlorogenic Acid and its Related Compounds Against Glutamate Induced Excitotoxicity and Oxidative Stress in Rat Cortical Neurons

  • Olfa Rebai
  • Manel Belkhir
  • María Victoria Sanchez-Gomez
  • Carlos Matute
  • Sami Fattouch
  • Mohamed Amri
Original Paper

Abstract

The present study has been designed to explore the molecular mechanism and signaling pathway targets of chlorogenic acid (CGA) and its main hydrolysates, caffeic (CA) and quinic acid in the protective effect against glutamate-excitotoxicity. For this purpose 8-DIV cortical neurons in primary culture were exposed to 50 μM l-glutamic acid plus 10 µM glycine, with or without 10–100 μM tested compounds. Chlorogenic acid and caffeic acid via their antioxidant properties inhibited cell death induced by glutamate in dose depended manner. However, quinic acid slightly protects neurons at a higher dose. DCF, JC-1 and Ca2+sensitive fluorescent dye fura-2, were used to measure intracellular ROS accumulation, mitochondrial membrane potential integration and intracellular calcium concentration [Ca2+] i . Results indicate that similarly, CGA acts as a protective agent against glutamate-induced cortical neurons injury by suppressing the accumulation of endogenous ROS and restore the mitochondrial membrane potential, activate the enzymatic antioxidant system by the increase levels of SOD activity and modulate the rise of intracellular calcium levels by increasing the rise of intracellular concentrations of Ca2+caused by glutamate overstimulation. PKC signaling cascade appear to be engaged in this protective mechanism. Interseling, CGA and CA also exhibit antiapoptotic properties against glutamate-induced cleaved activation of pro-caspases; caspase 1,8 and 9 and calpain (PD 150606,Calpeptin and MDL 28170).These data suggest that neuroprotective activity of CGA ester may occurs throught its hydrolysate,the caffeic acid and its interaction with intracellular molecules suggesting that CGA exert its neuroprotection via its caffeoly acid group that might potentially be used as a therapeutic agent in neurodegeneratives disorders associated with glutamate excitotoxicity.

Keywords

Glutamate Neuroprotection Chlorogenic acid Antioxidant Apoptosis Molecular mechanisms 

Abbreviations

CNS

Central nervous system

DCF

Dichlorofluorescein

FDA

Fluorescein diacetate

H2O2

Hydrogen peroxide

LDH

Lactate dehydrogenase

PKA

Protein kinase A

PKC

Protein kinase C

PLC

Phospholipase C

ROS

Reactive oxygen species

MAPK

Mitogen apoptotic protein kinase

DPPH

2, 2-diphenyl-1-picrylhydrazyl

DCF

2’, 7’-dichlorofluorescein

CGA

Chlorogenic acid

CA

Caffeic

QA

Quinic acid

PBS

Phosphate-buffered saline

LDH

Lactate deshydrogenase

CAT

Catalase

SOD

Superoxide dismutase

NMDA

N-methyl-d-aspartate

DMSO

Dimethyl sulfoxide

HBSS

Hank’s Balanced Salt Solution

Notes

Acknowledgements

This work was supported by the Research Unit 00-UR-08-01 University of Sciences, Tunis and by a grant from the Tunisian, Ministry of Higher Education and Scientific Research Tunisia. The authors would like to thank Prof. Matute Carlos from the Instituto Del pays Vasco, Spain for their supportive advices. We acknowledge the financial support from the Ministry of Higher Education and Scientific Research (Tunisia).

References

  1. 1.
    Martindale JL, Holbrook NJ (2002) Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol 192:1–15CrossRefPubMedGoogle Scholar
  2. 2.
    Blandini F, Greenamyre JT, Nappi G (1996) The role of glutamate in the pathophysiology of Parkinson’s disease. Funct Neurol 11:3–15PubMedGoogle Scholar
  3. 3.
    Zeron MM, Chen N, Moshaver A, Lee AT, Wellington CL, Raymond MR (2001) Mutant huntingtin enhances excitotoxic cell death. Mol Cell Neurosci 17:41–53CrossRefPubMedGoogle Scholar
  4. 4.
    Choi DW (1988) Glutamate neurotoxicity and diseases of the nervous system. Neuron 1:623–634CrossRefPubMedGoogle Scholar
  5. 5.
    Dos Santos MD, Almeida MC, Lopes NP, De Souza G (2006) Evaluation of the antiinflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull 29:2236–2240CrossRefPubMedGoogle Scholar
  6. 6.
    Kwon SH, Lee HK, Kim JA, Hong SI, Kim HC, Park YI, Lee CK, Lee YB, Lee SY, Jang CG (2010) Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice. Eur J Pharmacol 649:210–217CrossRefPubMedGoogle Scholar
  7. 7.
    Kim J, Lee S, Shim J, Kim HW, Kim J, Young JJ, Yang H, Park J, Choi SH, Yoon JH, Lee KW, Lee HJ (2012) Caffeinated coffee, decaffeinated coffee, and the phenolic phytochemical chlorogenic acid up-regulate NQO1 expression and prevent H2O2-induced apoptosis in primary cortical neurons. Neurochem Int 60:466–474CrossRefPubMedGoogle Scholar
  8. 8.
    Mattila P, Pihlava JM, Hellstrom J (2005) Contents of phenolic acids, alkyl- and alkenylresorcinols, and avenanthramides in commercial grain products. J Agric Food Chem 53:8290–8295CrossRefPubMedGoogle Scholar
  9. 9.
    Pavlica S, Gebhardt R (2005) Protective effects of ellagic and chlorogenic acids against oxidative stress in PC12 cells. Free Radic Res 39:1377–1390CrossRefPubMedGoogle Scholar
  10. 10.
    Vauzour D, Corona G, Spencer JPE (2010) Caffeic acid, tyrosol and p-coumaric acid are potent inhibitors of 5-S-cysteinyl-dopamine induced neurotoxicity. Arch Biochem Biophys 501(1):106–111CrossRefPubMedGoogle Scholar
  11. 11.
    Vauzour D, Vafeiadou K, Spencer J JPE (2007) Inhibition of the formation of the neurotoxin 5-S-cysteinyl-dopamine by polyphenols. Biochem Biophys Res Commun 362(2):340–346CrossRefPubMedGoogle Scholar
  12. 12.
    Yang JQ, Zhou QX, Liu BZ, He BC (2008) Protection of mouse brain from aluminum-induced damage by caffeic acid. CNS Neurosci Ther 14(1):10–16CrossRefPubMedGoogle Scholar
  13. 13.
    Zhou Y, Fang SH, Ye YL, Chu LS, Zhang WP, Wang ML, Wei EQ (2006) Caffeic acid ameliorates early and delayed brain injuries after focal cerebral ischemia in rats. Acta Pharmacol Sin 27:1103–1110CrossRefPubMedGoogle Scholar
  14. 14.
    Kalonia H et al (2009) Effect of caffeic acid and rofecoxib and their combination against intrastriatal quinolinic acid induced oxidative damage, mitochondrial and histological alterations in rats. Inflammopharmacology 17:211–219CrossRefPubMedGoogle Scholar
  15. 15.
    Hur JY, Soh Y, Kim BH, Suk K, Sohn NW, Kim HC, Kwon HC, Lee KR, Kim SY (2001) Neuroprotective and neurotrophic effects of quinic acids from Aster scaber in PC12 cells. Biol Pharm Bull 24:921–924CrossRefPubMedGoogle Scholar
  16. 16.
    Taram F, Winter AN, Linseman DA (2016) Neuroprotection comparison of chlorogenic acid and its metabolites against mechanistically distinct cell death-inducing agent sin cultured cerebellar granule neurons. Brain Res 1648:69–80CrossRefPubMedGoogle Scholar
  17. 17.
    Mikami Y, Yamazawa T (2015)Chlorogenic acid, a polyphenol in coffee, protects neurons against glutamate neurotoxicity. Life Sci 139:69–74CrossRefPubMedGoogle Scholar
  18. 18.
    Azuma K, Ippoushi K, Nakayama M et al (2000) Absorption of chlorogenic acid and caffeic acid in rats after oral administration. J Agric Food Chem 48:5496–5500CrossRefPubMedGoogle Scholar
  19. 19.
    Zhang YM, Bhavnani B (2005) Glutamate-induced apoptosis in primary cortical neurons is inhibited by equine estrogens via down-regulation of caspase-3 and prevention of mitochondrial cytochrome c release. BMC Neurosci 6:13. https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-6-13
  20. 20.
    Olney JW (1969) Glutamate-induced retinal degeneration in neonatal mice. Electron microscopy of the acutely evolving lesion. J Neuropathol Exp Neurol 28:455–474CrossRefPubMedGoogle Scholar
  21. 21.
    Anggreani E, Lee CY (2017) Neuroprotective effect of chlorogenic acids against Alzheimer’s disease. Int J Food Sci Nutr Diet 6(1):330–337Google Scholar
  22. 22.
    Dupas C, Baglieri AM, Ordonaud C, Tom D, Maillard M (2006) Chlorogenic acid is poorly absorbed, independently of the food matrix: a Caco-2 cells and rat chronic absorption study. Mol Nutr Food Res 50:1053–1060CrossRefPubMedGoogle Scholar
  23. 23.
    Campos-Esparza R, Sanchez-Gomez MV, Matute C (2009) Molecular mechanisms of neuroprotection by two natural antioxidant polyphenols. Cell Calcium 45:358–368CrossRefPubMedGoogle Scholar
  24. 24.
    Ibarretxe G, Sánchez-Gómez MV, Campos-Esparza MR, Alberdi E, Matute C (2006) Differential oxidative stress in oligodendrocytes and neurons after excitotoxic insults and protection by natural polyphénols. Glia 53(2):201–211CrossRefPubMedGoogle Scholar
  25. 25.
    RiceEvanc C (2001) Flavonoid-antioxidant. Curr Med Chem 8:797–807CrossRefGoogle Scholar
  26. 26.
    Gottlieb M et al (2006) Neuroprotection by two polyphenols following excitotoxicity and experimental ischemia. Neurobiol Dis 23:374–386CrossRefPubMedGoogle Scholar
  27. 27.
    Kono Y, Kobayashi K, Tagawa S, Adachi K, Ueda A, Sawa Y, Shibata H (1997) Antioxidant activity of polyphenolics in diets. Rate constants of reactions of chlorogenic acid and caffeic acid with reactive species of oxygen and nitrogen. Biochim Biophys Acta 1335(3):335–342CrossRefPubMedGoogle Scholar
  28. 28.
    Rice-Evans CA, Miller JM, Paganga G (1996) Structure-antioxidant activity relationship of flavonoids and phenolic acids. Free Radic Biol Med 20:933–956CrossRefPubMedGoogle Scholar
  29. 29.
    Sroka Z, Cisowski W (2003) Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol 41:753–758CrossRefPubMedGoogle Scholar
  30. 30.
    Marinova EM, Toneva A, Yanishlieva N (2009) Comparison of the antioxidative properties of caffeic and chlorogenic acids. Food Chem 114:1498–1502CrossRefGoogle Scholar
  31. 31.
    Nardini M, D’Aquino M, Tomassi G, Gentili V, Di Felice M, Scaccini C (1995) Inhibition of human low-density lipoprotein oxidation by caffeic acid and other hydroxycinnamic acid derivatives. Free Radic Biol Med 19:541–552CrossRefPubMedGoogle Scholar
  32. 32.
    Hung TM, Na M, Thuong PT, Su ND, Sok D, Song KS, Seong YH, Bae K (2006) Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall. J Ethnopharmacol 108:188–192CrossRefPubMedGoogle Scholar
  33. 33.
    Genaro-Mattos TC, Maurício ÂQ, Rettori D, Alonso A, Hermes-Lima M (2015) Antioxidant activity of caffeic acid against iron-induced free radical generation—A chemical approach. PLoS ONE 10(11):14624CrossRefGoogle Scholar
  34. 34.
    Andjelkovi M, Van Camp J, De Meulenaer B, Depaemelaere G, Socaciu C, Verloo M, Verhe R (2006) Iron-chelation properties of phenolic acids bearing catechol and galloyl groups. Food Chem 98:23–31CrossRefGoogle Scholar
  35. 35.
    Heiss WD, Kessler J, Mielke R, Szelies B et al (1994) Long-term effects of phosphatidylserine, pyritinol, and cognitive training in Alzheimer’s disease: a neuropsychological EEG, PET investigation. Dementia 5:88–98PubMedGoogle Scholar
  36. 36.
    Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Biol 49:249–279CrossRefGoogle Scholar
  37. 37.
    Matés JM (2000) Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology 153:83–104CrossRefPubMedGoogle Scholar
  38. 38.
    Hopper RK, Carroll S, Aponte AM, Johnson DT, French S, Shen RF, Witzmann FA, Harris RA, Balaban RS (2006) Mitochondrial matrix phosphoproteome: effect of extra mitochondrial calcium. Biochemistry 45:2524–2536CrossRefPubMedPubMedCentralGoogle Scholar
  39. 39.
    Kumar S, Suryanarayanan A, Boyd KN, Comerford CE, Lai MA, Ren Q, Morrow AL (2010) Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase C gamma-dependent mechanism in cultured cerebral cortical neurons. Mol Pharmacol 77:793–803CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Alkon DL, Sun MK, Nelson TJ (2007) PKC signaling deficits: a mechanistic hypothesis for the origins of Alzheimer’s disease. Trends Pharmacol Sci 28:51–60CrossRefPubMedGoogle Scholar
  41. 41.
    Lin TY, Chung CY, Lu CW, Huang SK, Shieh JS, Wang SJ (2013) Local anesthetics inhibit glutamate release from rat cerebral cortex synaptosomes. Synapse 67:568–579CrossRefPubMedGoogle Scholar
  42. 42.
    Orrenius S (2008) Reactive oxygen species in mitochondria-mediated cell death. Drug Metab Rev 39:443–455CrossRefGoogle Scholar
  43. 43.
    White RJ, Reynolds IJ (1996) Mitochondrial depolarization in glutamate-stimulated neurons: an early signal specific to excitotoxin exposure. J Neurosci 16(18):5688–5697PubMedGoogle Scholar
  44. 44.
    Poręba M, Stróżyk A, Salvesen GS, Drąg M (2013) Caspase substrates and inhibitors. Cold Spring Harb Perspect Biol 5(8). doi: 10.1101/cshperspect.a008680
  45. 45.
    Liu H, Radhakrishna B (2005) Endoplasmic reticulum stress–associated caspase 12 mediates cisplatin-induced LLC-PK1 cell apoptosis. JASN Express 16:1985–1992CrossRefGoogle Scholar
  46. 46.
    Yuan B, Yankner A (2000) Apoptosis in the nervous system. Nature 407:802–809CrossRefPubMedGoogle Scholar
  47. 47.
    Earnshaw WC, Martins LM, Kaufmann SH (1999) Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem 68:383–424CrossRefPubMedGoogle Scholar
  48. 48.
    Leon R, Wu H, Jin Y, Wei J, Buddhala C, Prentice H, Wu JY (2009) Protective function of taurine in glutamate-induced apoptosis in cultured neurons. J Neurosci Res 87(5):1185–1194CrossRefPubMedGoogle Scholar
  49. 49.
    Wang KW (2000) Calpain and caspases: can you tell the difference. Trends Neurosci 23:20–26CrossRefPubMedGoogle Scholar
  50. 50.
    Wingrave JM, Schaecher KE, Sribnick EA, Wilford GG, Ray SK, Hazen-Martin DJ, Hogan EL, Banik L (2003) Early induction of secondary injury factors causing activation of calpain and mitochondria-mediated neuronal apoptosis following spinal cord injury in rats. J Neurosci Res 73(1):95–104CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Olfa Rebai
    • 1
  • Manel Belkhir
    • 1
  • María Victoria Sanchez-Gomez
    • 3
  • Carlos Matute
    • 3
  • Sami Fattouch
    • 2
  • Mohamed Amri
    • 1
  1. 1.Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Department of Biological Sciences, Faculty of Science of TunisUniversity of Tunis El ManarTunisTunisia
  2. 2.Laboratoire de Biochimie Alimentaire, INSATUniversity of CarthageTunisTunisia
  3. 3.Departamento de Neurociencias, Facultad de Medicina y OdontologiaUniversidad Del Paıs VascoLeioaSpain

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