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Neurochemical Research

, Volume 42, Issue 5, pp 1459–1469 | Cite as

β-Caryophyllene Pretreatment Alleviates Focal Cerebral Ischemia-Reperfusion Injury by Activating PI3K/Akt Signaling Pathway

  • Qian Zhang
  • Ruidi An
  • Xiaocui Tian
  • Mei Yang
  • Minghang Li
  • Jie Lou
  • Lu XuEmail author
  • Zhi DongEmail author
Original Paper

Abstract

β-Caryophyllene (BCP) has been reported to be protective against focal cerebral ischemia–reperfusion (I/R) injury by its anti-oxidative and anti-inflammatory features. Recent study demonstrates that the BCP exhibits potential neuroprotection against I/R injury induced apoptosis, however, the mechanism remains unknown. Therefore, we investigate the underlying anti-apoptotic mechanism of BCP pretreatment in I/R injury. Sprague–Dawley rats (pretreated with BCP suspensions or solvent orally for 7 days) were subjected to transient Middle Cerebral Artery Occlusion (MCAO) for 90 min, followed by 24 h reperfusion. Results showed that BCP pretreatment improved the neurologic deficit score, lowered the infarct volume and decreased number of apoptotic cells in the hippocampus. Moreover, in western blot and RT-qPCR detections, BCP pretreatment down-regulated the expressions of Bax and p53, up-regulated the expression of Bcl-2, and enhanced the phosphorylation of Akt on Ser473. Blockage of PI3K activity by wortmannin not only abolished the BCP-induced decreases in infarct volume and neurologic deficit score, but also dramatically abrogated the enhancement of AKt phosphorylation. Our results suggested that BCP pre-treatment protects against I/R injury partly by suppressing apoptosis via PI3K/AKt signaling pathway activation.

Keywords

β-Caryophyllene Ischemic-reperfusion injury Neuroprotection Apoptosis PI3K/Akt signaling pathway 

Notes

Funding

Funding was provided by Chongqing Research Program of Basic Research and Frontier Technology (No. cstc2016jcyjA0373).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, School of PharmacyChongqing Medical UniversityChongqingChina

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